Abstract
Fetal hemoglobin (HbF) induction is an effective approach to improve clinical symptoms in sickle cell disease. Understanding molecular mechanisms for gamma-gene re-activation will aid efforts to design lead compounds. A potential inhibitory role for the extracellular signal-regulated kinase (ERK) mitogen-activated protein kinase (MAPK) pathway in gamma-gene expression has been suggested recently. Therefore, we determined the ability of U0126, a selective inhibitor of MEK1/2 the upstream activators of ERK, to re-activate gamma-globin expression. K562 stable lines over-expressing constitutively active MEK1 were established. A significant increase in ERK phosphorylation was observed and gamma-gene expression was silenced concomitantly, however U0126 attenuated this effect. Studies in human erythroid progenitors confirmed the ability of U0126 to induce HbF. Cellular mechanisms for the inhibitory role of ERK signaling in drug-mediated HbF induction will be discussed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 215-227 |
| Number of pages | 13 |
| Journal | Cellular and Molecular Biology |
| Volume | 51 |
| Issue number | 2 |
| State | Published - Sep 5 2005 |
| Externally published | Yes |
Keywords
- ERK
- Fetal hemoglobin
- Gamma-globin
- Sickle cell disease
- U0126
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology
