Erbium, chromium: Yttrium-scandium-gallium-garnet laser effectively ablates single-species biofilms on titanium disks without detectable surface damage

Jason M. Strever, Jaebum Lee, William Ealick, Mark E. Peacock, Daniel Shelby, Cristiano Susin, Donald Mettenberg, Ahmed El-Awady, Frederick Rueggeberg, Christopher W Cutler

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Increasing evidence implicates biofilms, consisting of species such as Porphyromonas gingivalis (Pg), in the etiology of peri-implantitis. Multiple approaches to ablate biofilms on failing implants have been proposed and include use of lasers, most recently the erbium, chromium:yttriumscandium-gallium-garnet (Er,Cr:YSGG) laser. The purpose of this study is to establish an in vitro single-species biofilm model on implant surfaces and determine power settings of the Er,Cr:YSGG laser that remove biofilm without causing physical damage to disks. Methods: Single-species biofilms consisting of Pg strain 381 were grown on titanium disks, including: 1) sandblasted, large-grit, acid-etched (SLA); 2) calcium phosphate nanocoated (CaP); 3) anodized; or 4) machined surfaces. Power settings from 0 to 1.5 W using an Er,Cr:YSGG laser equipped with radial firing tip were used. Biofilm formation/removal was quantitated using confocal and scanning electron microscopy. Surface changes in temperature, microroughness, and water contact angle were analyzed. Results: Results show confluent Pg biofilm coating all disk surfaces. The laser removed biofilms from all surfaces, with CaP and SLA surfaces requiring power setting of 1.0 to 1.5 W for ablation of bacteria coating the disks. Within this power range, and with water spray, there were no changes in surface temperature, surface roughness, or contact angle on any surfaces tested. Conclusion: The Er,Cr:YSGG laser with radial firing tip and water spray was able to effectively ablate 95% of biofilm on all types of tested titanium surfaces, using clinically relevant power settings, without causing measurable physical changes to surfaces. J Periodontol 2017;88:484-492.

Original languageEnglish (US)
Pages (from-to)484-492
Number of pages9
JournalJournal of periodontology
Volume88
Issue number5
DOIs
StatePublished - May 1 2017

Fingerprint

Erbium
Solid-State Lasers
Chromium
Biofilms
Titanium
Gallium
Lasers
Porphyromonas gingivalis
Water
Peri-Implantitis
Temperature
Acids
Electron Scanning Microscopy
Bacteria

Keywords

  • Biofilms
  • Decontamination
  • Lasers
  • Porphyromonas gingivalis
  • Titanium

ASJC Scopus subject areas

  • Periodontics

Cite this

Erbium, chromium : Yttrium-scandium-gallium-garnet laser effectively ablates single-species biofilms on titanium disks without detectable surface damage. / Strever, Jason M.; Lee, Jaebum; Ealick, William; Peacock, Mark E.; Shelby, Daniel; Susin, Cristiano; Mettenberg, Donald; El-Awady, Ahmed; Rueggeberg, Frederick; Cutler, Christopher W.

In: Journal of periodontology, Vol. 88, No. 5, 01.05.2017, p. 484-492.

Research output: Contribution to journalArticle

Strever, Jason M. ; Lee, Jaebum ; Ealick, William ; Peacock, Mark E. ; Shelby, Daniel ; Susin, Cristiano ; Mettenberg, Donald ; El-Awady, Ahmed ; Rueggeberg, Frederick ; Cutler, Christopher W. / Erbium, chromium : Yttrium-scandium-gallium-garnet laser effectively ablates single-species biofilms on titanium disks without detectable surface damage. In: Journal of periodontology. 2017 ; Vol. 88, No. 5. pp. 484-492.
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abstract = "Background: Increasing evidence implicates biofilms, consisting of species such as Porphyromonas gingivalis (Pg), in the etiology of peri-implantitis. Multiple approaches to ablate biofilms on failing implants have been proposed and include use of lasers, most recently the erbium, chromium:yttriumscandium-gallium-garnet (Er,Cr:YSGG) laser. The purpose of this study is to establish an in vitro single-species biofilm model on implant surfaces and determine power settings of the Er,Cr:YSGG laser that remove biofilm without causing physical damage to disks. Methods: Single-species biofilms consisting of Pg strain 381 were grown on titanium disks, including: 1) sandblasted, large-grit, acid-etched (SLA); 2) calcium phosphate nanocoated (CaP); 3) anodized; or 4) machined surfaces. Power settings from 0 to 1.5 W using an Er,Cr:YSGG laser equipped with radial firing tip were used. Biofilm formation/removal was quantitated using confocal and scanning electron microscopy. Surface changes in temperature, microroughness, and water contact angle were analyzed. Results: Results show confluent Pg biofilm coating all disk surfaces. The laser removed biofilms from all surfaces, with CaP and SLA surfaces requiring power setting of 1.0 to 1.5 W for ablation of bacteria coating the disks. Within this power range, and with water spray, there were no changes in surface temperature, surface roughness, or contact angle on any surfaces tested. Conclusion: The Er,Cr:YSGG laser with radial firing tip and water spray was able to effectively ablate 95{\%} of biofilm on all types of tested titanium surfaces, using clinically relevant power settings, without causing measurable physical changes to surfaces. J Periodontol 2017;88:484-492.",
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AU - Lee, Jaebum

AU - Ealick, William

AU - Peacock, Mark E.

AU - Shelby, Daniel

AU - Susin, Cristiano

AU - Mettenberg, Donald

AU - El-Awady, Ahmed

AU - Rueggeberg, Frederick

AU - Cutler, Christopher W

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AB - Background: Increasing evidence implicates biofilms, consisting of species such as Porphyromonas gingivalis (Pg), in the etiology of peri-implantitis. Multiple approaches to ablate biofilms on failing implants have been proposed and include use of lasers, most recently the erbium, chromium:yttriumscandium-gallium-garnet (Er,Cr:YSGG) laser. The purpose of this study is to establish an in vitro single-species biofilm model on implant surfaces and determine power settings of the Er,Cr:YSGG laser that remove biofilm without causing physical damage to disks. Methods: Single-species biofilms consisting of Pg strain 381 were grown on titanium disks, including: 1) sandblasted, large-grit, acid-etched (SLA); 2) calcium phosphate nanocoated (CaP); 3) anodized; or 4) machined surfaces. Power settings from 0 to 1.5 W using an Er,Cr:YSGG laser equipped with radial firing tip were used. Biofilm formation/removal was quantitated using confocal and scanning electron microscopy. Surface changes in temperature, microroughness, and water contact angle were analyzed. Results: Results show confluent Pg biofilm coating all disk surfaces. The laser removed biofilms from all surfaces, with CaP and SLA surfaces requiring power setting of 1.0 to 1.5 W for ablation of bacteria coating the disks. Within this power range, and with water spray, there were no changes in surface temperature, surface roughness, or contact angle on any surfaces tested. Conclusion: The Er,Cr:YSGG laser with radial firing tip and water spray was able to effectively ablate 95% of biofilm on all types of tested titanium surfaces, using clinically relevant power settings, without causing measurable physical changes to surfaces. J Periodontol 2017;88:484-492.

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