Epidermal growth factor-dependent enhancement of axonal regeneration in the pond snail Lymnaea stagnalis: Role of phagocyte survival

Petra M. Hermann, Jennifer J. Nicol, Gregg Thomas Nagle, Andrew G.M. Bulloch, Willem C. Wildering

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Peripheral nerve injury triggers complex responses from neuronal as well as from multiple nonneuronal cell types. These responses are coordinated by a wide spectrum of secreted and nonsecreted factors, including growth factors, cytokines, and cell adhesion molecules. These molecules originate from different sources and act both locally at the site of injury as well as centrally at the location of the neuronal cell bodies. One of the signal systems frequently implicated in this process is the epidermal growth factor (EGF) family and its receptors. Expression of members of this family as well as that of EGF-receptors is upregulated in different cell types after peripheral nerve injury. However, the functional significance of this response is unclear. Using a simple invertebrate model system (Lymnaea stagnalis), the present study implicates the EGF/EGF-receptor system in the survival of ionized calcium-binding adaptor molecule 1 (Iba1)-positive phagocytes that reside in the nervous system. We show that inhibiting the EGF-signaling pathway enhances cell death in this type of cell, an effect paralleled by a substantial reduction in axonal regeneration. Therefore, complementing our previous observation that Lymnaea EGF provides trophic support to axotomized neurons, the present results emphasize the significance of nonneuronal actions of EGF receptor ligands in axonal regeneration. Thus, we add a novel perspective to the ongoing discussion on the functional significance of the EGF signaling system in the injury responses of the nervous system.

Original languageEnglish (US)
Pages (from-to)383-400
Number of pages18
JournalJournal of Comparative Neurology
Volume492
Issue number4
DOIs
StatePublished - Nov 28 2005
Externally publishedYes

Fingerprint

Lymnaea
Snails
Phagocytes
Epidermal Growth Factor
Regeneration
Peripheral Nerve Injuries
Epidermal Growth Factor Receptor
Nervous System Trauma
Cell Adhesion Molecules
Invertebrates
Nervous System
Intercellular Signaling Peptides and Proteins
Cell Death
Cytokines
Ligands
Calcium
Neurons
Wounds and Injuries

Keywords

  • Epidermal growth factor
  • Iba1
  • Invertebrate
  • Nerve regeneration
  • Neurotrophic factor
  • Phagocytosis
  • Survival

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Epidermal growth factor-dependent enhancement of axonal regeneration in the pond snail Lymnaea stagnalis : Role of phagocyte survival. / Hermann, Petra M.; Nicol, Jennifer J.; Nagle, Gregg Thomas; Bulloch, Andrew G.M.; Wildering, Willem C.

In: Journal of Comparative Neurology, Vol. 492, No. 4, 28.11.2005, p. 383-400.

Research output: Contribution to journalArticle

Hermann, Petra M. ; Nicol, Jennifer J. ; Nagle, Gregg Thomas ; Bulloch, Andrew G.M. ; Wildering, Willem C. / Epidermal growth factor-dependent enhancement of axonal regeneration in the pond snail Lymnaea stagnalis : Role of phagocyte survival. In: Journal of Comparative Neurology. 2005 ; Vol. 492, No. 4. pp. 383-400.
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