Exosome production and its regulation of EGFR during wound healing in renal tubular cells

Xiangjun Zhou, Wei Zhang, Qisheng Yao, Hao Zhang, Guie Dong, Ming Zhang, Yutao Liu, Jiankang Chen, Zheng Dong

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Kidney repair following injury involves the reconstitution of a structurally and functionally intact tubular epithelium. Growth factors and their receptors, such as EGFR, are important in the repair of renal tubules. Exosomes are cell-produced small (~100 nm in diameter) vesicles that contain and transfer proteins, lipids, RNAs, and DNAs between cells. In this study, we examined the relationship between exosome production and EGFR activation and the potential role of exosome in wound healing. EGFR activation occurred shortly after scratch wounding in renal tubular cells. Wound repair after scratching was significantly promoted by EGF and suppressed by EGFR inhibitor gefitinib. Interestingly, scratch wounding induced a significant increase of exosome production. The exosome production was decreased by EGF and increased by gefitinib, suggesting a suppressive role of EGFR signaling in exosome production. Conversely, inhibition of exosome release by GW4869 and manumycin A markedly increased EGFR activation and promoted wound healing. Moreover, exosomes derived from scratch-wounding cells could inhibit wound healing. Collectively, the results indicate that wound healing in renal tubular cells is associated with EGFR activation and exosome production. Although EGFR activation promotes wound healing, released exosomes may antagonize EGFR activation and wound healing.

Original languageEnglish (US)
Pages (from-to)F963-F970
JournalAmerican Journal of Physiology - Renal Physiology
Volume312
Issue number6
DOIs
StatePublished - Jan 1 2017

Fingerprint

Exosomes
Wound Healing
Kidney
Epidermal Growth Factor
Growth Factor Receptors
Wounds and Injuries
Epithelium
RNA

Keywords

  • EGFR
  • Exosomes
  • Kidney
  • Wound healing

ASJC Scopus subject areas

  • Physiology
  • Urology

Cite this

Exosome production and its regulation of EGFR during wound healing in renal tubular cells. / Zhou, Xiangjun; Zhang, Wei; Yao, Qisheng; Zhang, Hao; Dong, Guie; Zhang, Ming; Liu, Yutao; Chen, Jiankang; Dong, Zheng.

In: American Journal of Physiology - Renal Physiology, Vol. 312, No. 6, 01.01.2017, p. F963-F970.

Research output: Contribution to journalArticle

@article{2bebedb86fb5402cae6737fd2806989c,
title = "Exosome production and its regulation of EGFR during wound healing in renal tubular cells",
abstract = "Kidney repair following injury involves the reconstitution of a structurally and functionally intact tubular epithelium. Growth factors and their receptors, such as EGFR, are important in the repair of renal tubules. Exosomes are cell-produced small (~100 nm in diameter) vesicles that contain and transfer proteins, lipids, RNAs, and DNAs between cells. In this study, we examined the relationship between exosome production and EGFR activation and the potential role of exosome in wound healing. EGFR activation occurred shortly after scratch wounding in renal tubular cells. Wound repair after scratching was significantly promoted by EGF and suppressed by EGFR inhibitor gefitinib. Interestingly, scratch wounding induced a significant increase of exosome production. The exosome production was decreased by EGF and increased by gefitinib, suggesting a suppressive role of EGFR signaling in exosome production. Conversely, inhibition of exosome release by GW4869 and manumycin A markedly increased EGFR activation and promoted wound healing. Moreover, exosomes derived from scratch-wounding cells could inhibit wound healing. Collectively, the results indicate that wound healing in renal tubular cells is associated with EGFR activation and exosome production. Although EGFR activation promotes wound healing, released exosomes may antagonize EGFR activation and wound healing.",
keywords = "EGFR, Exosomes, Kidney, Wound healing",
author = "Xiangjun Zhou and Wei Zhang and Qisheng Yao and Hao Zhang and Guie Dong and Ming Zhang and Yutao Liu and Jiankang Chen and Zheng Dong",
year = "2017",
month = "1",
day = "1",
doi = "10.1152/ajprenal.00078.2017",
language = "English (US)",
volume = "312",
pages = "F963--F970",
journal = "American Journal of Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "6",

}

TY - JOUR

T1 - Exosome production and its regulation of EGFR during wound healing in renal tubular cells

AU - Zhou, Xiangjun

AU - Zhang, Wei

AU - Yao, Qisheng

AU - Zhang, Hao

AU - Dong, Guie

AU - Zhang, Ming

AU - Liu, Yutao

AU - Chen, Jiankang

AU - Dong, Zheng

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Kidney repair following injury involves the reconstitution of a structurally and functionally intact tubular epithelium. Growth factors and their receptors, such as EGFR, are important in the repair of renal tubules. Exosomes are cell-produced small (~100 nm in diameter) vesicles that contain and transfer proteins, lipids, RNAs, and DNAs between cells. In this study, we examined the relationship between exosome production and EGFR activation and the potential role of exosome in wound healing. EGFR activation occurred shortly after scratch wounding in renal tubular cells. Wound repair after scratching was significantly promoted by EGF and suppressed by EGFR inhibitor gefitinib. Interestingly, scratch wounding induced a significant increase of exosome production. The exosome production was decreased by EGF and increased by gefitinib, suggesting a suppressive role of EGFR signaling in exosome production. Conversely, inhibition of exosome release by GW4869 and manumycin A markedly increased EGFR activation and promoted wound healing. Moreover, exosomes derived from scratch-wounding cells could inhibit wound healing. Collectively, the results indicate that wound healing in renal tubular cells is associated with EGFR activation and exosome production. Although EGFR activation promotes wound healing, released exosomes may antagonize EGFR activation and wound healing.

AB - Kidney repair following injury involves the reconstitution of a structurally and functionally intact tubular epithelium. Growth factors and their receptors, such as EGFR, are important in the repair of renal tubules. Exosomes are cell-produced small (~100 nm in diameter) vesicles that contain and transfer proteins, lipids, RNAs, and DNAs between cells. In this study, we examined the relationship between exosome production and EGFR activation and the potential role of exosome in wound healing. EGFR activation occurred shortly after scratch wounding in renal tubular cells. Wound repair after scratching was significantly promoted by EGF and suppressed by EGFR inhibitor gefitinib. Interestingly, scratch wounding induced a significant increase of exosome production. The exosome production was decreased by EGF and increased by gefitinib, suggesting a suppressive role of EGFR signaling in exosome production. Conversely, inhibition of exosome release by GW4869 and manumycin A markedly increased EGFR activation and promoted wound healing. Moreover, exosomes derived from scratch-wounding cells could inhibit wound healing. Collectively, the results indicate that wound healing in renal tubular cells is associated with EGFR activation and exosome production. Although EGFR activation promotes wound healing, released exosomes may antagonize EGFR activation and wound healing.

KW - EGFR

KW - Exosomes

KW - Kidney

KW - Wound healing

UR - http://www.scopus.com/inward/record.url?scp=85020076813&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85020076813&partnerID=8YFLogxK

U2 - 10.1152/ajprenal.00078.2017

DO - 10.1152/ajprenal.00078.2017

M3 - Article

C2 - 28356285

AN - SCOPUS:85020076813

VL - 312

SP - F963-F970

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 1931-857X

IS - 6

ER -