Evaluation of implants coated with recombinant human bone morphogenetic protein-2 and vacuum-dried using the critical-size supraalveolar peri-implant defect model in dogs

John F. Decker, Jaebum Lee, Carlo Alberto COrtella, Giuseppe Polimeni, Michael D. Rohrer, John M. Wozney, Jan Hall, Cristiano Susin, Ulf M E Wikesjö

Research output: Contribution to journalArticle

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Abstract

Background: Endosseous implants coated with recombinant human bone morphogenetic protein-2 (rhBMP-2) in a laboratory bench setting and air-dried induce relevant bone formation but also resident bone remodeling. Thus, the objective of this study is to evaluate the effect of implants fully or partially coated with rhBMP-2 and vacuum-dried using an industrial process on local bone formation and resident bone remodeling. Methods: Twelve male adult Hound Labrador mongrel dogs were used. Critical-size, supraalveolar, periimplant defects received titanium porous oxide surface implants coated in their most coronal aspect with rhBMP-2 (coronal-load, six animals), or by immersion of the entire implant in a rhBMP-2 solution (soakload, six animals) for a total of 30 μg rhBMP-2 per implant. All implants were vacuum-dried. The animals were sacrificed at 8 weeks for histometric evaluation. Results: Clinical healing was unremarkable. Bone formation was not significantly affected by the rhBMP- 2 application protocol. New bone height and area averaged (± SE) 3.2 ± 0.5 versus 3.6 7plusmn; 0.3 mm, and 2.3 ± 0.5 versus 2.6 ± 0.8mm2 for coronal-load and soak-load implants, respectively (P <0.05). The corresponding bone density and bone-implant contact registrations averaged 46.7% ± 5.8% versus 31.6% ± 4.4%, and 28% ± 5.6% versus 36.9% ± 3.4% (P >0.05). In contrast, resident bone remodeling was significantly influenced by the rhBMP-2 application protocol. Peri-implant bone density averaged 72.2% ± 2.1% for coronal-load versus 60.6% ± 4.7% for soak-load implants (P <0.05); the corresponding bone-implant contact averaged 70.7% ± 6.1% versus 47.2% ± 6.0% (P <0.05). Conclusions: Local application of rhBMP-2 and vacuum-drying using industrial process seems to be a viable technology to manufacture implants that support local bone formation and osseointegration. Coronalload implants obviate resident bone remodeling without compromisig local bone formation. J Periodontol 2010;81:1839-1849.

Original languageEnglish (US)
Pages (from-to)1839-1849
Number of pages11
JournalJournal of Periodontology
Volume81
Issue number12
DOIs
StatePublished - Dec 1 2010

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Vacuum
Dogs
Osteogenesis
Bone Remodeling
Newfoundland and Labrador
Osseointegration
Bone and Bones
Immersion
recombinant human bone morphogenetic protein-2
Bone Density
Air
Technology

Keywords

  • Bone density
  • Dental implants
  • Osseointegration
  • Osteogenesis
  • Recombinant
  • RhBMP-2 protein
  • Tissue engineering

ASJC Scopus subject areas

  • Periodontics

Cite this

Evaluation of implants coated with recombinant human bone morphogenetic protein-2 and vacuum-dried using the critical-size supraalveolar peri-implant defect model in dogs. / Decker, John F.; Lee, Jaebum; COrtella, Carlo Alberto; Polimeni, Giuseppe; Rohrer, Michael D.; Wozney, John M.; Hall, Jan; Susin, Cristiano; Wikesjö, Ulf M E.

In: Journal of Periodontology, Vol. 81, No. 12, 01.12.2010, p. 1839-1849.

