Estradiol relaxes rat aorta via endothelium-dependent and -independent mechanisms

Gamal Abou-Mohamed, Ahmed Abdelrazik Elmarakby, Gerald O. Carrier, John D. Catravas, Robert William Caldwell, Richard E. White

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The effects of estrogen on arterial function are heterogeneous with respect to vessel and/or species. We have investigated 17β-estradiol-induced relaxation in isolated rat aorta with regard to the role of the vascular endothelium and ionic mechanisms. Estrogen induced a concentration-dependent relaxation of 46.5 ± 7.9% and 70.1 ± 12.2% (10-8 and 10-7 M), which was reduced by endothelial denudation. Furthermore, L-nitroarginine methyl ester completely abrogated this effect; however, estradiol did not relax KCl-contracted rings. Tetraethyl ammonium (1 mmol/l) completely blocked estradiol-induced relaxation. Estradiol increased [cGMP] in isolated aortic rings via NO, but did not significantly affect NOS activity in endothelial cells. Thus, estrogen can relax rat aorta in vitro via both endothelium-dependent and -independent mechanisms involving the NO/cGMP and potassium channel signaling system.

Original languageEnglish (US)
Pages (from-to)20-26
Number of pages7
JournalPharmacology
Volume69
Issue number1
DOIs
StatePublished - Aug 11 2003

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Endothelium
Aorta
Estradiol
Estrogens
Nitroarginine
Potassium Channels
Vascular Endothelium
Ammonium Compounds
Esters
Endothelial Cells

Keywords

  • Aorta
  • Estrogen
  • Nitric oxide
  • Potassium channel

ASJC Scopus subject areas

  • Pharmacology

Cite this

Estradiol relaxes rat aorta via endothelium-dependent and -independent mechanisms. / Abou-Mohamed, Gamal; Elmarakby, Ahmed Abdelrazik; Carrier, Gerald O.; Catravas, John D.; Caldwell, Robert William; White, Richard E.

In: Pharmacology, Vol. 69, No. 1, 11.08.2003, p. 20-26.

Research output: Contribution to journalArticle

Abou-Mohamed, Gamal ; Elmarakby, Ahmed Abdelrazik ; Carrier, Gerald O. ; Catravas, John D. ; Caldwell, Robert William ; White, Richard E. / Estradiol relaxes rat aorta via endothelium-dependent and -independent mechanisms. In: Pharmacology. 2003 ; Vol. 69, No. 1. pp. 20-26.
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