Experimental studies on in vitro maturation of porcine oocytes. II. Effects of estradiol-17 beta and progesterone.

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Abstract

The simple compounded semi-defined Whitten's medium was supplemented with estradiol-17 beta and progesterone to examine whether they influence the nuclear maturation of porcine cumulus-oocyte complexes. Addition of estradiol-17 beta at concentrations of 1.0 microgram/ml and 10.0 micrograms/ml significantly inhibited the nuclear maturation in comparison to the control group. No significant inhibition of estradiol-17 beta was observed at concentration of 0.1 microgram/ml with respect to the control. After addition of synchronous or subsequent (12 h after the incubation with estradiol-17 beta) supplementation of progesterone at concentration of 1 microgram/ml, maturation rates were as high as control. So progesterone overcame the inhibitory effect of estradiol-17 beta.

Original languageEnglish (US)
Pages (from-to)157-159
Number of pages3
JournalBerliner und Münchener tierärztliche Wochenschrift
Volume106
Issue number5
StatePublished - May 1993
Externally publishedYes

ASJC Scopus subject areas

  • veterinary(all)

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