Evidence for pseudomonas antigen in immune complexes in pseudomonas osteomyelitis

Daniel Wallace Rahn, Allen C. Steere, Robert L. Grodzicki, Joseph H. Levine, John A. Hardin

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In infections associated with immune complex disease, microbial antigens have rarely been found in the complexes. Using an enzyme‐linked immunoassay, we studied the immune complexes of a patient who had hematogenous Pseudomonas aeruginosa osteomyelitis associated with palpable purpura, arthritis, and microscopic hematuria. After separation of the complexes into high and low molecular weight fractions, a 6hyphen;fold selective concentration of P aeruginosa antibody was found in the low molecular weight fraction compared with the concentration in the serum. Following disruption of immunoglobulin, the high molecular weight fraction competed with solid‐phase P aeruginosa antigen for P aeruginosa antibody from another source. After successful treatment of the infection, the patient's symptoms resolved, and the complexes disappeared. These findings strongly suggest that immune complexes in this patient contained P aeruginosa antigen and antibody that may have been pathogenetic in his disease.

Original languageEnglish (US)
Pages (from-to)1403-1408
Number of pages6
JournalArthritis & Rheumatism
Volume25
Issue number12
DOIs
StatePublished - Jan 1 1982

Fingerprint

Osteomyelitis
Pseudomonas
Antigen-Antibody Complex
Molecular Weight
Antigens
Antibodies
Immune Complex Diseases
Purpura
Hematuria
Infection
Immunoassay
Pseudomonas aeruginosa
Arthritis
Immunoglobulins
Serum
Therapeutics

ASJC Scopus subject areas

  • Immunology and Allergy
  • Rheumatology
  • Immunology
  • Pharmacology (medical)

Cite this

Evidence for pseudomonas antigen in immune complexes in pseudomonas osteomyelitis. / Rahn, Daniel Wallace; Steere, Allen C.; Grodzicki, Robert L.; Levine, Joseph H.; Hardin, John A.

In: Arthritis & Rheumatism, Vol. 25, No. 12, 01.01.1982, p. 1403-1408.

Research output: Contribution to journalArticle

Rahn, Daniel Wallace ; Steere, Allen C. ; Grodzicki, Robert L. ; Levine, Joseph H. ; Hardin, John A. / Evidence for pseudomonas antigen in immune complexes in pseudomonas osteomyelitis. In: Arthritis & Rheumatism. 1982 ; Vol. 25, No. 12. pp. 1403-1408.
@article{4ca0178d4d3148759e91f6136728d72e,
title = "Evidence for pseudomonas antigen in immune complexes in pseudomonas osteomyelitis",
abstract = "In infections associated with immune complex disease, microbial antigens have rarely been found in the complexes. Using an enzyme‐linked immunoassay, we studied the immune complexes of a patient who had hematogenous Pseudomonas aeruginosa osteomyelitis associated with palpable purpura, arthritis, and microscopic hematuria. After separation of the complexes into high and low molecular weight fractions, a 6hyphen;fold selective concentration of P aeruginosa antibody was found in the low molecular weight fraction compared with the concentration in the serum. Following disruption of immunoglobulin, the high molecular weight fraction competed with solid‐phase P aeruginosa antigen for P aeruginosa antibody from another source. After successful treatment of the infection, the patient's symptoms resolved, and the complexes disappeared. These findings strongly suggest that immune complexes in this patient contained P aeruginosa antigen and antibody that may have been pathogenetic in his disease.",
author = "Rahn, {Daniel Wallace} and Steere, {Allen C.} and Grodzicki, {Robert L.} and Levine, {Joseph H.} and Hardin, {John A.}",
year = "1982",
month = "1",
day = "1",
doi = "10.1002/art.1780251203",
language = "English (US)",
volume = "25",
pages = "1403--1408",
journal = "Arthritis and Rheumatology",
issn = "2326-5191",
publisher = "John Wiley and Sons Ltd",
number = "12",

}

TY - JOUR

T1 - Evidence for pseudomonas antigen in immune complexes in pseudomonas osteomyelitis

AU - Rahn, Daniel Wallace

AU - Steere, Allen C.

AU - Grodzicki, Robert L.

AU - Levine, Joseph H.

AU - Hardin, John A.

PY - 1982/1/1

Y1 - 1982/1/1

N2 - In infections associated with immune complex disease, microbial antigens have rarely been found in the complexes. Using an enzyme‐linked immunoassay, we studied the immune complexes of a patient who had hematogenous Pseudomonas aeruginosa osteomyelitis associated with palpable purpura, arthritis, and microscopic hematuria. After separation of the complexes into high and low molecular weight fractions, a 6hyphen;fold selective concentration of P aeruginosa antibody was found in the low molecular weight fraction compared with the concentration in the serum. Following disruption of immunoglobulin, the high molecular weight fraction competed with solid‐phase P aeruginosa antigen for P aeruginosa antibody from another source. After successful treatment of the infection, the patient's symptoms resolved, and the complexes disappeared. These findings strongly suggest that immune complexes in this patient contained P aeruginosa antigen and antibody that may have been pathogenetic in his disease.

AB - In infections associated with immune complex disease, microbial antigens have rarely been found in the complexes. Using an enzyme‐linked immunoassay, we studied the immune complexes of a patient who had hematogenous Pseudomonas aeruginosa osteomyelitis associated with palpable purpura, arthritis, and microscopic hematuria. After separation of the complexes into high and low molecular weight fractions, a 6hyphen;fold selective concentration of P aeruginosa antibody was found in the low molecular weight fraction compared with the concentration in the serum. Following disruption of immunoglobulin, the high molecular weight fraction competed with solid‐phase P aeruginosa antigen for P aeruginosa antibody from another source. After successful treatment of the infection, the patient's symptoms resolved, and the complexes disappeared. These findings strongly suggest that immune complexes in this patient contained P aeruginosa antigen and antibody that may have been pathogenetic in his disease.

UR - http://www.scopus.com/inward/record.url?scp=0020365010&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020365010&partnerID=8YFLogxK

U2 - 10.1002/art.1780251203

DO - 10.1002/art.1780251203

M3 - Article

VL - 25

SP - 1403

EP - 1408

JO - Arthritis and Rheumatology

JF - Arthritis and Rheumatology

SN - 2326-5191

IS - 12

ER -