Erectile function in two-kidney, one-clip hypertensive rats is maintained by a potential increase in nitric oxide production

A. Elizabeth Linder, Anne M. Dorrance, Thomas M. Mills, R. Clinton Webb, Romulo Leite

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8 Scopus citations

Abstract

Introduction. Hypertension is closely associated with erectile dysfunction (ED) as it has been observed in many experimental models of hypertension. Additionally, epidemiological studies show that approximately a third of hypertensive patients have ED. Aim. To test the hypothesis that the two-kidney, one-clip (2K-1C) rat model of hypertension displays normal erectile function due to increased nitric oxide (NO) production in the penis. Methods. Ganglionic-induced increase in intracavernosal pressure (ICP)/mean arterial pressure (MAP) ratio was used as an index of erectile function in 2K-1C and in normotensive sham-operated (SHAM) anesthetized rats. Cavernosal strips from hypertensive and normotensive rats were used for isometric tension measurement. The contraction induced by alpha-adrenergic agonist phenylephrine and the relaxation induced by the NO donor sodium nitroprusside (SNP) and by the Rho-kinase inhibitor Y-27632 were performed in the absence and in the presence of the NO synthase inhibitor Nω-nitro-L-arginine (L-NNA). Results. Changes in ICP/MAP induced by ganglionic stimulation were not different between 2K-1C and SHAM rats. The contractile response induced by phenylephrine as well as the relaxation induced by SNP or the Y-27632 were similar in cavernosal strips from both groups. However, in the presence of L-NNA, the relaxation induced by Y-27632 was significantly impaired in 2K-1C compared to SHAM. Conclusions. These data suggest that hypertension and ED could be dissociated from high levels of blood pressure in some animal models of hypertension. Erectile function in 2K-1C hypertensive rats is maintained in spite of the increased Rho-kinase activity by increased NO signaling.

Original languageEnglish (US)
Pages (from-to)279-285
Number of pages7
JournalJournal of Sexual Medicine
Volume6
Issue numberSUPPL. 3
DOIs
StatePublished - Jan 1 2009

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Keywords

  • Erectile function
  • Nitric oxide
  • One-clip hypertension
  • Rho-kinase
  • Two-kidney

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology
  • Urology

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