Epididymal fat is necessary for spermatogenesis, but not testosterone production or copulatory behavior

Ye Chu, Gloria G. Huddleston, Andrew N. Clancy, Ruth Babette Harris, Timothy J. Bartness

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Surgical removal of the epididymal white adipose tissue (EWAT) depot (lipectomy; EWATx) in laboratory rats or mice decreases spermatogenesis, but this phenomenal finding has not been investigated in depth. Specifically, detailed histology, neuroendocrine profiles, copulatory behavior, lipectomy of other WAT depots, rescue by autologous EWAT transplants, or tests whether this EWATx effect is due to disruption of testes innervation occurring during EWATx have not been performed. Therefore, in the first study, we performed EWATx in male Syrian hamsters and attempted to rescue spermatogenesis by transplanting the removed EWAT to the animal's subcutaneous dorsum, removed comparable or larger amounts of non-gonadal WAT [inguinal WAT (IWAT)] and conducted mating behavior tests. In a second study we conducted detailed testicular histology and assayed serum LH, FSH, and testosterone (T). In a third study, we surgically denervated the testes without removing EWAT and compared testicular histology with that of EWATx or sham surgery. We found that EWATx, but not IWATx, virtually eliminated spermatogenesis producing a marked decrease in size of the seminiferous tubule cellular lining including the Sertoli cells and spermatogonia that could not be rescued by autologous EWAT transplant to the subcutaneous dorsum. EWATx did not change serum LH or T concentrations but approximately doubled serum FSH concentrations. EWATx did not alter copulatory behavior but resulted in aspermatic ejaculate. Selective surgical testicular denervation did not affect spermatogenesis. Collectively, these results suggest the presence of a local, but currently unidentified, growth and/or nutritive factor from EWAT that promotes spermatogenesis.

Original languageEnglish (US)
Pages (from-to)5669-5679
Number of pages11
JournalEndocrinology
Volume151
Issue number12
DOIs
StatePublished - Dec 1 2010

Fingerprint

White Adipose Tissue
Spermatogenesis
Testosterone
Fats
Lipectomy
Histology
Testis
Serum
Transplants
Spermatogonia
Seminiferous Tubules
Groin
Sertoli Cells
Mesocricetus
Denervation
Growth

ASJC Scopus subject areas

  • Endocrinology

Cite this

Epididymal fat is necessary for spermatogenesis, but not testosterone production or copulatory behavior. / Chu, Ye; Huddleston, Gloria G.; Clancy, Andrew N.; Harris, Ruth Babette; Bartness, Timothy J.

In: Endocrinology, Vol. 151, No. 12, 01.12.2010, p. 5669-5679.

Research output: Contribution to journalArticle

Chu, Ye ; Huddleston, Gloria G. ; Clancy, Andrew N. ; Harris, Ruth Babette ; Bartness, Timothy J. / Epididymal fat is necessary for spermatogenesis, but not testosterone production or copulatory behavior. In: Endocrinology. 2010 ; Vol. 151, No. 12. pp. 5669-5679.
@article{99c87fe3402449c3b9854e8ba0d7ea74,
title = "Epididymal fat is necessary for spermatogenesis, but not testosterone production or copulatory behavior",
abstract = "Surgical removal of the epididymal white adipose tissue (EWAT) depot (lipectomy; EWATx) in laboratory rats or mice decreases spermatogenesis, but this phenomenal finding has not been investigated in depth. Specifically, detailed histology, neuroendocrine profiles, copulatory behavior, lipectomy of other WAT depots, rescue by autologous EWAT transplants, or tests whether this EWATx effect is due to disruption of testes innervation occurring during EWATx have not been performed. Therefore, in the first study, we performed EWATx in male Syrian hamsters and attempted to rescue spermatogenesis by transplanting the removed EWAT to the animal's subcutaneous dorsum, removed comparable or larger amounts of non-gonadal WAT [inguinal WAT (IWAT)] and conducted mating behavior tests. In a second study we conducted detailed testicular histology and assayed serum LH, FSH, and testosterone (T). In a third study, we surgically denervated the testes without removing EWAT and compared testicular histology with that of EWATx or sham surgery. We found that EWATx, but not IWATx, virtually eliminated spermatogenesis producing a marked decrease in size of the seminiferous tubule cellular lining including the Sertoli cells and spermatogonia that could not be rescued by autologous EWAT transplant to the subcutaneous dorsum. EWATx did not change serum LH or T concentrations but approximately doubled serum FSH concentrations. EWATx did not alter copulatory behavior but resulted in aspermatic ejaculate. Selective surgical testicular denervation did not affect spermatogenesis. Collectively, these results suggest the presence of a local, but currently unidentified, growth and/or nutritive factor from EWAT that promotes spermatogenesis.",
author = "Ye Chu and Huddleston, {Gloria G.} and Clancy, {Andrew N.} and Harris, {Ruth Babette} and Bartness, {Timothy J.}",
year = "2010",
month = "12",
day = "1",
doi = "10.1210/en.2010-0772",
language = "English (US)",
volume = "151",
pages = "5669--5679",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "12",

