Abstract
EHV-1 polypeptide synthesis was examined in productively infected rabbit kidney and hamster embryo cells. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analyses of extracts from [35S]methionine- and 3H-amino acid-labeled-infected and mock-infected cultures revealed the presence of 30 infected cell-specific polypeptides (ICPs) which ranged in apparent molecular weights from 16.5K to 213K. Twenty-two of these ICPs comigrated with virion structural proteins. Four ICPs (203K, 176K, 151K, 129K) were detected in extracts of infected cultures labeled in the presence or absence of actinomycin D (Act D) immediately after release from a 4-hr treatment with cycloheximide (CH). These polypeptides, which were designated as EHV-1 immediate early (α) ICPs, were not detected in unblocked (non-CH-treated) infected cells. The most abundant ICP was a 31.5K nonstructural protein which, in addition to a 74K protein, was detected in unblocked infected cells at 2-3 hr postinfection. These proteins appeared to be regulated as early (β) ICPs, since neither protein was observed in Act D-treated cultures released from CH block. Twelve ICPs were classified as late (γ) polypeptides on the basis of their reduced synthesis in cultures in which viral DNA replication was inhibited by phosphonoacetic acid. All but one (40K) of these late ICPs corresponded to virion structural proteins.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 49-61 |
| Number of pages | 13 |
| Journal | Virology |
| Volume | 145 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 1985 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Virology
Cite this
Equine herpesvirus type 1 infected cell polypeptides : Evidence for immediate early/early/late regulation of viral gene expression. / Caughman, Gretchen B; Staczek, John; O'Callaghan, Dennis J.
In: Virology, Vol. 145, No. 1, 01.01.1985, p. 49-61.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Equine herpesvirus type 1 infected cell polypeptides
T2 - Evidence for immediate early/early/late regulation of viral gene expression
AU - Caughman, Gretchen B
AU - Staczek, John
AU - O'Callaghan, Dennis J.
PY - 1985/1/1
Y1 - 1985/1/1
N2 - EHV-1 polypeptide synthesis was examined in productively infected rabbit kidney and hamster embryo cells. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analyses of extracts from [35S]methionine- and 3H-amino acid-labeled-infected and mock-infected cultures revealed the presence of 30 infected cell-specific polypeptides (ICPs) which ranged in apparent molecular weights from 16.5K to 213K. Twenty-two of these ICPs comigrated with virion structural proteins. Four ICPs (203K, 176K, 151K, 129K) were detected in extracts of infected cultures labeled in the presence or absence of actinomycin D (Act D) immediately after release from a 4-hr treatment with cycloheximide (CH). These polypeptides, which were designated as EHV-1 immediate early (α) ICPs, were not detected in unblocked (non-CH-treated) infected cells. The most abundant ICP was a 31.5K nonstructural protein which, in addition to a 74K protein, was detected in unblocked infected cells at 2-3 hr postinfection. These proteins appeared to be regulated as early (β) ICPs, since neither protein was observed in Act D-treated cultures released from CH block. Twelve ICPs were classified as late (γ) polypeptides on the basis of their reduced synthesis in cultures in which viral DNA replication was inhibited by phosphonoacetic acid. All but one (40K) of these late ICPs corresponded to virion structural proteins.
AB - EHV-1 polypeptide synthesis was examined in productively infected rabbit kidney and hamster embryo cells. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analyses of extracts from [35S]methionine- and 3H-amino acid-labeled-infected and mock-infected cultures revealed the presence of 30 infected cell-specific polypeptides (ICPs) which ranged in apparent molecular weights from 16.5K to 213K. Twenty-two of these ICPs comigrated with virion structural proteins. Four ICPs (203K, 176K, 151K, 129K) were detected in extracts of infected cultures labeled in the presence or absence of actinomycin D (Act D) immediately after release from a 4-hr treatment with cycloheximide (CH). These polypeptides, which were designated as EHV-1 immediate early (α) ICPs, were not detected in unblocked (non-CH-treated) infected cells. The most abundant ICP was a 31.5K nonstructural protein which, in addition to a 74K protein, was detected in unblocked infected cells at 2-3 hr postinfection. These proteins appeared to be regulated as early (β) ICPs, since neither protein was observed in Act D-treated cultures released from CH block. Twelve ICPs were classified as late (γ) polypeptides on the basis of their reduced synthesis in cultures in which viral DNA replication was inhibited by phosphonoacetic acid. All but one (40K) of these late ICPs corresponded to virion structural proteins.
UR - http://www.scopus.com/inward/record.url?scp=0022417097&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022417097&partnerID=8YFLogxK
U2 - 10.1016/0042-6822(85)90200-4
DO - 10.1016/0042-6822(85)90200-4
M3 - Article
C2 - 2990102
AN - SCOPUS:0022417097
VL - 145
SP - 49
EP - 61
JO - Virology
JF - Virology
SN - 0042-6822
IS - 1
ER -