Existence of two γ subunits of the G proteins in brain

J. D. Robishaw, V. K. Kalman, C. R. Moomaw, Clive A. Slaughter

Research output: Contribution to journalArticle

59 Scopus citations

Abstract

Although amino acid sequences have been determined for the α and β subunits of G(s), G(i), and G(o), sequences have not been reported for the γ subunits of these G proteins. In the present paper, we determined the sequences of peptides prepared by partial proteolysis of two different forms of the γ subunit of G(s), G(i), and G(o) from bovine brain. Using oligonucleotide probes based on the sequences of two of these peptides, a cDNA clone was isolated from a bovine adrenal cDNA library. This clone contained a 0.9-kilobase cDNA insert that included an open reading frame of 213 bases encoding a 71-amino acid polypeptide with an estimated M(r) of 7850. The amino acid sequence predicted for the adrenal cDNA clone was identical to that determined for one form of the γ subunit from brain. In addition, an antibody to a peptide based on the predicted amino acid sequence of this cDNA clone reacted specifically with one of the brain γ subunits, indicating the adrenal cDNA clone encodes a γ subunit present in both adrenal gland and brain. Also, evidence is presented, demonstrating the existence of a second, structurally distinct, form of the γ subunit of G(s), G(i), and G(o) in brain.

Original languageEnglish (US)
Pages (from-to)15758-15761
Number of pages4
JournalJournal of Biological Chemistry
Volume264
Issue number27
StatePublished - Jan 1 1989
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Robishaw, J. D., Kalman, V. K., Moomaw, C. R., & Slaughter, C. A. (1989). Existence of two γ subunits of the G proteins in brain. Journal of Biological Chemistry, 264(27), 15758-15761.