TY - JOUR
T1 - Estrogenicity of isoflavones on human endometrial stromal and glandular cells
AU - Kayisli, Umit A.
AU - Aksu, Cinar Ahmet H.
AU - Berkkanoglu, Murat
AU - Arici, Aydin
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2002/12/1
Y1 - 2002/12/1
N2 - Endometrium consists of different cell populations such as epithelial and stromal cells and is mainly regulated by sex steroids. Isoflavones are plant-derived estrogenic compounds that have estrogenic and antiestrogenic properties in a cell-specific manner. We hypothesized that one of the potential health benefits of isoflavones may be their ability to regulate endometrial cell function. The present study was conducted to assess estrogenic and/or antiestrogenic effects of isoflavones (genistein, genistin, daidzein, and daidzin) in cultured human endometrial stromal and glandular (Ishikawa) cells by MTT colorimetric cell proliferation assay, proliferating cell nuclear antigen expression, and alkaline phosphatase activity assays. Experiments were performed in a time- (24-96 h) and concentration-dependent (10-12 to 10-5 M) manner. All isoflavones used in the present study induced endometrial stromal and Ishikawa cell proliferation when compared with control (vehicle) group in a time- (at 48 h and afterward) and concentration-dependent manner (at 10-8 M and above) (P < 0.05). However, isoflavones (at 10-8 and above concentrations) were also antiestrogenic when combined with estradiol (E2) (P < 0.05). The isoflavones revealed a weak estrogenic activity (39-67% less than E2) as assessed by alkaline phosphatase activity (P < 0.05), but when administered together with E2, they antagonized estrogen induced alkaline phosphatase activity by 36-89% (P < 0.05). We conclude that, although isoflavones alone have weak estrogenic effects on endometrial stromal and glandular cells, in the presence of E2 they act as antiestrogens.
AB - Endometrium consists of different cell populations such as epithelial and stromal cells and is mainly regulated by sex steroids. Isoflavones are plant-derived estrogenic compounds that have estrogenic and antiestrogenic properties in a cell-specific manner. We hypothesized that one of the potential health benefits of isoflavones may be their ability to regulate endometrial cell function. The present study was conducted to assess estrogenic and/or antiestrogenic effects of isoflavones (genistein, genistin, daidzein, and daidzin) in cultured human endometrial stromal and glandular (Ishikawa) cells by MTT colorimetric cell proliferation assay, proliferating cell nuclear antigen expression, and alkaline phosphatase activity assays. Experiments were performed in a time- (24-96 h) and concentration-dependent (10-12 to 10-5 M) manner. All isoflavones used in the present study induced endometrial stromal and Ishikawa cell proliferation when compared with control (vehicle) group in a time- (at 48 h and afterward) and concentration-dependent manner (at 10-8 M and above) (P < 0.05). However, isoflavones (at 10-8 and above concentrations) were also antiestrogenic when combined with estradiol (E2) (P < 0.05). The isoflavones revealed a weak estrogenic activity (39-67% less than E2) as assessed by alkaline phosphatase activity (P < 0.05), but when administered together with E2, they antagonized estrogen induced alkaline phosphatase activity by 36-89% (P < 0.05). We conclude that, although isoflavones alone have weak estrogenic effects on endometrial stromal and glandular cells, in the presence of E2 they act as antiestrogens.
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U2 - 10.1210/jc.2002-020716
DO - 10.1210/jc.2002-020716
M3 - Article
C2 - 12466350
AN - SCOPUS:0036929941
VL - 87
SP - 5539
EP - 5544
JO - Journal of Clinical Endocrinology and Metabolism
JF - Journal of Clinical Endocrinology and Metabolism
SN - 0021-972X
IS - 12
ER -