Evidence for μ opiate receptors on inhibitory terminals in area CA1 of rat hippocampus

Nevin A Lambert, Neil L. Harrison, Timothy J. Teyler

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The mechanism of disinhibition produced by opioid peptides was studied using intracellular recording in area CA1 of rat hippocampal slices. The μ-selective opioid peptide [d-Ala2,N-Me-Phe4,Gly-ol5]-enkephalin (DAGO) reversibly depressed directly-activated, monosynaptic inhibitory postsynaptic potentials (IPSPs) evoked in the presence of the excitatory amino acid receptor antagonists 6,7-dinitroquinoxaline-2,3-dione (DNQX) and d,l-2-amino-5-phosphonovalerate (APV) in a naloxone-sensitive manner. Depression of monosynaptic inhibitory postsynaptic potentials (IPSPs) by DAGO was not prevented by 1-2 mM Ba2+. DAGO reversibly depressed monosynaptic IPSPs when applied locally close to the recording site, but was ineffective when applied close to the stimulating site in stratum radiatum. These results suggest that DAGO disinhibits pyramidal neurons in area CA1 of the rat hippocampus by activating μ opiate receptors located on the terminals of inhibitory neurons, and by a Ba2+-insensitive mechanism.

Original languageEnglish (US)
Pages (from-to)101-104
Number of pages4
JournalNeuroscience Letters
Volume124
Issue number1
DOIs
StatePublished - Mar 11 1991
Externally publishedYes

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Ala(2)-MePhe(4)-Gly(5)-enkephalin
Opioid Receptors
Inhibitory Postsynaptic Potentials
Hippocampus
Opioid Peptides
2-Amino-5-phosphonovalerate
Excitatory Amino Acid Antagonists
Enkephalins
Pyramidal Cells
Glutamate Receptors
Naloxone
Neurons

Keywords

  • DAGO
  • Disinhibition
  • Interneuron
  • Opioid peptide

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Evidence for μ opiate receptors on inhibitory terminals in area CA1 of rat hippocampus. / Lambert, Nevin A; Harrison, Neil L.; Teyler, Timothy J.

In: Neuroscience Letters, Vol. 124, No. 1, 11.03.1991, p. 101-104.

Research output: Contribution to journalArticle

Lambert, Nevin A ; Harrison, Neil L. ; Teyler, Timothy J. / Evidence for μ opiate receptors on inhibitory terminals in area CA1 of rat hippocampus. In: Neuroscience Letters. 1991 ; Vol. 124, No. 1. pp. 101-104.
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