Abstract
Cortical lamination is crucial for the assembly of cerebellar circuitry. In this process, granule neurons (GNs) migrate along Bergmann glia (BG), which are specialized astroglial cells, from the external granule layer to the internal granule layer. However, the molecular mechanisms underlying BG development are not well understood. Here, we show that GFAP::Cre;Erbb3F/F mice, which lack Erbb3 in both radial glia and neurons, exhibit impairments in balance and motor coordination. Cerebellar lamination is aberrant, with misplaced Purkinje neurons and GN clusters. These phenotypes were not observed in Math1::CreERT2;Erbb3F/F mice, where the Erbb3 gene was deleted in GNs, suggesting involvement of non-neuronal Erbb3 in cerebellar lamination. Mechanistic studies indicate that ERBB3 is crucial for the proliferation of BG, which are required for GN migration. These observations identify a crucial role for ERBB3 in cerebellar lamination and reveal a novel mechanism that regulates BG development.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 522-532 |
| Number of pages | 11 |
| Journal | Development (Cambridge) |
| Volume | 142 |
| Issue number | 3 |
| DOIs | |
| State | Published - Feb 1 2015 |
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Keywords
- Bergmann glia
- Cerebellum
- ERBB3
- Mouse
ASJC Scopus subject areas
- Molecular Biology
- Developmental Biology
Cite this
Erbb3-mediated regulation of bergmann glia proliferation in cerebellar lamination. / Sathyamurthy, Anupama; Yin, Dong Min; Barik, Arnab; Shen, Chengyong; Bean, Jonathan C.; Figueiredo, Dwight; She, Jin Xiong; Xiong, Wen Cheng; Mei, Lin.
In: Development (Cambridge), Vol. 142, No. 3, 01.02.2015, p. 522-532.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Erbb3-mediated regulation of bergmann glia proliferation in cerebellar lamination
AU - Sathyamurthy, Anupama
AU - Yin, Dong Min
AU - Barik, Arnab
AU - Shen, Chengyong
AU - Bean, Jonathan C.
AU - Figueiredo, Dwight
AU - She, Jin Xiong
AU - Xiong, Wen Cheng
AU - Mei, Lin
PY - 2015/2/1
Y1 - 2015/2/1
N2 - Cortical lamination is crucial for the assembly of cerebellar circuitry. In this process, granule neurons (GNs) migrate along Bergmann glia (BG), which are specialized astroglial cells, from the external granule layer to the internal granule layer. However, the molecular mechanisms underlying BG development are not well understood. Here, we show that GFAP::Cre;Erbb3F/F mice, which lack Erbb3 in both radial glia and neurons, exhibit impairments in balance and motor coordination. Cerebellar lamination is aberrant, with misplaced Purkinje neurons and GN clusters. These phenotypes were not observed in Math1::CreERT2;Erbb3F/F mice, where the Erbb3 gene was deleted in GNs, suggesting involvement of non-neuronal Erbb3 in cerebellar lamination. Mechanistic studies indicate that ERBB3 is crucial for the proliferation of BG, which are required for GN migration. These observations identify a crucial role for ERBB3 in cerebellar lamination and reveal a novel mechanism that regulates BG development.
AB - Cortical lamination is crucial for the assembly of cerebellar circuitry. In this process, granule neurons (GNs) migrate along Bergmann glia (BG), which are specialized astroglial cells, from the external granule layer to the internal granule layer. However, the molecular mechanisms underlying BG development are not well understood. Here, we show that GFAP::Cre;Erbb3F/F mice, which lack Erbb3 in both radial glia and neurons, exhibit impairments in balance and motor coordination. Cerebellar lamination is aberrant, with misplaced Purkinje neurons and GN clusters. These phenotypes were not observed in Math1::CreERT2;Erbb3F/F mice, where the Erbb3 gene was deleted in GNs, suggesting involvement of non-neuronal Erbb3 in cerebellar lamination. Mechanistic studies indicate that ERBB3 is crucial for the proliferation of BG, which are required for GN migration. These observations identify a crucial role for ERBB3 in cerebellar lamination and reveal a novel mechanism that regulates BG development.
KW - Bergmann glia
KW - Cerebellum
KW - ERBB3
KW - Mouse
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UR - http://www.scopus.com/inward/citedby.url?scp=84921533676&partnerID=8YFLogxK
U2 - 10.1242/dev.115931
DO - 10.1242/dev.115931
M3 - Article
C2 - 25564653
AN - SCOPUS:84921533676
VL - 142
SP - 522
EP - 532
JO - Development (Cambridge)
JF - Development (Cambridge)
SN - 0950-1991
IS - 3
ER -