Evidence for a spatial and temporal regulation of prostaglandin- endoperoxide synthase 2 expression in human amnion in term and preterm parturition

Deug Chan Lee, Roberto Romero, Jung Sun Kim, Wonsuk Yoo, Joon Ho Lee, Pooja Mittal, Juan Pedro Kusanovic, Sonia S. Hassan, Bo Hyun Yoon, Chong Jai Kim

Research output: Contribution to journalArticle

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Abstract

Context: Prostaglandin-endoperoxide synthase 2 (PTGS2) is a key enzyme involved in parturition. PTGS2 mRNA was found to be differentially expressed between placental amnion (amnion overlying the placental disc) and reflected amnion (amnion of the extraplacental chorioamniotic membranes) in term placentas. Objective: The aim was to evaluate the spatial and temporal regulation of PTGS2 expression in the amnion and the chorion-decidua. Design: PTGS2 expression was analyzed in the amnion and chorion-decidua obtained from 32 women: term not in labor (n = 12), term in labor (n = 12), and preterm labor (n = 8), by immunoblotting and densitometry. Prostaglandin E 2 (PGE 2 ) in the amnion and chorion-decidua was measured by a specific immunoassay. Results: Compared to preterm labor cases, PTGS2 expression increased at term before the onset of labor far more prominently in placental amnion (4.5-fold; P = 0.002) than in reflected amnion (1.4-fold; P = 0.007). There was a significant increase in PTGS2 expression in reflected amnion (2.9-fold; P < 0.01) but not in placental amnion with labor at term. PTGS2 expression was higher in reflected amnion than in chorion-decidua in labor at term (2.9-fold; P<0.01). PTGS2 was barely detected in amnion and chorion-decidua with preterm labor. Expression of PGE 2 showed a good correlation with PTGS2 expression (r = 0.722; P < 0.001). Conclusion: PTGS2 expression in the amnion shows a distinct spatial and temporal regulation. Spontaneous labor at term and pathological preterm labor clearly differ in amniotic PTGS2 and PGE 2 abundance. Our observations underscore the biological significance of the amnion and amniotic fluid in human parturition.

Original languageEnglish (US)
Pages (from-to)E86-E91
JournalJournal of Clinical Endocrinology and Metabolism
Volume95
Issue number9
DOIs
StatePublished - Sep 2010

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Amnion
Prostaglandin-Endoperoxide Synthases
Parturition
Personnel
Chorion
Decidua
Premature Obstetric Labor
Prostaglandins E
Labor Onset
Densitometry
Amniotic Fluid
Immunoassay
Immunoblotting
Placenta
Membranes

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Evidence for a spatial and temporal regulation of prostaglandin- endoperoxide synthase 2 expression in human amnion in term and preterm parturition. / Lee, Deug Chan; Romero, Roberto; Kim, Jung Sun; Yoo, Wonsuk; Lee, Joon Ho; Mittal, Pooja; Kusanovic, Juan Pedro; Hassan, Sonia S.; Yoon, Bo Hyun; Kim, Chong Jai.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 95, No. 9, 09.2010, p. E86-E91.

Research output: Contribution to journalArticle

Lee, Deug Chan ; Romero, Roberto ; Kim, Jung Sun ; Yoo, Wonsuk ; Lee, Joon Ho ; Mittal, Pooja ; Kusanovic, Juan Pedro ; Hassan, Sonia S. ; Yoon, Bo Hyun ; Kim, Chong Jai. / Evidence for a spatial and temporal regulation of prostaglandin- endoperoxide synthase 2 expression in human amnion in term and preterm parturition. In: Journal of Clinical Endocrinology and Metabolism. 2010 ; Vol. 95, No. 9. pp. E86-E91.
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abstract = "Context: Prostaglandin-endoperoxide synthase 2 (PTGS2) is a key enzyme involved in parturition. PTGS2 mRNA was found to be differentially expressed between placental amnion (amnion overlying the placental disc) and reflected amnion (amnion of the extraplacental chorioamniotic membranes) in term placentas. Objective: The aim was to evaluate the spatial and temporal regulation of PTGS2 expression in the amnion and the chorion-decidua. Design: PTGS2 expression was analyzed in the amnion and chorion-decidua obtained from 32 women: term not in labor (n = 12), term in labor (n = 12), and preterm labor (n = 8), by immunoblotting and densitometry. Prostaglandin E 2 (PGE 2 ) in the amnion and chorion-decidua was measured by a specific immunoassay. Results: Compared to preterm labor cases, PTGS2 expression increased at term before the onset of labor far more prominently in placental amnion (4.5-fold; P = 0.002) than in reflected amnion (1.4-fold; P = 0.007). There was a significant increase in PTGS2 expression in reflected amnion (2.9-fold; P < 0.01) but not in placental amnion with labor at term. PTGS2 expression was higher in reflected amnion than in chorion-decidua in labor at term (2.9-fold; P<0.01). PTGS2 was barely detected in amnion and chorion-decidua with preterm labor. Expression of PGE 2 showed a good correlation with PTGS2 expression (r = 0.722; P < 0.001). Conclusion: PTGS2 expression in the amnion shows a distinct spatial and temporal regulation. Spontaneous labor at term and pathological preterm labor clearly differ in amniotic PTGS2 and PGE 2 abundance. Our observations underscore the biological significance of the amnion and amniotic fluid in human parturition.",
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T1 - Evidence for a spatial and temporal regulation of prostaglandin- endoperoxide synthase 2 expression in human amnion in term and preterm parturition

