Engineering monovalent quantum dot-antibody bioconjugates with a hybrid gel system

HongYan Liu, Xiaohu Gao

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Monovalent nanoparticles are of strong current interest in biological imaging and detection due to their potential for stoichiometric binding with target molecules. We report the preparation of monovalent quantum dot-antibody bioconjugates using a high-resolution hybrid gel system specially designed for fractionation of nanoparticle bioconjugates. A key feature of this technology is that it is broadly applicable to many types of nanoparticle-antibody complexes without the need of genetically engineered proteins. This is particularly important because antibodies are still the dominant molecular targeting probes, despite new discoveries made with other targeting probes such as aptamers and peptides. Furthermore, we show experimental evidence of improved quantification capability using the monovalent probes, whose advantages over their multivalent counterparts had largely been a theoretic prediction previously. This new class of nanoprobe should find broad application in quantitative biological detection and imaging in vitro and in vivo.

Original languageEnglish (US)
Pages (from-to)510-517
Number of pages8
JournalBioconjugate Chemistry
Volume22
Issue number3
DOIs
StatePublished - Mar 16 2011
Externally publishedYes

Fingerprint

Quantum Dots
Antibodies
Nanoparticles
Semiconductor quantum dots
Gels
Peptide Aptamers
Nanoprobes
Imaging techniques
Molecular Probes
Fractionation
Peptides
Technology
Proteins
Molecules

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Organic Chemistry
  • Pharmaceutical Science
  • Biomedical Engineering
  • Pharmacology

Cite this

Engineering monovalent quantum dot-antibody bioconjugates with a hybrid gel system. / Liu, HongYan; Gao, Xiaohu.

In: Bioconjugate Chemistry, Vol. 22, No. 3, 16.03.2011, p. 510-517.

Research output: Contribution to journalArticle

Liu, HongYan ; Gao, Xiaohu. / Engineering monovalent quantum dot-antibody bioconjugates with a hybrid gel system. In: Bioconjugate Chemistry. 2011 ; Vol. 22, No. 3. pp. 510-517.
@article{3f9eabd311114429950a25349b89de71,
title = "Engineering monovalent quantum dot-antibody bioconjugates with a hybrid gel system",
abstract = "Monovalent nanoparticles are of strong current interest in biological imaging and detection due to their potential for stoichiometric binding with target molecules. We report the preparation of monovalent quantum dot-antibody bioconjugates using a high-resolution hybrid gel system specially designed for fractionation of nanoparticle bioconjugates. A key feature of this technology is that it is broadly applicable to many types of nanoparticle-antibody complexes without the need of genetically engineered proteins. This is particularly important because antibodies are still the dominant molecular targeting probes, despite new discoveries made with other targeting probes such as aptamers and peptides. Furthermore, we show experimental evidence of improved quantification capability using the monovalent probes, whose advantages over their multivalent counterparts had largely been a theoretic prediction previously. This new class of nanoprobe should find broad application in quantitative biological detection and imaging in vitro and in vivo.",
author = "HongYan Liu and Xiaohu Gao",
year = "2011",
month = "3",
day = "16",
doi = "10.1021/bc200004z",
language = "English (US)",
volume = "22",
pages = "510--517",
journal = "Bioconjugate Chemistry",
issn = "1043-1802",
publisher = "American Chemical Society",
number = "3",

}

TY - JOUR

T1 - Engineering monovalent quantum dot-antibody bioconjugates with a hybrid gel system

AU - Liu, HongYan

AU - Gao, Xiaohu

PY - 2011/3/16

Y1 - 2011/3/16

N2 - Monovalent nanoparticles are of strong current interest in biological imaging and detection due to their potential for stoichiometric binding with target molecules. We report the preparation of monovalent quantum dot-antibody bioconjugates using a high-resolution hybrid gel system specially designed for fractionation of nanoparticle bioconjugates. A key feature of this technology is that it is broadly applicable to many types of nanoparticle-antibody complexes without the need of genetically engineered proteins. This is particularly important because antibodies are still the dominant molecular targeting probes, despite new discoveries made with other targeting probes such as aptamers and peptides. Furthermore, we show experimental evidence of improved quantification capability using the monovalent probes, whose advantages over their multivalent counterparts had largely been a theoretic prediction previously. This new class of nanoprobe should find broad application in quantitative biological detection and imaging in vitro and in vivo.

AB - Monovalent nanoparticles are of strong current interest in biological imaging and detection due to their potential for stoichiometric binding with target molecules. We report the preparation of monovalent quantum dot-antibody bioconjugates using a high-resolution hybrid gel system specially designed for fractionation of nanoparticle bioconjugates. A key feature of this technology is that it is broadly applicable to many types of nanoparticle-antibody complexes without the need of genetically engineered proteins. This is particularly important because antibodies are still the dominant molecular targeting probes, despite new discoveries made with other targeting probes such as aptamers and peptides. Furthermore, we show experimental evidence of improved quantification capability using the monovalent probes, whose advantages over their multivalent counterparts had largely been a theoretic prediction previously. This new class of nanoprobe should find broad application in quantitative biological detection and imaging in vitro and in vivo.

UR - http://www.scopus.com/inward/record.url?scp=79952778730&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952778730&partnerID=8YFLogxK

U2 - 10.1021/bc200004z

DO - 10.1021/bc200004z

M3 - Article

C2 - 21348524

AN - SCOPUS:79952778730

VL - 22

SP - 510

EP - 517

JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

SN - 1043-1802

IS - 3

ER -