Exogenous L-arginine ameliorates angiotensin II-induced hypertension and renal damage in rats

Niwanthi W. Rajapakse, Carmen De Miguel, Satarupa Das, David L. Mattson

Research output: Contribution to journalArticle

Abstract

Experiments were performed to determine whether exogenous L-arginine could ameliorate angiotensin II-induced hypertension and renal damage. Rats were instrumented with chronic indwelling femoral venous and arterial catheters for infusions of drugs and measurement of conscious arterial pressure. Arterial blood pressure significantly increased from 124±1 to 199±4 mm Hg, after 9 days of continuous infusion of angiotensin II (20 ng/kg per minute; IV; n=6 to 9). In contrast, the increase in arterial pressure after 9 days of angiotensin II infusion was significantly blunted by 45% (P=0.0003) in rats coadministered L-arginine (300 μg/kg per minute; IV; n=7 to 9). The glomerular injury index was significantly greater in rats administered angiotensin II in comparison with rats administered saline vehicle (P<0.001). Coinfusion of L-arginine significantly increased plasma nitrate/nitrite concentrations (P<0.001) and completely prevented angiotensin II-induced glomerular damage (P<0.001). Angiotensin II infusion alone and combined angiotensin II plus L-arginine infusion significantly increased urinary albumin excretion. Albuminuria in rats administered angiotensin II plus L-arginine is likely to be because of increased intraglomerular pressure. Our experiments demonstrate that L-arginine can blunt angiotensin II-induced hypertension and associated renal damage. This latter observation is most exciting because it indicates that increasing NO bioavailability, in addition to lowering arterial pressure, can greatly reduce hypertension-induced renal damage.

Original languageEnglish (US)
Pages (from-to)1084-1090
Number of pages7
JournalHypertension
Volume52
Issue number6
DOIs
StatePublished - Dec 1 2008
Externally publishedYes

Fingerprint

Renal Hypertension
Angiotensin II
Arginine
Arterial Pressure
Albuminuria
Nitrites
Thigh
Nitrates
Biological Availability
Albumins
Catheters
Observation
Pressure
Wounds and Injuries

Keywords

  • Angiotensin II
  • Hypertension
  • Kidney
  • L-arginine
  • NO

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Exogenous L-arginine ameliorates angiotensin II-induced hypertension and renal damage in rats. / Rajapakse, Niwanthi W.; De Miguel, Carmen; Das, Satarupa; Mattson, David L.

In: Hypertension, Vol. 52, No. 6, 01.12.2008, p. 1084-1090.

Research output: Contribution to journalArticle

Rajapakse, Niwanthi W. ; De Miguel, Carmen ; Das, Satarupa ; Mattson, David L. / Exogenous L-arginine ameliorates angiotensin II-induced hypertension and renal damage in rats. In: Hypertension. 2008 ; Vol. 52, No. 6. pp. 1084-1090.
@article{98472f1681a34b75bf02e51e8bd99f3e,
title = "Exogenous L-arginine ameliorates angiotensin II-induced hypertension and renal damage in rats",
abstract = "Experiments were performed to determine whether exogenous L-arginine could ameliorate angiotensin II-induced hypertension and renal damage. Rats were instrumented with chronic indwelling femoral venous and arterial catheters for infusions of drugs and measurement of conscious arterial pressure. Arterial blood pressure significantly increased from 124±1 to 199±4 mm Hg, after 9 days of continuous infusion of angiotensin II (20 ng/kg per minute; IV; n=6 to 9). In contrast, the increase in arterial pressure after 9 days of angiotensin II infusion was significantly blunted by 45{\%} (P=0.0003) in rats coadministered L-arginine (300 μg/kg per minute; IV; n=7 to 9). The glomerular injury index was significantly greater in rats administered angiotensin II in comparison with rats administered saline vehicle (P<0.001). Coinfusion of L-arginine significantly increased plasma nitrate/nitrite concentrations (P<0.001) and completely prevented angiotensin II-induced glomerular damage (P<0.001). Angiotensin II infusion alone and combined angiotensin II plus L-arginine infusion significantly increased urinary albumin excretion. Albuminuria in rats administered angiotensin II plus L-arginine is likely to be because of increased intraglomerular pressure. Our experiments demonstrate that L-arginine can blunt angiotensin II-induced hypertension and associated renal damage. This latter observation is most exciting because it indicates that increasing NO bioavailability, in addition to lowering arterial pressure, can greatly reduce hypertension-induced renal damage.",
keywords = "Angiotensin II, Hypertension, Kidney, L-arginine, NO",
author = "Rajapakse, {Niwanthi W.} and {De Miguel}, Carmen and Satarupa Das and Mattson, {David L.}",
year = "2008",
month = "12",
day = "1",
doi = "10.1161/HYPERTENSIONAHA.108.114298",
language = "English (US)",
volume = "52",
pages = "1084--1090",
journal = "Hypertension",
issn = "0194-911X",
publisher = "Lippincott Williams and Wilkins",
number = "6",

