EVI5 is a novel centrosomal protein that binds to α- and γ-tubulin

Silviu L. Faitar, Jeremy T S Dabbeekeh, Tamara A. Ranalli, John Kenneth Cowell

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The human EVI5 protein carries a TBC domain indicative of Rab GTPase activating protein (GAP) activity, and an extensive coiled-coil motif in the C-terminal region. EVI5 is ubiquitously expressed in adult, fetal, and cancer tissues and exists as two mRNA species resulting from differential use of polyadenylation signals. Western blot analysis suggests that different molecular weight protein species are probably generated by posttranslational modification. FPLC analysis demonstrates that EVI5 protein can form dimers and confocal microscopy indicates that EVI5, in addition to a diffuse localization in the nucleus, also preferentially localizes to the pericentriolar material in interphase cells. Immunoprecipitation and GST pull-down experiments demonstrate that EVI5 exists in complexes with both α- and γ-tubulin. Both interactions are localized to the N-terminal part of the EVI5 protein. Thus, EVI5 is a novel centrosomal protein with a complex expression pattern and subcellular localization, possibly involved in centrosome stability and dynamics.

Original languageEnglish (US)
Pages (from-to)594-605
Number of pages12
JournalGenomics
Volume86
Issue number5
DOIs
StatePublished - Nov 1 2005
Externally publishedYes

Fingerprint

Tubulin
Proteins
rab GTP-Binding Proteins
GTPase-Activating Proteins
Centrosome
Polyadenylation
Interphase
Post Translational Protein Processing
Immunoprecipitation
Confocal Microscopy
Fetus
Molecular Weight
Western Blotting
Messenger RNA
Neoplasms

Keywords

  • Centrosome
  • EVI gene
  • EVI5 expression pattern
  • Tubulin binding protein

ASJC Scopus subject areas

  • Genetics

Cite this

EVI5 is a novel centrosomal protein that binds to α- and γ-tubulin. / Faitar, Silviu L.; Dabbeekeh, Jeremy T S; Ranalli, Tamara A.; Cowell, John Kenneth.

In: Genomics, Vol. 86, No. 5, 01.11.2005, p. 594-605.

Research output: Contribution to journalArticle

Faitar, Silviu L. ; Dabbeekeh, Jeremy T S ; Ranalli, Tamara A. ; Cowell, John Kenneth. / EVI5 is a novel centrosomal protein that binds to α- and γ-tubulin. In: Genomics. 2005 ; Vol. 86, No. 5. pp. 594-605.
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