Essential Requirement for Both hsf1 and hsf2 Transcriptional Activity in Spermatogenesis and Male Fertility

Guanghu Wang, Zhekang Ying, Xiongjie Jin, Naxin Tu, Yan Zhang, Michele Phillips, Dimitrios Moskofidis, Nahid F Mivechi

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Heat shock factors (Hsfs) are major transactivators of heat shock proteins but are also involved in regulation of other genes active in embryonic development. High expression levels of Hsfs in mouse testis during development suggest a role for these factors in spermatogenesis, a cyclic process of spermatogonia cell-differentiation into mature spermatozoa. In contrast to hsf1-/- mice, which exhibit normal spermatogenesis, targeted disruption of hsf2 results in reduced testicular size but only a small impairment in male fertility. We show here that disruption of both hsf1 and hsf2 results in a more severe phenotype associated with male sterility due to severe defects in spermatogenesis. Earliest defects observed are the reduced number of germ cells in juvenile mice and germ cells that enter the meiotic prophase fail to progress beyond the pachytene stage. This was associated with a reduction or absence of transcription of genes critically involved in spermatogenesis. The findings suggest that additive or synergistic transcriptional activity of both hsf1 and hsf2 is required for normal mammalian spermatogenesis and male fertility.

Original languageEnglish (US)
Pages (from-to)66-80
Number of pages15
JournalGenesis
Volume38
Issue number2
DOIs
StatePublished - Feb 1 2004

Fingerprint

Spermatogenesis
Fertility
Germ Cells
Pachytene Stage
Prophase
Spermatogonia
Trans-Activators
Male Infertility
Heat-Shock Proteins
Genes
Embryonic Development
Spermatozoa
Testis
Cell Differentiation
Shock
Hot Temperature
Phenotype

Keywords

  • Gene-array analyses
  • Heat shock proteins
  • Heat shock transcription factors
  • Infertility
  • Knockout mice

ASJC Scopus subject areas

  • Genetics

Cite this

Essential Requirement for Both hsf1 and hsf2 Transcriptional Activity in Spermatogenesis and Male Fertility. / Wang, Guanghu; Ying, Zhekang; Jin, Xiongjie; Tu, Naxin; Zhang, Yan; Phillips, Michele; Moskofidis, Dimitrios; Mivechi, Nahid F.

In: Genesis, Vol. 38, No. 2, 01.02.2004, p. 66-80.

Research output: Contribution to journalArticle

Wang, Guanghu ; Ying, Zhekang ; Jin, Xiongjie ; Tu, Naxin ; Zhang, Yan ; Phillips, Michele ; Moskofidis, Dimitrios ; Mivechi, Nahid F. / Essential Requirement for Both hsf1 and hsf2 Transcriptional Activity in Spermatogenesis and Male Fertility. In: Genesis. 2004 ; Vol. 38, No. 2. pp. 66-80.
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