Evaluation of micrometastases in sentinel lymph nodes of cutaneous melanoma: Higher diagnostic accuracy with Melan-A and MART-1 compared with S-100 protein and HMB-45

Vinod B. Shidham, Dan Yi Qi, Scott Acker, Bal Kampalath, Chung Che Chang, Varghese George, Richard Komorowski

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Accurate diagnosis of micrometastases in sentinel lymph nodes of cutaneous melanoma is critical for proper clinical management. S-100 protein and HMB-45 are the traditional immunomarkers widely used for this purpose. However, the interpretation of micrometastases by these markers is difficult with significant reduction in the diagnostic accuracy. S-100 protein demonstrates immunoreactivity for other nonmelanoma cells and obscures nuclear details, which are crucial for the interpretation of single cell metastases. We compared the new melanoma markers, Melan-A (clone A103) and MART-1 (clone M2-7C10), with S-100 protein and HMB-45, by examining 77 formalin-fixed paraffin-embedded sections of sentinel lymph nodes from 13 cases of primary cutaneous melanoma. CD68 (PG-M1) and hematoxylin-eosin-stained sections were also studied. Four pathologists interpreted the staining pattern after concealing the identity of each immunomarker. Az values (area under receiver operating characteristic curve) with receiver operating characteristic curve were higher with Melan-A (0.9742) and MART-1 (0.9779) compared with S-100 protein (0.8034) and HMB-45 (0.8651), demonstrating a higher diagnostic accuracy with Melan-A and MART-1 with superior detection of melanoma micrometastases. Melan-A and MART-1 showed sharp cytoplasmic immunoreactivity, almost exclusively restricted to the melanoma cells. Therefore, Melan-A and MART-1 are recommended for the evaluation of micrometastases in sentinel lymph nodes of cutaneous melanoma as a routine alternative to S-100 protein and HMB-45.

Original languageEnglish (US)
Pages (from-to)1039-1046
Number of pages8
JournalAmerican Journal of Surgical Pathology
Volume25
Issue number8
DOIs
StatePublished - Aug 8 2001
Externally publishedYes

Fingerprint

MART-1 Antigen
Neoplasm Micrometastasis
S100 Proteins
Melanoma
Skin
ROC Curve
A 103
Clone Cells
Hematoxylin
Eosine Yellowish-(YS)
Paraffin
Formaldehyde
Sentinel Lymph Node
Staining and Labeling
Neoplasm Metastasis

Keywords

  • HMB-45
  • Lymphadenectomy
  • MART- 1
  • Melan-A
  • Melanoma
  • Micrometastasis
  • PG-M1
  • S-100 protein
  • Sentinel lymph node

ASJC Scopus subject areas

  • Anatomy
  • Surgery
  • Pathology and Forensic Medicine

Cite this

Evaluation of micrometastases in sentinel lymph nodes of cutaneous melanoma : Higher diagnostic accuracy with Melan-A and MART-1 compared with S-100 protein and HMB-45. / Shidham, Vinod B.; Qi, Dan Yi; Acker, Scott; Kampalath, Bal; Chang, Chung Che; George, Varghese; Komorowski, Richard.

In: American Journal of Surgical Pathology, Vol. 25, No. 8, 08.08.2001, p. 1039-1046.

Research output: Contribution to journalArticle

Shidham, Vinod B. ; Qi, Dan Yi ; Acker, Scott ; Kampalath, Bal ; Chang, Chung Che ; George, Varghese ; Komorowski, Richard. / Evaluation of micrometastases in sentinel lymph nodes of cutaneous melanoma : Higher diagnostic accuracy with Melan-A and MART-1 compared with S-100 protein and HMB-45. In: American Journal of Surgical Pathology. 2001 ; Vol. 25, No. 8. pp. 1039-1046.
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abstract = "Accurate diagnosis of micrometastases in sentinel lymph nodes of cutaneous melanoma is critical for proper clinical management. S-100 protein and HMB-45 are the traditional immunomarkers widely used for this purpose. However, the interpretation of micrometastases by these markers is difficult with significant reduction in the diagnostic accuracy. S-100 protein demonstrates immunoreactivity for other nonmelanoma cells and obscures nuclear details, which are crucial for the interpretation of single cell metastases. We compared the new melanoma markers, Melan-A (clone A103) and MART-1 (clone M2-7C10), with S-100 protein and HMB-45, by examining 77 formalin-fixed paraffin-embedded sections of sentinel lymph nodes from 13 cases of primary cutaneous melanoma. CD68 (PG-M1) and hematoxylin-eosin-stained sections were also studied. Four pathologists interpreted the staining pattern after concealing the identity of each immunomarker. Az values (area under receiver operating characteristic curve) with receiver operating characteristic curve were higher with Melan-A (0.9742) and MART-1 (0.9779) compared with S-100 protein (0.8034) and HMB-45 (0.8651), demonstrating a higher diagnostic accuracy with Melan-A and MART-1 with superior detection of melanoma micrometastases. Melan-A and MART-1 showed sharp cytoplasmic immunoreactivity, almost exclusively restricted to the melanoma cells. Therefore, Melan-A and MART-1 are recommended for the evaluation of micrometastases in sentinel lymph nodes of cutaneous melanoma as a routine alternative to S-100 protein and HMB-45.",
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AU - Qi, Dan Yi

AU - Acker, Scott

AU - Kampalath, Bal

AU - Chang, Chung Che

AU - George, Varghese

AU - Komorowski, Richard

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