Evidence for the transport of neutral as well as cationic amino acids by ATA3, a novel and liver-specific subtype of amino acid transport system A

Takahiro Hatanaka, Wei Huang, Ruan Ling, Puttur D Prasad, Mitsuru Sugawara, Frederick H. Leibach, Vadivel Ganapathy

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

We report here on the cloning and functional characterization of the third subtype of amino acid transport system A, designated ATA3 (amino acid transporter A3), from a human liver cell line. This transporter consists of 547 amino acids and is structurally related to the members of the glutamine transporter family. The human ATA3 (hATA3) exhibits 88% identity in amino acid sequence with rat ATA3. The gene coding for hATA3 contains 16 exons and is located on human chromosome 12q13. It is expressed almost exclusively in the liver. hATA3 mediates the transport of neutral amino acids including α-(methylamino)isobutyric acid (MeAIB), the model substrate for system A, in a Na+-coupled manner and the transport of cationic amino acids in a Na+-independent manner. The affinity of hATA3 for cationic amino acids is higher than for neutral amino acids. The transport function of hATA3 is thus similar to that of system y+L. The ability of hATA3 to transport cationic amino acids with high affinity is unique among the members of the glutamine transporter family. hATA1 and hATA2, the other two known members of the system A subfamily, show little affinity toward cationic amino acids. hATA3 also differs from hATA1 and hATA2 in exhibiting low affinity for MeAIB. Since liver does not express any of the previously known high-affinity cationic amino acid transporters, ATA3 is likely to provide the major route for the uptake of arginine in this tissue.

Original languageEnglish (US)
Pages (from-to)10-17
Number of pages8
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1510
Issue number1-2
DOIs
StatePublished - Feb 9 2001

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Amino Acid Transport System A
Amino Acid Transport Systems
Liver
Amino Acids
Neutral Amino Acids
Glutamine
Basic Amino Acid Transport Systems
Cloning
Chromosomes
Arginine
Rats
Exons
Human Chromosomes
Genes
Cells
Tissue
Organism Cloning
Amino Acid Sequence
Substrates

Keywords

  • Amino acid transporter A3
  • Cationic amino acid
  • Glutamine transporter family
  • Human
  • Liver
  • Neutral amino acid
  • System A

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this

Evidence for the transport of neutral as well as cationic amino acids by ATA3, a novel and liver-specific subtype of amino acid transport system A. / Hatanaka, Takahiro; Huang, Wei; Ling, Ruan; Prasad, Puttur D; Sugawara, Mitsuru; Leibach, Frederick H.; Ganapathy, Vadivel.

In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1510, No. 1-2, 09.02.2001, p. 10-17.

Research output: Contribution to journalArticle

Hatanaka, Takahiro ; Huang, Wei ; Ling, Ruan ; Prasad, Puttur D ; Sugawara, Mitsuru ; Leibach, Frederick H. ; Ganapathy, Vadivel. / Evidence for the transport of neutral as well as cationic amino acids by ATA3, a novel and liver-specific subtype of amino acid transport system A. In: Biochimica et Biophysica Acta - Biomembranes. 2001 ; Vol. 1510, No. 1-2. pp. 10-17.
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AU - Hatanaka, Takahiro

AU - Huang, Wei

AU - Ling, Ruan

AU - Prasad, Puttur D

AU - Sugawara, Mitsuru

AU - Leibach, Frederick H.

AU - Ganapathy, Vadivel

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N2 - We report here on the cloning and functional characterization of the third subtype of amino acid transport system A, designated ATA3 (amino acid transporter A3), from a human liver cell line. This transporter consists of 547 amino acids and is structurally related to the members of the glutamine transporter family. The human ATA3 (hATA3) exhibits 88% identity in amino acid sequence with rat ATA3. The gene coding for hATA3 contains 16 exons and is located on human chromosome 12q13. It is expressed almost exclusively in the liver. hATA3 mediates the transport of neutral amino acids including α-(methylamino)isobutyric acid (MeAIB), the model substrate for system A, in a Na+-coupled manner and the transport of cationic amino acids in a Na+-independent manner. The affinity of hATA3 for cationic amino acids is higher than for neutral amino acids. The transport function of hATA3 is thus similar to that of system y+L. The ability of hATA3 to transport cationic amino acids with high affinity is unique among the members of the glutamine transporter family. hATA1 and hATA2, the other two known members of the system A subfamily, show little affinity toward cationic amino acids. hATA3 also differs from hATA1 and hATA2 in exhibiting low affinity for MeAIB. Since liver does not express any of the previously known high-affinity cationic amino acid transporters, ATA3 is likely to provide the major route for the uptake of arginine in this tissue.

AB - We report here on the cloning and functional characterization of the third subtype of amino acid transport system A, designated ATA3 (amino acid transporter A3), from a human liver cell line. This transporter consists of 547 amino acids and is structurally related to the members of the glutamine transporter family. The human ATA3 (hATA3) exhibits 88% identity in amino acid sequence with rat ATA3. The gene coding for hATA3 contains 16 exons and is located on human chromosome 12q13. It is expressed almost exclusively in the liver. hATA3 mediates the transport of neutral amino acids including α-(methylamino)isobutyric acid (MeAIB), the model substrate for system A, in a Na+-coupled manner and the transport of cationic amino acids in a Na+-independent manner. The affinity of hATA3 for cationic amino acids is higher than for neutral amino acids. The transport function of hATA3 is thus similar to that of system y+L. The ability of hATA3 to transport cationic amino acids with high affinity is unique among the members of the glutamine transporter family. hATA1 and hATA2, the other two known members of the system A subfamily, show little affinity toward cationic amino acids. hATA3 also differs from hATA1 and hATA2 in exhibiting low affinity for MeAIB. Since liver does not express any of the previously known high-affinity cationic amino acid transporters, ATA3 is likely to provide the major route for the uptake of arginine in this tissue.

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