Equine cytomegalovirus: Structural proteins of virions and nucleocapsids

Gretchen B Caughman, John Staczek, Dennis J. O'Callaghan

Research output: Contribution to journalArticle

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Abstract

Enveloped virions and nucleocapsids of equine cytomegalovirus (ECMV; equine herpesvirus type 2) have been purified from the supernatants and the nuclear extracts of infected rabbit kidney (RK) cells, respectively, and their structural protein compositions have been analyzed. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis revealed that ECMV nucleocapsids were composed of nine proteins (average molecular weights = 148K, 52K, 49.5K, 46K, 43.5K, 38.5K, 27K, 20K, and 18K), which together constituted 89% of the total nucleocapsid protein on the basis of incorporated 3H-labeled amino acids. The 148K protein comprised 47.3% of the total protein and thus appeared to be similar in molecular weight and proportional composition to the major capsid proteins of other herpesviruses. Purified virions were composed of 37 proteins whose average molecular weights ranged from 14K to greater than 200K. Three intense glycoprotein bands (83K, 78K, and 73.5K) as well as four less intensely labeled glycoproteins were detected in [3H]glucosamine-labeled virion preparations. At least 14 structural proteins were readily detected in extracts of infected cells which had been [3S)methionine labeled late in infection, and 11 of these were immunoprecipitated by rabbit antiserum against purified virions. The protein composition of ECMV differs substantially from those of equine herpesvirus type 1 and type 3 as well as from those of other herpesviruses.

Original languageEnglish (US)
Pages (from-to)184-195
Number of pages12
JournalVirology
Volume134
Issue number1
DOIs
StatePublished - Apr 15 1984
Externally publishedYes

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Nucleocapsid Proteins
Cytomegalovirus
Virion
Horses
Proteins
Nucleocapsid
Molecular Weight
Herpesviridae
Equid Herpesvirus 3
Glycoproteins
Rhadinovirus
Equid Herpesvirus 1
Rabbits
Glucosamine
Capsid Proteins
Cell Extracts
Sodium Dodecyl Sulfate
Methionine
Immune Sera
Polyacrylamide Gel Electrophoresis

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Equine cytomegalovirus : Structural proteins of virions and nucleocapsids. / Caughman, Gretchen B; Staczek, John; O'Callaghan, Dennis J.

In: Virology, Vol. 134, No. 1, 15.04.1984, p. 184-195.

Research output: Contribution to journalArticle

Caughman, Gretchen B ; Staczek, John ; O'Callaghan, Dennis J. / Equine cytomegalovirus : Structural proteins of virions and nucleocapsids. In: Virology. 1984 ; Vol. 134, No. 1. pp. 184-195.
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