Evaluations of metabolic activities as biocompatibility tools

A study of individual ions' effects on fibroblasts

Regina L W Messer, L. C. Lucas

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Objectives: Nickel-based dental alloys have been in use since 1930. However, there are concerns regarding the release of metal ions from these alloys to surrounding tissues. Cell culture evaluations can be used to address these concerns and to develop a biocompatibility model by providing a more basic understanding of the metabolic response to individual ions released from dental alloys. This study evaluates the metabolic as well as the morphological response of cultured human gingival fibroblasts to salt solutions of ions which may be released from these alloys; beryllium (Be2+), chromium (Cr6+ and Cr3+), nickel (Ni2+), molybdenum (Mo6+). Methods: These evaluations include viability, lysosomal activity, oxygen consumption, membrane integrity, DNA synthesis, RNA synthesis, protein synthesis, intracellular ATP levels, and glucose-6-phosphate dehydrogenase activity. The results of all cell culture evaluations are reported as the concentration (ppm) required to cause a significant change from the controls, as determined by Duncan's multiple comparison test at 0.05 significance level. Results: While Ni2+ ion solutions altered metabolic functions at 3-30 ppm and Cr3+ and Mo6+ both at 10 and 100 ppm, Cr6+ and Be2+ were the most toxic causing cellular alterations at 0.04-12 ppm. Significance: These studies indicated that monitoring metabolic activities may be better than the normally used morphology and viability assays for evaluating biocompatibility.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalDental Materials
Volume15
Issue number1
DOIs
StatePublished - Jan 1 1999
Externally publishedYes

Fingerprint

Fibroblasts
Dental alloys
Biocompatibility
Dental Alloys
Ions
Nickel
Cell culture
Beryllium
Molybdenum
Cell Culture Techniques
Glucosephosphate Dehydrogenase
Poisons
Adenosinetriphosphate
Chromium
RNA
Metal ions
Glucose
Assays
Phosphates
DNA

Keywords

  • Biocompatibility
  • Chromium
  • Dental alloys
  • Metabolism
  • Metal ions
  • Nickel
  • Salts

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Evaluations of metabolic activities as biocompatibility tools : A study of individual ions' effects on fibroblasts. / Messer, Regina L W; Lucas, L. C.

In: Dental Materials, Vol. 15, No. 1, 01.01.1999, p. 1-6.

Research output: Contribution to journalArticle

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