}

TY - JOUR

T1 - Exogenous L-arginine ameliorates angiotensin II-induced hypertension and renal damage in rats

AU - Rajapakse, Niwanthi W.

AU - De Miguel, Carmen

AU - Das, Satarupa

AU - Mattson, David L.

PY - 2008/12/1

Y1 - 2008/12/1

N2 - Experiments were performed to determine whether exogenous L-arginine could ameliorate angiotensin II-induced hypertension and renal damage. Rats were instrumented with chronic indwelling femoral venous and arterial catheters for infusions of drugs and measurement of conscious arterial pressure. Arterial blood pressure significantly increased from 124±1 to 199±4 mm Hg, after 9 days of continuous infusion of angiotensin II (20 ng/kg per minute; IV; n=6 to 9). In contrast, the increase in arterial pressure after 9 days of angiotensin II infusion was significantly blunted by 45% (P=0.0003) in rats coadministered L-arginine (300 μg/kg per minute; IV; n=7 to 9). The glomerular injury index was significantly greater in rats administered angiotensin II in comparison with rats administered saline vehicle (P<0.001). Coinfusion of L-arginine significantly increased plasma nitrate/nitrite concentrations (P<0.001) and completely prevented angiotensin II-induced glomerular damage (P<0.001). Angiotensin II infusion alone and combined angiotensin II plus L-arginine infusion significantly increased urinary albumin excretion. Albuminuria in rats administered angiotensin II plus L-arginine is likely to be because of increased intraglomerular pressure. Our experiments demonstrate that L-arginine can blunt angiotensin II-induced hypertension and associated renal damage. This latter observation is most exciting because it indicates that increasing NO bioavailability, in addition to lowering arterial pressure, can greatly reduce hypertension-induced renal damage.

AB - Experiments were performed to determine whether exogenous L-arginine could ameliorate angiotensin II-induced hypertension and renal damage. Rats were instrumented with chronic indwelling femoral venous and arterial catheters for infusions of drugs and measurement of conscious arterial pressure. Arterial blood pressure significantly increased from 124±1 to 199±4 mm Hg, after 9 days of continuous infusion of angiotensin II (20 ng/kg per minute; IV; n=6 to 9). In contrast, the increase in arterial pressure after 9 days of angiotensin II infusion was significantly blunted by 45% (P=0.0003) in rats coadministered L-arginine (300 μg/kg per minute; IV; n=7 to 9). The glomerular injury index was significantly greater in rats administered angiotensin II in comparison with rats administered saline vehicle (P<0.001). Coinfusion of L-arginine significantly increased plasma nitrate/nitrite concentrations (P<0.001) and completely prevented angiotensin II-induced glomerular damage (P<0.001). Angiotensin II infusion alone and combined angiotensin II plus L-arginine infusion significantly increased urinary albumin excretion. Albuminuria in rats administered angiotensin II plus L-arginine is likely to be because of increased intraglomerular pressure. Our experiments demonstrate that L-arginine can blunt angiotensin II-induced hypertension and associated renal damage. This latter observation is most exciting because it indicates that increasing NO bioavailability, in addition to lowering arterial pressure, can greatly reduce hypertension-induced renal damage.

KW - Angiotensin II

KW - Hypertension

KW - Kidney

KW - L-arginine

KW - NO

UR - http://www.scopus.com/inward/record.url?scp=57449084343&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57449084343&partnerID=8YFLogxK

U2 - 10.1161/HYPERTENSIONAHA.108.114298

DO - 10.1161/HYPERTENSIONAHA.108.114298

M3 - Article

C2 - 18981330

AN - SCOPUS:57449084343

VL - 52

SP - 1084

EP - 1090

JO - Hypertension

JF - Hypertension

SN - 0194-911X

IS - 6

ER -