Enhanced cytotoxic potential of alveolar macrophages from cigarette smokers

W. B. Davis, E. R. Pacht, M. Spatafora, W. J. Martin

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Cigarette smoking increases the numbers and oxidative metabolism of alveolar macrophages. Increased production of Superoxide (O2-) and H2O2 by alveolar macrophages may contribute to the pathogenesis of cigarette-induced lung diseases. The cytotoxlcity mediated by alveolar macrophages from smokers (n = 11) and nonsmokers (n = 13) was compared in an in vitro assay in which the target cells were chromium 51-labeled lung expiants. The spontaneous cellular cytotoxiclty mediated by smoker macrophages was significantly greater than that of nonsmoker macrophages (cytotoxic index 20.3% ± 1.9% compared with 5.5% ± 0.9%, P < 0.001). Phorbol myristate acetate significantly increased the cytotoxic index of nonsmoker macrophages but did not cause further increases in smoker macrophage killing. The antioxidants Superoxide dismutase and catalase produced partial inhibition of smoker macrophage cytotoxicity, suggesting that target cell killing was mediated in part by oxidant mechanisms. Supplementation of smokers' diets with high-dose oral vitamin E failed to decrease smoker alveolar macrophage cytotoxicity. These findings demonstrate that smoker alveolar macrophages possess enhanced cytotoxic potential for normal lung parenchymal cells.

Original languageEnglish (US)
Pages (from-to)293-298
Number of pages6
JournalJournal of Laboratory and Clinical Medicine
Volume111
Issue number3
StatePublished - Jan 1 1988

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Macrophages
Alveolar Macrophages
Tobacco Products
Cytotoxicity
Lung
Pulmonary diseases
Tetradecanoylphorbol Acetate
Chromium
Nutrition
Vitamin E
Oxidants
Metabolism
Superoxides
Catalase
Lung Diseases
Superoxide Dismutase
Assays
Antioxidants
Smoking
Cells

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

Enhanced cytotoxic potential of alveolar macrophages from cigarette smokers. / Davis, W. B.; Pacht, E. R.; Spatafora, M.; Martin, W. J.

In: Journal of Laboratory and Clinical Medicine, Vol. 111, No. 3, 01.01.1988, p. 293-298.

Research output: Contribution to journalArticle

Davis, W. B. ; Pacht, E. R. ; Spatafora, M. ; Martin, W. J. / Enhanced cytotoxic potential of alveolar macrophages from cigarette smokers. In: Journal of Laboratory and Clinical Medicine. 1988 ; Vol. 111, No. 3. pp. 293-298.
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