Research output: Contribution to journalArticle

Decker, John F. ; Lee, Jaebum ; COrtella, Carlo Alberto ; Polimeni, Giuseppe ; Rohrer, Michael D. ; Wozney, John M. ; Hall, Jan ; Susin, Cristiano ; Wikesjö, Ulf M E. / Evaluation of implants coated with recombinant human bone morphogenetic protein-2 and vacuum-dried using the critical-size supraalveolar peri-implant defect model in dogs. In: Journal of Periodontology. 2010 ; Vol. 81, No. 12. pp. 1839-1849.
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title = "Evaluation of implants coated with recombinant human bone morphogenetic protein-2 and vacuum-dried using the critical-size supraalveolar peri-implant defect model in dogs",
abstract = "Background: Endosseous implants coated with recombinant human bone morphogenetic protein-2 (rhBMP-2) in a laboratory bench setting and air-dried induce relevant bone formation but also resident bone remodeling. Thus, the objective of this study is to evaluate the effect of implants fully or partially coated with rhBMP-2 and vacuum-dried using an industrial process on local bone formation and resident bone remodeling. Methods: Twelve male adult Hound Labrador mongrel dogs were used. Critical-size, supraalveolar, periimplant defects received titanium porous oxide surface implants coated in their most coronal aspect with rhBMP-2 (coronal-load, six animals), or by immersion of the entire implant in a rhBMP-2 solution (soakload, six animals) for a total of 30 μg rhBMP-2 per implant. All implants were vacuum-dried. The animals were sacrificed at 8 weeks for histometric evaluation. Results: Clinical healing was unremarkable. Bone formation was not significantly affected by the rhBMP- 2 application protocol. New bone height and area averaged (± SE) 3.2 ± 0.5 versus 3.6 7plusmn; 0.3 mm, and 2.3 ± 0.5 versus 2.6 ± 0.8mm2 for coronal-load and soak-load implants, respectively (P <0.05). The corresponding bone density and bone-implant contact registrations averaged 46.7{\%} ± 5.8{\%} versus 31.6{\%} ± 4.4{\%}, and 28{\%} ± 5.6{\%} versus 36.9{\%} ± 3.4{\%} (P >0.05). In contrast, resident bone remodeling was significantly influenced by the rhBMP-2 application protocol. Peri-implant bone density averaged 72.2{\%} ± 2.1{\%} for coronal-load versus 60.6{\%} ± 4.7{\%} for soak-load implants (P <0.05); the corresponding bone-implant contact averaged 70.7{\%} ± 6.1{\%} versus 47.2{\%} ± 6.0{\%} (P <0.05). Conclusions: Local application of rhBMP-2 and vacuum-drying using industrial process seems to be a viable technology to manufacture implants that support local bone formation and osseointegration. Coronalload implants obviate resident bone remodeling without compromisig local bone formation. J Periodontol 2010;81:1839-1849.",
keywords = "Bone density, Dental implants, Osseointegration, Osteogenesis, Recombinant, RhBMP-2 protein, Tissue engineering",
author = "Decker, {John F.} and Jaebum Lee and COrtella, {Carlo Alberto} and Giuseppe Polimeni and Rohrer, {Michael D.} and Wozney, {John M.} and Jan Hall and Cristiano Susin and Wikesj{\"o}, {Ulf M E}",
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T1 - Evaluation of implants coated with recombinant human bone morphogenetic protein-2 and vacuum-dried using the critical-size supraalveolar peri-implant defect model in dogs

AU - Decker, John F.

AU - Lee, Jaebum

AU - COrtella, Carlo Alberto

AU - Polimeni, Giuseppe

AU - Rohrer, Michael D.

AU - Wozney, John M.

