Epigenetic mechanism underlying the development of polycystic ovary syndrome (PCOS)-like phenotypes in prenatally androgenized rhesus monkeys

Ning Xu, Soonil Kwon, David H. Abbott, David H. Geller, Daniel A. Dumesic, Ricardo Azziz, Xiuqing Guo, Mark O. Goodarzi

Research output: Contribution to journalArticle

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Abstract

The pathogenesis of polycystic ovary syndrome (PCOS) is poorly understood. PCOS-like phenotypes are produced by prenatal androgenization (PA) of female rhesus monkeys. We hypothesize that perturbation of the epigenome, through altered DNA methylation, is one of the mechanisms whereby PA reprograms monkeys to develop PCOS. Infant and adult visceral adipose tissues (VAT) harvested from 15 PA and 10 control monkeys were studied. Bisulfite treated samples were subjected to genome-wide CpG methylation analysis, designed to simultaneously measure methylation levels at 27,578 CpG sites. Analysis was carried out using Bayesian Classification with Singular Value Decomposition (BCSVD), testing all probes simultaneously in a single test. Stringent criteria were then applied to filter out invalid probes due to sequence dissimilarities between human probes and monkey DNA, and then mapped to the rhesus genome. This yielded differentially methylated loci between PA and control monkeys, 163 in infant VAT, and 325 in adult VAT (BCSVD P<0.05). Among these two sets of genes, we identified several significant pathways, including the antiproliferative role of TOB in T cell signaling and transforming growth factor-β (TGF-β) signaling. Our results suggest PA may modify DNA methylation patterns in both infant and adult VAT. This pilot study suggests that excess fetal androgen exposure in female nonhuman primates may predispose to PCOS via alteration of the epigenome, providing a novel avenue to understand PCOS in humans.

Original languageEnglish (US)
Article numbere27286
JournalPLoS One
Volume6
Issue number11
DOIs
StatePublished - Nov 4 2011

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polycystic ovary syndrome
masculinization
Virilism
Polycystic Ovary Syndrome
Macaca mulatta
Epigenomics
epigenetics
Intra-Abdominal Fat
adipose tissue
Haplorhini
monkeys
Tissue
Phenotype
phenotype
Methylation
Genes
Singular value decomposition
DNA methylation
DNA Methylation
methylation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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Epigenetic mechanism underlying the development of polycystic ovary syndrome (PCOS)-like phenotypes in prenatally androgenized rhesus monkeys. / Xu, Ning; Kwon, Soonil; Abbott, David H.; Geller, David H.; Dumesic, Daniel A.; Azziz, Ricardo; Guo, Xiuqing; Goodarzi, Mark O.

In: PLoS One, Vol. 6, No. 11, e27286, 04.11.2011.

Research output: Contribution to journalArticle

Xu, Ning ; Kwon, Soonil ; Abbott, David H. ; Geller, David H. ; Dumesic, Daniel A. ; Azziz, Ricardo ; Guo, Xiuqing ; Goodarzi, Mark O. / Epigenetic mechanism underlying the development of polycystic ovary syndrome (PCOS)-like phenotypes in prenatally androgenized rhesus monkeys. In: PLoS One. 2011 ; Vol. 6, No. 11.
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