Ethanol-wet bonding technique: Clinical versus laboratory findings

Eunice Kuhn, Patrícia Farhat, Ana Paula Teitelbaum, Alexandra Mena-Serrano, Alessandro D. Loguercio, Alessandra Reis, David H. Pashley

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Abstract Objectives This study evaluated the microtensile bond strength (μTBS) and nanoleakage (NL) of dentin bonded interfaces produced with ethanol-wet and water-wet bonding protocols under clinical and laboratory conditions. Methods The sample was composed of forty primary second molars in advanced exfoliation process. Occlusal cavities were prepared leaving a flat dentin surface on the pulpal floor. In half of the teeth, the water-wet protocol was followed using a three-step etch-and-rinse adhesive. In the other half, dentin was dehydrated with ascending ethanol solutions (50%, 70%, 80%, 95% and 3 × 100%), 15 s each for the ethanol-bonding protocol. An experimental hydrophobic primer was used, followed by the neat adhesive application. Resin build-ups were prepared, stored for 24 h, sectioned into sticks and tested in tensile mode (0.5 mm/min). NL was performed for all groups. The μTBS and NL data were submitted to two-way ANOVA and Kruskall-Wallis tests, respectively (α = 0.05). Results Under clinical conditions, the highest μTBS was observed for the water-wet bonding while under the laboratory setting, the highest μTBS was obtained for the ethanol-wet bonding. Increased NL was observed in the water-wet bonding groups irrespective of the bonding condition. Significance The immediate benefits of the ethanol-bonding observed in the laboratory setting was not confirmed when the same protocol was performed in vivo. However, as reduced nanoleakage was seen in adhesive interfaces produced with the ethanol-wet bonding technique, suggests that the hybrid layer may be more resistant to degradation.

Original languageEnglish (US)
Article number2570
Pages (from-to)1030-1037
Number of pages8
JournalDental Materials
Volume31
Issue number9
DOIs
StatePublished - Jan 1 2015

Fingerprint

Clinical laboratories
Clinical Laboratory Techniques
Ethanol
Dentin
Adhesives
Water
Clinical Protocols
Analysis of Variance
Tooth
Analysis of variance (ANOVA)
Resins
Degradation

Keywords

  • Ethanol-wet bonding
  • In vitro
  • In vivo
  • Microtensile bond strength
  • Nanoleakage

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Kuhn, E., Farhat, P., Teitelbaum, A. P., Mena-Serrano, A., Loguercio, A. D., Reis, A., & Pashley, D. H. (2015). Ethanol-wet bonding technique: Clinical versus laboratory findings. Dental Materials, 31(9), 1030-1037. [2570]. https://doi.org/10.1016/j.dental.2015.05.010

Ethanol-wet bonding technique : Clinical versus laboratory findings. / Kuhn, Eunice; Farhat, Patrícia; Teitelbaum, Ana Paula; Mena-Serrano, Alexandra; Loguercio, Alessandro D.; Reis, Alessandra; Pashley, David H.

In: Dental Materials, Vol. 31, No. 9, 2570, 01.01.2015, p. 1030-1037.

Research output: Contribution to journalArticle

Kuhn, E, Farhat, P, Teitelbaum, AP, Mena-Serrano, A, Loguercio, AD, Reis, A & Pashley, DH 2015, 'Ethanol-wet bonding technique: Clinical versus laboratory findings', Dental Materials, vol. 31, no. 9, 2570, pp. 1030-1037. https://doi.org/10.1016/j.dental.2015.05.010
Kuhn E, Farhat P, Teitelbaum AP, Mena-Serrano A, Loguercio AD, Reis A et al. Ethanol-wet bonding technique: Clinical versus laboratory findings. Dental Materials. 2015 Jan 1;31(9):1030-1037. 2570. https://doi.org/10.1016/j.dental.2015.05.010
Kuhn, Eunice ; Farhat, Patrícia ; Teitelbaum, Ana Paula ; Mena-Serrano, Alexandra ; Loguercio, Alessandro D. ; Reis, Alessandra ; Pashley, David H. / Ethanol-wet bonding technique : Clinical versus laboratory findings. In: Dental Materials. 2015 ; Vol. 31, No. 9. pp. 1030-1037.
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N2 - Abstract Objectives This study evaluated the microtensile bond strength (μTBS) and nanoleakage (NL) of dentin bonded interfaces produced with ethanol-wet and water-wet bonding protocols under clinical and laboratory conditions. Methods The sample was composed of forty primary second molars in advanced exfoliation process. Occlusal cavities were prepared leaving a flat dentin surface on the pulpal floor. In half of the teeth, the water-wet protocol was followed using a three-step etch-and-rinse adhesive. In the other half, dentin was dehydrated with ascending ethanol solutions (50%, 70%, 80%, 95% and 3 × 100%), 15 s each for the ethanol-bonding protocol. An experimental hydrophobic primer was used, followed by the neat adhesive application. Resin build-ups were prepared, stored for 24 h, sectioned into sticks and tested in tensile mode (0.5 mm/min). NL was performed for all groups. The μTBS and NL data were submitted to two-way ANOVA and Kruskall-Wallis tests, respectively (α = 0.05). Results Under clinical conditions, the highest μTBS was observed for the water-wet bonding while under the laboratory setting, the highest μTBS was obtained for the ethanol-wet bonding. Increased NL was observed in the water-wet bonding groups irrespective of the bonding condition. Significance The immediate benefits of the ethanol-bonding observed in the laboratory setting was not confirmed when the same protocol was performed in vivo. However, as reduced nanoleakage was seen in adhesive interfaces produced with the ethanol-wet bonding technique, suggests that the hybrid layer may be more resistant to degradation.

AB - Abstract Objectives This study evaluated the microtensile bond strength (μTBS) and nanoleakage (NL) of dentin bonded interfaces produced with ethanol-wet and water-wet bonding protocols under clinical and laboratory conditions. Methods The sample was composed of forty primary second molars in advanced exfoliation process. Occlusal cavities were prepared leaving a flat dentin surface on the pulpal floor. In half of the teeth, the water-wet protocol was followed using a three-step etch-and-rinse adhesive. In the other half, dentin was dehydrated with ascending ethanol solutions (50%, 70%, 80%, 95% and 3 × 100%), 15 s each for the ethanol-bonding protocol. An experimental hydrophobic primer was used, followed by the neat adhesive application. Resin build-ups were prepared, stored for 24 h, sectioned into sticks and tested in tensile mode (0.5 mm/min). NL was performed for all groups. The μTBS and NL data were submitted to two-way ANOVA and Kruskall-Wallis tests, respectively (α = 0.05). Results Under clinical conditions, the highest μTBS was observed for the water-wet bonding while under the laboratory setting, the highest μTBS was obtained for the ethanol-wet bonding. Increased NL was observed in the water-wet bonding groups irrespective of the bonding condition. Significance The immediate benefits of the ethanol-bonding observed in the laboratory setting was not confirmed when the same protocol was performed in vivo. However, as reduced nanoleakage was seen in adhesive interfaces produced with the ethanol-wet bonding technique, suggests that the hybrid layer may be more resistant to degradation.

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