Enhanced matrix metalloproteinase expression by Tisseel in mesothelial cells, normal peritoneal fibroblasts, and adhesion fibroblasts

Ghassan M. Saed, Michael Kruger, Michael Peter Diamond

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Matrix metalloproteinases (MMPs) and tissue inhibitors of matrix metalloproteinases (TIMPs) are important in regulating tissue remodeling of extracellular matrix. Dysregulation of MMPs is considered to be important to postoperative adhesion development. To examine whether Tisseel, a fibrin sealant, exerts an effect on expression of MMPs from human peritoneal mesothelial cells, normal peritoneal fibroblasts, and adhesion fibroblasts, a tissue culture study was conducted for 6, 12, 24, and 48 h under normoxic conditions. A multiplex reverse transcriptase-polymerase chain reaction technique using β-actin as a housekeeping gene was used to determine relative change in MMP-1, MMP-2, and TIMP-1 mRNA levels under six conditions (five fibrin sealant preparations and a control). Our results demonstrate that MMP-1, MMP-2, and TIMP-1 are all expressed by human peritoneal mesothelial cells and fibroblasts. All fibrin sealant compositions increased MMP-1 mRNA levels by all cell types, although the time course and pattern varied. The fibrin sealant compositions had variable effects on MMP-2 mRNA expression. Interestingly, in mesothelial cells, TIMP-1 mRNA levels were reduced by fibrin-sealant-containing aprotinin at both concentrations, while being further reduced by fibrin sealant without aprotinin, particularly at 6 and 12 h. In adhesion fibroblasts, there was generally no effect, while in normal fibroblasts, expression of TIMP-1 increased after 24 h. These results suggest that fibrin sealant, in addition to assisting in the achievement of hemostasis, exerts biological effects on human peritoneal mesothelial cells and fibroblasts and modulates MMP expression. Thus, fibrin sealant may influence tissue remodeling and may thereby be able to alter postoperative adhesion development.

Original languageEnglish (US)
Pages (from-to)472-479
Number of pages8
JournalEuropean Journal of Plastic Surgery
Volume28
Issue number7
DOIs
StatePublished - Feb 1 2006

Fingerprint

Fibrin Tissue Adhesive
Matrix Metalloproteinases
Matrix Metalloproteinase 1
Fibroblasts
Tissue Inhibitor of Metalloproteinase-1
Matrix Metalloproteinase Inhibitors
Matrix Metalloproteinase 2
Messenger RNA
Aprotinin
Tissue Inhibitor of Metalloproteinases
Multiplex Polymerase Chain Reaction
Essential Genes
Hemostasis
Reverse Transcriptase Polymerase Chain Reaction
Extracellular Matrix
Actins
Epithelium

Keywords

  • Adhesions
  • Fibrin sealant
  • MMP-1
  • MMP-2
  • TIMP-1
  • Tisseel

ASJC Scopus subject areas

  • Surgery

Cite this

Enhanced matrix metalloproteinase expression by Tisseel in mesothelial cells, normal peritoneal fibroblasts, and adhesion fibroblasts. / Saed, Ghassan M.; Kruger, Michael; Diamond, Michael Peter.

In: European Journal of Plastic Surgery, Vol. 28, No. 7, 01.02.2006, p. 472-479.

Research output: Contribution to journalArticle

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