Evidence for separate modes of action in thermal radiosensitization and direct thermal cell death

Nahid F Mivechi, Kurt G. Hofer

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

It is not known whether heat potentiation of radiation damage and direct heat death are mediated by the same or by different heat lesions within the cell. In this study, three types of experiments were performed on BP‐8 murine sarcoma cells to provide evidence against a common mode of action: (1)Evaluation of the kinetics of cell death from heat, radiation, and combined heat‐radiation treatments: direct heat death is rapid and essentially complete within 24‐48 hours after heat exposure, whereas radiation death develops only after a delay period of several days. Radiosensitization by heat affects only the delayed component of cell death, that is, the radiation component of death. (2)Thermal radiosensitization and direct heat death as a function of heating time: thermal radiosensitization requires only short exposures to heat; prolonged heating does not further enhance this effect. In contrast, direct heat death increases with increasing duration of heat exposure. (3)Independent modification of radiosensitization and thermal death: addition of 5% glycerol to the incubation medium protects cells against direct heat death, but not against thermal radiosensitization. In combination, these findings suggest that heat potentiation of radiation lethality and direct heat death are two distinct phenomena mediated by different cellular mechanisms.

Original languageEnglish (US)
Pages (from-to)38-43
Number of pages6
JournalCancer
Volume51
Issue number1
DOIs
StatePublished - Jan 1 1983

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Cell Death
Hot Temperature
Radiation
Heating
Sarcoma
Glycerol

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Evidence for separate modes of action in thermal radiosensitization and direct thermal cell death. / Mivechi, Nahid F; Hofer, Kurt G.

In: Cancer, Vol. 51, No. 1, 01.01.1983, p. 38-43.

Research output: Contribution to journalArticle

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