Experimental metastasis and CTL adoptive transfer immunotherapy mouse model

Mary Zimmerman, Xiaolin Hu, Kebin Liu

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Experimental metastasis mouse model is a simple and yet physiologically relevant metastasis model. The tumor cells are injected intravenously (i.v) into mouse tail veins and colonize in the lungs, thereby, resembling the last steps of tumor cell spontaneous metastasis: survival in the circulation, extravasation and colonization in the distal organs. From a therapeutic point of view, the experimental metastasis model is the simplest and ideal model since the target of therapies is often the end point of metastasis: established metastatic tumor in the distal organ. In this model, tumor cells are injected i.v into mouse tail veins and allowed to colonize and grow in the lungs. Tumor-specific CTLs are then injected i.v into the metastases-bearing mouse. The number and size of the lung metastases can be controlled by the number of tumor cells to be injected and the time of tumor growth. Therefore, various stages of metastasis, from minimal metastasis to extensive metastasis, can be modeled. Lung metastases are analyzed by inflation with ink, thus allowing easier visual observation and quantification.

Original languageEnglish (US)
Article numbere2077
JournalJournal of Visualized Experiments
Issue number45
DOIs
StatePublished - Nov 1 2010

Keywords

  • CTL adoptive transfer
  • Immunology
  • Issue 45
  • Lung
  • Metastasis
  • Tumor Immunology

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemical Engineering(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

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