AU - Lee, Deug Chan

AU - Romero, Roberto

AU - Kim, Jung Sun

AU - Yoo, Wonsuk

AU - Lee, Joon Ho

AU - Mittal, Pooja

AU - Kusanovic, Juan Pedro

AU - Hassan, Sonia S.

AU - Yoon, Bo Hyun

AU - Kim, Chong Jai

PY - 2010/9

Y1 - 2010/9

N2 - Context: Prostaglandin-endoperoxide synthase 2 (PTGS2) is a key enzyme involved in parturition. PTGS2 mRNA was found to be differentially expressed between placental amnion (amnion overlying the placental disc) and reflected amnion (amnion of the extraplacental chorioamniotic membranes) in term placentas. Objective: The aim was to evaluate the spatial and temporal regulation of PTGS2 expression in the amnion and the chorion-decidua. Design: PTGS2 expression was analyzed in the amnion and chorion-decidua obtained from 32 women: term not in labor (n = 12), term in labor (n = 12), and preterm labor (n = 8), by immunoblotting and densitometry. Prostaglandin E 2 (PGE 2 ) in the amnion and chorion-decidua was measured by a specific immunoassay. Results: Compared to preterm labor cases, PTGS2 expression increased at term before the onset of labor far more prominently in placental amnion (4.5-fold; P = 0.002) than in reflected amnion (1.4-fold; P = 0.007). There was a significant increase in PTGS2 expression in reflected amnion (2.9-fold; P < 0.01) but not in placental amnion with labor at term. PTGS2 expression was higher in reflected amnion than in chorion-decidua in labor at term (2.9-fold; P<0.01). PTGS2 was barely detected in amnion and chorion-decidua with preterm labor. Expression of PGE 2 showed a good correlation with PTGS2 expression (r = 0.722; P < 0.001). Conclusion: PTGS2 expression in the amnion shows a distinct spatial and temporal regulation. Spontaneous labor at term and pathological preterm labor clearly differ in amniotic PTGS2 and PGE 2 abundance. Our observations underscore the biological significance of the amnion and amniotic fluid in human parturition.

AB - Context: Prostaglandin-endoperoxide synthase 2 (PTGS2) is a key enzyme involved in parturition. PTGS2 mRNA was found to be differentially expressed between placental amnion (amnion overlying the placental disc) and reflected amnion (amnion of the extraplacental chorioamniotic membranes) in term placentas. Objective: The aim was to evaluate the spatial and temporal regulation of PTGS2 expression in the amnion and the chorion-decidua. Design: PTGS2 expression was analyzed in the amnion and chorion-decidua obtained from 32 women: term not in labor (n = 12), term in labor (n = 12), and preterm labor (n = 8), by immunoblotting and densitometry. Prostaglandin E 2 (PGE 2 ) in the amnion and chorion-decidua was measured by a specific immunoassay. Results: Compared to preterm labor cases, PTGS2 expression increased at term before the onset of labor far more prominently in placental amnion (4.5-fold; P = 0.002) than in reflected amnion (1.4-fold; P = 0.007). There was a significant increase in PTGS2 expression in reflected amnion (2.9-fold; P < 0.01) but not in placental amnion with labor at term. PTGS2 expression was higher in reflected amnion than in chorion-decidua in labor at term (2.9-fold; P<0.01). PTGS2 was barely detected in amnion and chorion-decidua with preterm labor. Expression of PGE 2 showed a good correlation with PTGS2 expression (r = 0.722; P < 0.001). Conclusion: PTGS2 expression in the amnion shows a distinct spatial and temporal regulation. Spontaneous labor at term and pathological preterm labor clearly differ in amniotic PTGS2 and PGE 2 abundance. Our observations underscore the biological significance of the amnion and amniotic fluid in human parturition.

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