}

TY - JOUR

T1 - Epididymal fat is necessary for spermatogenesis, but not testosterone production or copulatory behavior

AU - Chu, Ye

AU - Huddleston, Gloria G.

AU - Clancy, Andrew N.

AU - Harris, Ruth Babette

AU - Bartness, Timothy J.

PY - 2010/12/1

Y1 - 2010/12/1

N2 - Surgical removal of the epididymal white adipose tissue (EWAT) depot (lipectomy; EWATx) in laboratory rats or mice decreases spermatogenesis, but this phenomenal finding has not been investigated in depth. Specifically, detailed histology, neuroendocrine profiles, copulatory behavior, lipectomy of other WAT depots, rescue by autologous EWAT transplants, or tests whether this EWATx effect is due to disruption of testes innervation occurring during EWATx have not been performed. Therefore, in the first study, we performed EWATx in male Syrian hamsters and attempted to rescue spermatogenesis by transplanting the removed EWAT to the animal's subcutaneous dorsum, removed comparable or larger amounts of non-gonadal WAT [inguinal WAT (IWAT)] and conducted mating behavior tests. In a second study we conducted detailed testicular histology and assayed serum LH, FSH, and testosterone (T). In a third study, we surgically denervated the testes without removing EWAT and compared testicular histology with that of EWATx or sham surgery. We found that EWATx, but not IWATx, virtually eliminated spermatogenesis producing a marked decrease in size of the seminiferous tubule cellular lining including the Sertoli cells and spermatogonia that could not be rescued by autologous EWAT transplant to the subcutaneous dorsum. EWATx did not change serum LH or T concentrations but approximately doubled serum FSH concentrations. EWATx did not alter copulatory behavior but resulted in aspermatic ejaculate. Selective surgical testicular denervation did not affect spermatogenesis. Collectively, these results suggest the presence of a local, but currently unidentified, growth and/or nutritive factor from EWAT that promotes spermatogenesis.

AB - Surgical removal of the epididymal white adipose tissue (EWAT) depot (lipectomy; EWATx) in laboratory rats or mice decreases spermatogenesis, but this phenomenal finding has not been investigated in depth. Specifically, detailed histology, neuroendocrine profiles, copulatory behavior, lipectomy of other WAT depots, rescue by autologous EWAT transplants, or tests whether this EWATx effect is due to disruption of testes innervation occurring during EWATx have not been performed. Therefore, in the first study, we performed EWATx in male Syrian hamsters and attempted to rescue spermatogenesis by transplanting the removed EWAT to the animal's subcutaneous dorsum, removed comparable or larger amounts of non-gonadal WAT [inguinal WAT (IWAT)] and conducted mating behavior tests. In a second study we conducted detailed testicular histology and assayed serum LH, FSH, and testosterone (T). In a third study, we surgically denervated the testes without removing EWAT and compared testicular histology with that of EWATx or sham surgery. We found that EWATx, but not IWATx, virtually eliminated spermatogenesis producing a marked decrease in size of the seminiferous tubule cellular lining including the Sertoli cells and spermatogonia that could not be rescued by autologous EWAT transplant to the subcutaneous dorsum. EWATx did not change serum LH or T concentrations but approximately doubled serum FSH concentrations. EWATx did not alter copulatory behavior but resulted in aspermatic ejaculate. Selective surgical testicular denervation did not affect spermatogenesis. Collectively, these results suggest the presence of a local, but currently unidentified, growth and/or nutritive factor from EWAT that promotes spermatogenesis.

UR - http://www.scopus.com/inward/record.url?scp=78649862260&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78649862260&partnerID=8YFLogxK

U2 - 10.1210/en.2010-0772

DO - 10.1210/en.2010-0772

M3 - Article

C2 - 20881242

AN - SCOPUS:78649862260

VL - 151

SP - 5669

EP - 5679

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 12

ER -