Enumeration of single IFN-γ-producing cells in mice during viral and bacterial infection

A. Gessner, Dimitrios Moskofidis, F. Lehmann-Grube

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

A solid phas immunoenzymatic technique was employed for detecting single IFN-γ-producing cells (IFN-γPC) in the mouse. After infection with lymphocytic choriomeningitis virus or Listeria monocytogenes, the numbers of IFN-γPC in spleens began to rise on day 4, attained maxima on days 7 and 8, and declined thereafter. Negative selection in vitro by use of mAb and C allowed phenotypic identification of the producer cells; most, if not all, carried Thy-1, and approximately one half expressed CD4, the other half, CD8. Depletion of cells in vivo by treatment of mice with mAb led to somewhat different results; again, anti-Thy-1 antibody eliminated essentially all IFN-γPC, but considerably more than 50% were either CD4+ or CD8+, suggesting regulatory interactions between these T lymphocyte subsets with regard to generation of the lymphokine.

Original languageEnglish (US)
Pages (from-to)1293-1298
Number of pages6
JournalJournal of Immunology
Volume142
Issue number4
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

Virus Diseases
Bacterial Infections
Lymphocytic choriomeningitis virus
Lymphokines
Listeria monocytogenes
T-Lymphocyte Subsets
Spleen
Infection
anti-Thy antibody

ASJC Scopus subject areas

  • Immunology

Cite this

Enumeration of single IFN-γ-producing cells in mice during viral and bacterial infection. / Gessner, A.; Moskofidis, Dimitrios; Lehmann-Grube, F.

In: Journal of Immunology, Vol. 142, No. 4, 01.01.1989, p. 1293-1298.

Research output: Contribution to journalArticle

@article{440c078138e545eeb2b6c9711bd9df1a,
title = "Enumeration of single IFN-γ-producing cells in mice during viral and bacterial infection",
abstract = "A solid phas immunoenzymatic technique was employed for detecting single IFN-γ-producing cells (IFN-γPC) in the mouse. After infection with lymphocytic choriomeningitis virus or Listeria monocytogenes, the numbers of IFN-γPC in spleens began to rise on day 4, attained maxima on days 7 and 8, and declined thereafter. Negative selection in vitro by use of mAb and C allowed phenotypic identification of the producer cells; most, if not all, carried Thy-1, and approximately one half expressed CD4, the other half, CD8. Depletion of cells in vivo by treatment of mice with mAb led to somewhat different results; again, anti-Thy-1 antibody eliminated essentially all IFN-γPC, but considerably more than 50{\%} were either CD4+ or CD8+, suggesting regulatory interactions between these T lymphocyte subsets with regard to generation of the lymphokine.",
author = "A. Gessner and Dimitrios Moskofidis and F. Lehmann-Grube",
year = "1989",
month = "1",
day = "1",
language = "English (US)",
volume = "142",
pages = "1293--1298",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "4",

}

TY - JOUR

T1 - Enumeration of single IFN-γ-producing cells in mice during viral and bacterial infection

AU - Gessner, A.

AU - Moskofidis, Dimitrios

AU - Lehmann-Grube, F.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - A solid phas immunoenzymatic technique was employed for detecting single IFN-γ-producing cells (IFN-γPC) in the mouse. After infection with lymphocytic choriomeningitis virus or Listeria monocytogenes, the numbers of IFN-γPC in spleens began to rise on day 4, attained maxima on days 7 and 8, and declined thereafter. Negative selection in vitro by use of mAb and C allowed phenotypic identification of the producer cells; most, if not all, carried Thy-1, and approximately one half expressed CD4, the other half, CD8. Depletion of cells in vivo by treatment of mice with mAb led to somewhat different results; again, anti-Thy-1 antibody eliminated essentially all IFN-γPC, but considerably more than 50% were either CD4+ or CD8+, suggesting regulatory interactions between these T lymphocyte subsets with regard to generation of the lymphokine.

AB - A solid phas immunoenzymatic technique was employed for detecting single IFN-γ-producing cells (IFN-γPC) in the mouse. After infection with lymphocytic choriomeningitis virus or Listeria monocytogenes, the numbers of IFN-γPC in spleens began to rise on day 4, attained maxima on days 7 and 8, and declined thereafter. Negative selection in vitro by use of mAb and C allowed phenotypic identification of the producer cells; most, if not all, carried Thy-1, and approximately one half expressed CD4, the other half, CD8. Depletion of cells in vivo by treatment of mice with mAb led to somewhat different results; again, anti-Thy-1 antibody eliminated essentially all IFN-γPC, but considerably more than 50% were either CD4+ or CD8+, suggesting regulatory interactions between these T lymphocyte subsets with regard to generation of the lymphokine.

UR - http://www.scopus.com/inward/record.url?scp=0024561556&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024561556&partnerID=8YFLogxK

M3 - Article

C2 - 2492578

AN - SCOPUS:0024561556

VL - 142

SP - 1293

EP - 1298

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 4

ER -