A solid phas immunoenzymatic technique was employed for detecting single IFN-γ-producing cells (IFN-γPC) in the mouse. After infection with lymphocytic choriomeningitis virus or Listeria monocytogenes, the numbers of IFN-γPC in spleens began to rise on day 4, attained maxima on days 7 and 8, and declined thereafter. Negative selection in vitro by use of mAb and C allowed phenotypic identification of the producer cells; most, if not all, carried Thy-1, and approximately one half expressed CD4, the other half, CD8. Depletion of cells in vivo by treatment of mice with mAb led to somewhat different results; again, anti-Thy-1 antibody eliminated essentially all IFN-γPC, but considerably more than 50% were either CD4+ or CD8+, suggesting regulatory interactions between these T lymphocyte subsets with regard to generation of the lymphokine.
|Original language||English (US)|
|Number of pages||6|
|Journal||Journal of Immunology|
|State||Published - Jan 1 1989|
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