Experimental murine invasive pulmonary aspergillosis

D. J. Eisenstein, P. W. Biddinger, J. C. Rhodes

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

A new model of invasive pulmonary aspergillosis (IPA) was developed in immunosuppressed mice. Intranasal route of inoculation was used to deliver predictable numbers of Aspergillus flavus conidia. The LD50 was determined to be 2.7 x 102 viable conidia, and a combination of quantitative culture and determination of chitin content was shown to best measure the progression of pulmonary disease. The evolution of IPA in these mice conformed with what has been reported in human cases of aspergillosis; both histopathology of the pulmonary lesions and dissemination pattern resembled their human counterparts. The authors hope to use this model to study virulence mechanisms of Aspergillus and novel therapeutic methods.

Original languageEnglish (US)
Pages (from-to)510-515
Number of pages6
JournalAmerican Journal of Clinical Pathology
Volume93
Issue number4
DOIs
StatePublished - Jan 1 1990

Keywords

  • Aspergillosis
  • Histopathology
  • Intranasal inoculations
  • LD50
  • Murine model

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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