TY - JOUR
T1 - Exogenous expression of caspase-14 induces tumor suppression in human salivary cancer cells by inhibiting tumor vascularization
AU - Wu, Mengjie
AU - Kodani, Isamu
AU - Dickinson, Douglas
AU - Huff, Frank
AU - Ogbureke, Kalu U.E.
AU - Qin, Haiyan
AU - Arun, Senthil
AU - Dulebohn, Rachel
AU - Al-Shabrawey, Mohamed
AU - Tawhk, Amany
AU - Prater, Susan
AU - Lewis, Jill
AU - Wataha, John
AU - Messer, Regina L W
AU - Hsu, Stephen
PY - 2009/10
Y1 - 2009/10
N2 - Background: Current therapeutic approaches to salivary gland cancer are often associated with severe disfigurement and loss of glandular function, which are traumatic to the patients. Exploration of novel treatment approaches, such as gene therapy, is needed. Materials and Methods: The human salivary gland cancer cell line HSG was transiently transfected with full length human caspase-14 cDNA. Photomicroscopy, BrdU assay, cell counting, MTT assay, and TUNEL assay were applied. To determine the tumorigenicity, tumor volume, tumor pathology and vascularization were analyzed in vivo. Results: Cell growth and viability were inhibited significantly by transient caspase-14 expression. Caspase-14 expression resulted in a significant reduction of tumorigenicity. Importantly, a significant decrease in tumor blood vessel formation was observed. Conclusion: Salivary gland cancer cells underwent growth inhibition, cell death, and reduced tumorigenicity in vivo when exogenous caspase-14 was expressed, which could be due, in part, to an inhibitory effect of caspase-14 on tumor vascularization.
AB - Background: Current therapeutic approaches to salivary gland cancer are often associated with severe disfigurement and loss of glandular function, which are traumatic to the patients. Exploration of novel treatment approaches, such as gene therapy, is needed. Materials and Methods: The human salivary gland cancer cell line HSG was transiently transfected with full length human caspase-14 cDNA. Photomicroscopy, BrdU assay, cell counting, MTT assay, and TUNEL assay were applied. To determine the tumorigenicity, tumor volume, tumor pathology and vascularization were analyzed in vivo. Results: Cell growth and viability were inhibited significantly by transient caspase-14 expression. Caspase-14 expression resulted in a significant reduction of tumorigenicity. Importantly, a significant decrease in tumor blood vessel formation was observed. Conclusion: Salivary gland cancer cells underwent growth inhibition, cell death, and reduced tumorigenicity in vivo when exogenous caspase-14 was expressed, which could be due, in part, to an inhibitory effect of caspase-14 on tumor vascularization.
KW - Caspase-14
KW - Gene therapy
KW - Salivary gland cancer
KW - Vascularization
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M3 - Article
C2 - 19846913
AN - SCOPUS:71949125506
SN - 0250-7005
VL - 29
SP - 3811
EP - 3818
JO - Anticancer research
JF - Anticancer research
IS - 10
ER -