Existence of two γ subunits of the G proteins in brain

J. D. Robishaw, V. K. Kalman, C. R. Moomaw, Clive A. Slaughter

Research output: Contribution to journalArticle

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Abstract

Although amino acid sequences have been determined for the α and β subunits of G(s), G(i), and G(o), sequences have not been reported for the γ subunits of these G proteins. In the present paper, we determined the sequences of peptides prepared by partial proteolysis of two different forms of the γ subunit of G(s), G(i), and G(o) from bovine brain. Using oligonucleotide probes based on the sequences of two of these peptides, a cDNA clone was isolated from a bovine adrenal cDNA library. This clone contained a 0.9-kilobase cDNA insert that included an open reading frame of 213 bases encoding a 71-amino acid polypeptide with an estimated M(r) of 7850. The amino acid sequence predicted for the adrenal cDNA clone was identical to that determined for one form of the γ subunit from brain. In addition, an antibody to a peptide based on the predicted amino acid sequence of this cDNA clone reacted specifically with one of the brain γ subunits, indicating the adrenal cDNA clone encodes a γ subunit present in both adrenal gland and brain. Also, evidence is presented, demonstrating the existence of a second, structurally distinct, form of the γ subunit of G(s), G(i), and G(o) in brain.

Original languageEnglish (US)
Pages (from-to)15758-15761
Number of pages4
JournalJournal of Biological Chemistry
Volume264
Issue number27
StatePublished - Jan 1 1989
Externally publishedYes

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GTP-Binding Proteins
Brain
Complementary DNA
Clone Cells
Amino Acids
Amino Acid Sequence
Peptides
Proteolysis
Oligonucleotide Probes
Adrenal Glands
Gene Library
Open Reading Frames
Antibodies

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Robishaw, J. D., Kalman, V. K., Moomaw, C. R., & Slaughter, C. A. (1989). Existence of two γ subunits of the G proteins in brain. Journal of Biological Chemistry, 264(27), 15758-15761.

Existence of two γ subunits of the G proteins in brain. / Robishaw, J. D.; Kalman, V. K.; Moomaw, C. R.; Slaughter, Clive A.

In: Journal of Biological Chemistry, Vol. 264, No. 27, 01.01.1989, p. 15758-15761.

Research output: Contribution to journalArticle

Robishaw, JD, Kalman, VK, Moomaw, CR & Slaughter, CA 1989, 'Existence of two γ subunits of the G proteins in brain', Journal of Biological Chemistry, vol. 264, no. 27, pp. 15758-15761.
Robishaw JD, Kalman VK, Moomaw CR, Slaughter CA. Existence of two γ subunits of the G proteins in brain. Journal of Biological Chemistry. 1989 Jan 1;264(27):15758-15761.
Robishaw, J. D. ; Kalman, V. K. ; Moomaw, C. R. ; Slaughter, Clive A. / Existence of two γ subunits of the G proteins in brain. In: Journal of Biological Chemistry. 1989 ; Vol. 264, No. 27. pp. 15758-15761.
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