Estrogenicity of isoflavones on human endometrial stromal and glandular cells

Umit A. Kayisli, Cinar Ahmet H. Aksu, Murat Berkkanoglu, Aydin Arici

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Endometrium consists of different cell populations such as epithelial and stromal cells and is mainly regulated by sex steroids. Isoflavones are plant-derived estrogenic compounds that have estrogenic and antiestrogenic properties in a cell-specific manner. We hypothesized that one of the potential health benefits of isoflavones may be their ability to regulate endometrial cell function. The present study was conducted to assess estrogenic and/or antiestrogenic effects of isoflavones (genistein, genistin, daidzein, and daidzin) in cultured human endometrial stromal and glandular (Ishikawa) cells by MTT colorimetric cell proliferation assay, proliferating cell nuclear antigen expression, and alkaline phosphatase activity assays. Experiments were performed in a time- (24-96 h) and concentration-dependent (10-12 to 10-5 M) manner. All isoflavones used in the present study induced endometrial stromal and Ishikawa cell proliferation when compared with control (vehicle) group in a time- (at 48 h and afterward) and concentration-dependent manner (at 10-8 M and above) (P < 0.05). However, isoflavones (at 10-8 and above concentrations) were also antiestrogenic when combined with estradiol (E2) (P < 0.05). The isoflavones revealed a weak estrogenic activity (39-67% less than E2) as assessed by alkaline phosphatase activity (P < 0.05), but when administered together with E2, they antagonized estrogen induced alkaline phosphatase activity by 36-89% (P < 0.05). We conclude that, although isoflavones alone have weak estrogenic effects on endometrial stromal and glandular cells, in the presence of E2 they act as antiestrogens.

Original languageEnglish (US)
Pages (from-to)5539-5544
Number of pages6
JournalJournal of Clinical Endocrinology and Metabolism
Volume87
Issue number12
DOIs
StatePublished - Dec 1 2002

Fingerprint

Isoflavones
Stromal Cells
Alkaline Phosphatase
Estrogens
Cell proliferation
Assays
Cell Proliferation
Estrogen Receptor Modulators
Genistein
Proliferating Cell Nuclear Antigen
Phytochemicals
Insurance Benefits
Endometrium
Estradiol
Epithelial Cells
Steroids
Cells
Health
Control Groups
Population

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Estrogenicity of isoflavones on human endometrial stromal and glandular cells. / Kayisli, Umit A.; Aksu, Cinar Ahmet H.; Berkkanoglu, Murat; Arici, Aydin.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 87, No. 12, 01.12.2002, p. 5539-5544.

Research output: Contribution to journalArticle

Kayisli, Umit A. ; Aksu, Cinar Ahmet H. ; Berkkanoglu, Murat ; Arici, Aydin. / Estrogenicity of isoflavones on human endometrial stromal and glandular cells. In: Journal of Clinical Endocrinology and Metabolism. 2002 ; Vol. 87, No. 12. pp. 5539-5544.
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