AU - Hall, Jan

AU - Susin, Cristiano

AU - Wikesjö, Ulf M E

PY - 2010/12/1

Y1 - 2010/12/1

N2 - Background: Endosseous implants coated with recombinant human bone morphogenetic protein-2 (rhBMP-2) in a laboratory bench setting and air-dried induce relevant bone formation but also resident bone remodeling. Thus, the objective of this study is to evaluate the effect of implants fully or partially coated with rhBMP-2 and vacuum-dried using an industrial process on local bone formation and resident bone remodeling. Methods: Twelve male adult Hound Labrador mongrel dogs were used. Critical-size, supraalveolar, periimplant defects received titanium porous oxide surface implants coated in their most coronal aspect with rhBMP-2 (coronal-load, six animals), or by immersion of the entire implant in a rhBMP-2 solution (soakload, six animals) for a total of 30 μg rhBMP-2 per implant. All implants were vacuum-dried. The animals were sacrificed at 8 weeks for histometric evaluation. Results: Clinical healing was unremarkable. Bone formation was not significantly affected by the rhBMP- 2 application protocol. New bone height and area averaged (± SE) 3.2 ± 0.5 versus 3.6 7plusmn; 0.3 mm, and 2.3 ± 0.5 versus 2.6 ± 0.8mm2 for coronal-load and soak-load implants, respectively (P <0.05). The corresponding bone density and bone-implant contact registrations averaged 46.7% ± 5.8% versus 31.6% ± 4.4%, and 28% ± 5.6% versus 36.9% ± 3.4% (P >0.05). In contrast, resident bone remodeling was significantly influenced by the rhBMP-2 application protocol. Peri-implant bone density averaged 72.2% ± 2.1% for coronal-load versus 60.6% ± 4.7% for soak-load implants (P <0.05); the corresponding bone-implant contact averaged 70.7% ± 6.1% versus 47.2% ± 6.0% (P <0.05). Conclusions: Local application of rhBMP-2 and vacuum-drying using industrial process seems to be a viable technology to manufacture implants that support local bone formation and osseointegration. Coronalload implants obviate resident bone remodeling without compromisig local bone formation. J Periodontol 2010;81:1839-1849.

AB - Background: Endosseous implants coated with recombinant human bone morphogenetic protein-2 (rhBMP-2) in a laboratory bench setting and air-dried induce relevant bone formation but also resident bone remodeling. Thus, the objective of this study is to evaluate the effect of implants fully or partially coated with rhBMP-2 and vacuum-dried using an industrial process on local bone formation and resident bone remodeling. Methods: Twelve male adult Hound Labrador mongrel dogs were used. Critical-size, supraalveolar, periimplant defects received titanium porous oxide surface implants coated in their most coronal aspect with rhBMP-2 (coronal-load, six animals), or by immersion of the entire implant in a rhBMP-2 solution (soakload, six animals) for a total of 30 μg rhBMP-2 per implant. All implants were vacuum-dried. The animals were sacrificed at 8 weeks for histometric evaluation. Results: Clinical healing was unremarkable. Bone formation was not significantly affected by the rhBMP- 2 application protocol. New bone height and area averaged (± SE) 3.2 ± 0.5 versus 3.6 7plusmn; 0.3 mm, and 2.3 ± 0.5 versus 2.6 ± 0.8mm2 for coronal-load and soak-load implants, respectively (P <0.05). The corresponding bone density and bone-implant contact registrations averaged 46.7% ± 5.8% versus 31.6% ± 4.4%, and 28% ± 5.6% versus 36.9% ± 3.4% (P >0.05). In contrast, resident bone remodeling was significantly influenced by the rhBMP-2 application protocol. Peri-implant bone density averaged 72.2% ± 2.1% for coronal-load versus 60.6% ± 4.7% for soak-load implants (P <0.05); the corresponding bone-implant contact averaged 70.7% ± 6.1% versus 47.2% ± 6.0% (P <0.05). Conclusions: Local application of rhBMP-2 and vacuum-drying using industrial process seems to be a viable technology to manufacture implants that support local bone formation and osseointegration. Coronalload implants obviate resident bone remodeling without compromisig local bone formation. J Periodontol 2010;81:1839-1849.

KW - Bone density

KW - Dental implants

KW - Osseointegration

KW - Osteogenesis

KW - Recombinant

KW - RhBMP-2 protein

KW - Tissue engineering

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