TY - JOUR
T1 - Evaluation of guggulipid and nimesulide on production of inflammatory mediators and GFAP expression in LPS stimulated rat astrocytoma, cell line (C6)
AU - Niranjan, Rituraj
AU - Kamat, Pradeep Kumar
AU - Nath, Chandishwar
AU - Shukla, Rakesh
N1 - Funding Information:
Senior Research Fellowship of Indian Council of Medical Research, New Delhi, India to RRN is gratefully acknowledged.
PY - 2010/2
Y1 - 2010/2
N2 - Aim of the study: The present study was designed to investigate effect of guggulipid, a drug developed by CDRI and nimesulide on LPS stimulated neuroinflammatory changes in rat astrocytoma cell line (C6). Materials and methods: Rat astrocytoma cells (C6) were stimulated with LPS (10μg/ml) alone and in combinations with different concentrations of guggulipid or nimesulide for 24. h of incubation. Nitrite release in culture supernatant, ROS in cells, expressions of COX-2, GFAP and TNF-α in cell lysate were estimated. Results: LPS (10 μg/ml) stimulated C6 cells to release nitrite, ROS generation, up regulated COX-2 and GFAP expressions at protein level and TNF-α at mRNA level. Both guggulipid and nimesulide significantly attenuated nitrite release, ROS generation and also down regulated expressions of COX-2, GFAP and TNF-α. Guggulipid and nimesulide per se did not have any significant effect on C6 cells. Conclusion: Results demonstrate the anti-inflammatory effect of guggulipid comparable to nimesulide which suggest potential use of guggulipid in neuroinflammation associated conditions in CNS disorders.
AB - Aim of the study: The present study was designed to investigate effect of guggulipid, a drug developed by CDRI and nimesulide on LPS stimulated neuroinflammatory changes in rat astrocytoma cell line (C6). Materials and methods: Rat astrocytoma cells (C6) were stimulated with LPS (10μg/ml) alone and in combinations with different concentrations of guggulipid or nimesulide for 24. h of incubation. Nitrite release in culture supernatant, ROS in cells, expressions of COX-2, GFAP and TNF-α in cell lysate were estimated. Results: LPS (10 μg/ml) stimulated C6 cells to release nitrite, ROS generation, up regulated COX-2 and GFAP expressions at protein level and TNF-α at mRNA level. Both guggulipid and nimesulide significantly attenuated nitrite release, ROS generation and also down regulated expressions of COX-2, GFAP and TNF-α. Guggulipid and nimesulide per se did not have any significant effect on C6 cells. Conclusion: Results demonstrate the anti-inflammatory effect of guggulipid comparable to nimesulide which suggest potential use of guggulipid in neuroinflammation associated conditions in CNS disorders.
KW - Guggulipid
KW - Lipo-polysaccharide
KW - Neuroinflammation
KW - Nimesulide
KW - Rat astrocytoma cell line (C6)
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U2 - 10.1016/j.jep.2009.12.012
DO - 10.1016/j.jep.2009.12.012
M3 - Article
C2 - 20018235
AN - SCOPUS:76349084060
SN - 0378-8741
VL - 127
SP - 625
EP - 630
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
IS - 3
ER -