Enhancing the activity of insulin at the receptor interface

Crystal structure and photo-cross-linking of A8 analogues

Zhuli Wan, Bin Xu, Kun Huang, Ying Chi Chu, Biaoru Li, Satoe H. Nakagawa, Yan Qu, Shi Quan Hu, Panayotis G. Katsoyannis, Michael A. Weiss

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The receptor-binding surface of insulin is broadly conserved, reflecting its evolutionary optimization. Neighboring positions nevertheless offer an opportunity to enhance activity, through either transmitted structural changes or introduction of novel contacts. Nonconserved residue A8 is of particular interest as ThrA8 → His substitution (a species variant in birds and fish) augments the potency of human insulin. Diverse A8 substitutions are well tolerated, suggesting that the hormone-receptor interface is not tightly packed at this site. To resolve whether enhanced activity is directly or indirectly mediated by the variant A8 side chain, we have determined the crystal structure of HisA8-insulin and investigated the photo-cross-linking properties of an A8 analogue containing p-azidophenylalanine. The structure, characterized as a T3R3f zinc hexamer at 1.8 Å resolution, is essentially identical to that of native insulin. The photoactivatable analogue exhibits efficient cross-linking to the insulin receptor. The site of cross-linking lies within a 14 kDa C-terminal domain of the α-subunit. This contact, to our knowledge the first to be demonstrated from the A chain, is inconsistent with a recent model of the hormone-receptor complex derived from electron microscopy. Optimizing the binding interaction of a nonconserved side chain on the surface of insulin may thus enhance its activity.

Original languageEnglish (US)
Pages (from-to)16119-16133
Number of pages15
JournalBiochemistry
Volume43
Issue number51
DOIs
StatePublished - Dec 28 2004
Externally publishedYes

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Insulin Receptor
Crystal structure
Insulin
Substitution reactions
Hormones
Birds
Fish
Electron microscopy
Zinc
Electron Microscopy
Fishes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Enhancing the activity of insulin at the receptor interface : Crystal structure and photo-cross-linking of A8 analogues. / Wan, Zhuli; Xu, Bin; Huang, Kun; Chu, Ying Chi; Li, Biaoru; Nakagawa, Satoe H.; Qu, Yan; Hu, Shi Quan; Katsoyannis, Panayotis G.; Weiss, Michael A.

In: Biochemistry, Vol. 43, No. 51, 28.12.2004, p. 16119-16133.

Research output: Contribution to journalArticle

Wan, Z, Xu, B, Huang, K, Chu, YC, Li, B, Nakagawa, SH, Qu, Y, Hu, SQ, Katsoyannis, PG & Weiss, MA 2004, 'Enhancing the activity of insulin at the receptor interface: Crystal structure and photo-cross-linking of A8 analogues', Biochemistry, vol. 43, no. 51, pp. 16119-16133. https://doi.org/10.1021/bi048223f
Wan, Zhuli ; Xu, Bin ; Huang, Kun ; Chu, Ying Chi ; Li, Biaoru ; Nakagawa, Satoe H. ; Qu, Yan ; Hu, Shi Quan ; Katsoyannis, Panayotis G. ; Weiss, Michael A. / Enhancing the activity of insulin at the receptor interface : Crystal structure and photo-cross-linking of A8 analogues. In: Biochemistry. 2004 ; Vol. 43, No. 51. pp. 16119-16133.
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