Expanded cohorts of maternal CD8+ T-cells specific for paternal MHC class I accumulate during pregnancy

Min Zhou, Andrew L. Mellor

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

A recombinant H-2Kb transgene, GK, containing the human HLA-G gene promoter is expressed throughout the trophoblast when inherited paternally. Male GK transgenic mice were mated with female T-cell receptor (TCR) transgenic mice to assess the effect of fetal H-2Kb expression on maternal H-2Kb-specific CD8+ T-cells during pregnancy. The number of maternal H- 2Kb-specific CD8+ T-cells in spleen increased significantly (~ 3-fold) 10 days post coitus when the GK transgene was inherited from the father. A smaller (~ 2-fold) increase was observed in the spleen of pregnant females mated with C57BL/10 (H-2b) males. No increase was observed in mothers mated to syngeneic male mice. In both cases where expanded cohorts of maternal CD8+ T-cells were observed the amount of surface CD8 and to a lesser extent, TCR molecules was reduced. No change in the amount of surface CD44 or CD45RB was detected when levels were compared with naive T-cells from control virgin female mice. Expanded cohorts of CD8+ T-cells were also detected in para- aortic and inguinal lymph nodes draining the uterus but no changes were observed in mesenteric lymph nodes. This study concludes that maternal CD8+ T-cells are exposed to paternally inherited fetal MHC class I antigens during pregnancy. Moreover, the phenotype of the CD8+ T-cells in maternal spleen and lymph nodes that drain the uterus is not typical of activated, antigen- experienced T-cells suggesting that contact with fetal H-2Kb molecules induces a state of functional unresponsiveness.

Original languageEnglish (US)
Pages (from-to)47-62
Number of pages16
JournalJournal of Reproductive Immunology
Volume40
Issue number1
DOIs
StatePublished - Oct 1 1998

Fingerprint

Mothers
T-Lymphocytes
Pregnancy
Spleen
Lymph Nodes
T-Cell Antigen Receptor
Transgenes
Transgenic Mice
Uterus
HLA-G Antigens
Histocompatibility Antigens Class I
Groin
Coitus
Trophoblasts
Fathers
Phenotype
Antigens
Genes

Keywords

  • MHC I
  • Pregnancy
  • T-cells
  • Trophoblast

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

Expanded cohorts of maternal CD8+ T-cells specific for paternal MHC class I accumulate during pregnancy. / Zhou, Min; Mellor, Andrew L.

In: Journal of Reproductive Immunology, Vol. 40, No. 1, 01.10.1998, p. 47-62.

Research output: Contribution to journalArticle

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