Enzyme modification of platinum microelectrodes for detection of cholesterol in vesicle lipid bilayer membranes

Anando Devadoss, M. Simona Palencsár, Dechen Jiang, Michael L. Honkonen, James Burgess

Research output: Contribution to journalArticle

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Abstract

Platinum microelectrodes are modified with a lipid bilayer membrane incorporating cholesterol oxidase. Details for electrode surface modification are presented along with characterization studies of electrode response to cholesterol solution and to cholesterol contained in the lipid bilayer membrane of vesicles. Ferrocyanide voltammetric experiments are used to track deposition of a submonolayer of a thiol-functionalized lipid on the platinum electrode surface, vesicle fusion for bilayer formation on the thiolipid-modified surface, and incorporation of cholesterol oxidase in the electrode-supported thiolipid/lipid bilayer membrane. The data are consistent with formation of a lipid bilayer structure on the electrode surface that contains defects. Experiments for detection of cholesterol solubilized in cyclodextrin solution show steady-state current responses that correlate with cholesterol concentration. Direct contact between the electrode and a vesicle lipid bilayer membrane shows a response that correlates with vesicle membrane cholesterol content.

Original languageEnglish (US)
Pages (from-to)7393-7398
Number of pages6
JournalAnalytical Chemistry
Volume77
Issue number22
DOIs
StatePublished - Nov 15 2005

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ASJC Scopus subject areas

  • Analytical Chemistry

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