TY - JOUR
T1 - Enhanced establishment of a virus carrier state in adult CD4+ T-cell-dencient mice
AU - Battegay, Manuel
AU - Moskophidis, Demetrius
AU - Rahemtulla, Amin
AU - Hengartner, Hans
AU - Mak, Tak W.
AU - Zinkernagel, Rolf M.
PY - 1994/7
Y1 - 1994/7
N2 - CD4+ T cells play an important role in regulating the immune response; their contribution to virus clearance is variable. Mice that lack CD4+ T cells (CD4-/- mice) and are therefore unable to produce neutralizing antibodies cleared viscero-lymphotropic lymphocytic choriomeningitis virus (LCMV) strain WE when infected intravenously with a low dose (2 × 102 PFU) because of an effective CD8+ cytotoxic T-cell (CTL) response. In contrast, infection with a high dose (2 × 106 PFU) of LCMV strain WE led to expansion of antiviral CTL, which disappeared in CD4-/- mice; in contrast, CD4+ T-cell-competent mice developed antiviral memory CTL. This exhaustion of specific CTL caused viral persistence in CD4-/- mice, whereas CD4+ T-cell-competent mice eliminated the virus. After infection of CD4-/- mice with the faster-replicating LCMV strain DOCILE, abrogation of CTL response and establishment of viral persistence developed after infection with a low dose (5 × 102 PFU), i.e., an about 100-fold lower dose than in CD4+-competent control mice. These results show that absence of T help enhances establishment of an LCMV carrier state in selected situations.
AB - CD4+ T cells play an important role in regulating the immune response; their contribution to virus clearance is variable. Mice that lack CD4+ T cells (CD4-/- mice) and are therefore unable to produce neutralizing antibodies cleared viscero-lymphotropic lymphocytic choriomeningitis virus (LCMV) strain WE when infected intravenously with a low dose (2 × 102 PFU) because of an effective CD8+ cytotoxic T-cell (CTL) response. In contrast, infection with a high dose (2 × 106 PFU) of LCMV strain WE led to expansion of antiviral CTL, which disappeared in CD4-/- mice; in contrast, CD4+ T-cell-competent mice developed antiviral memory CTL. This exhaustion of specific CTL caused viral persistence in CD4-/- mice, whereas CD4+ T-cell-competent mice eliminated the virus. After infection of CD4-/- mice with the faster-replicating LCMV strain DOCILE, abrogation of CTL response and establishment of viral persistence developed after infection with a low dose (5 × 102 PFU), i.e., an about 100-fold lower dose than in CD4+-competent control mice. These results show that absence of T help enhances establishment of an LCMV carrier state in selected situations.
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M3 - Article
C2 - 7911534
AN - SCOPUS:0028298652
SN - 0022-538X
VL - 68
SP - 4700
EP - 4704
JO - Journal of Virology
JF - Journal of Virology
IS - 7
ER -