Experimental use of an acrolein-based primer as collagen cross-linker for dentine bonding

Tatjana Maravic, Lorenzo Breschi, Allegra Comba, Sandra Ribeiro Cunha, Valeria Angeloni, Cesare Nucci, Josimeri Hebling, David Pashley, Franklin Tay, Annalisa Mazzoni

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objectives The objective of the present study was to investigate the long-term effect of 0.01% acrolein (ACR) aqueous solution, employed as an additional primer, on the mechanical durability and enzymatic activity of resin-dentine interfaces created with a simplified etch-and-rinse adhesive. Methods Dentine surfaces were etched with 35% phosphoric acid for 15 s, rinsed and blot-dried. Specimens were then assigned to: Group 1: dentine pre-treated with 0.01% ACR aqueous solution for 1 min and bonded with Adper Scotchbond 1 XT (SB1XT), a 2-step etch-and-rinse adhesive; Group 2: SB1XT was applied on untreated acid-etched dentine (control). Resin composite build-ups were made using Filtek Z250. Microtensile bond strength was tested by stressing sectioned specimens to failure immediately or after 1 year of storage in artificial saliva at 37 °C. Zymography and in-situ zymography assays were performed for examining dentine matrix metalloproteinase (MMP) activities. Results The use of 0.01% ACR as conditioning primer appeared to have contributed better to preservation of bond strength over time without affecting immediate bond strength. Zymography and in-situ zymography showed reduction in MMP activities after the application of ACR. Conclusion Dentine collagen cross-linking produced by an ACR-based primer increases the longevity of resin-dentine bonds by reinforcement of the adhesive interface and reduction of dentine MMP activities. Further studies are required to evaluate the potential in vivo and in vivo cytotoxicity of ACR. Clinical significance The acrolein-based primer is a potentially useful clinical bonding tool because it demonstrates good collagen cross-linking ability within a clinically-acceptable working time. Although a low ACR concentration was employed in the present study, the cytotoxicity of ACR should be tested prior to clinical use.

Original languageEnglish (US)
Pages (from-to)85-90
Number of pages6
JournalJournal of Dentistry
Volume68
DOIs
StatePublished - Jan 2018

Fingerprint

Acrolein
Dentin
Collagen
Matrix Metalloproteinases
Adhesives
Artificial Saliva
Composite Resins

Keywords

  • Acrolein
  • Collagen cross-linkers
  • Hybrid layer durability
  • Matrix metalloproteinases

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Maravic, T., Breschi, L., Comba, A., Cunha, S. R., Angeloni, V., Nucci, C., ... Mazzoni, A. (2018). Experimental use of an acrolein-based primer as collagen cross-linker for dentine bonding. Journal of Dentistry, 68, 85-90. https://doi.org/10.1016/j.jdent.2017.11.006

Experimental use of an acrolein-based primer as collagen cross-linker for dentine bonding. / Maravic, Tatjana; Breschi, Lorenzo; Comba, Allegra; Cunha, Sandra Ribeiro; Angeloni, Valeria; Nucci, Cesare; Hebling, Josimeri; Pashley, David; Tay, Franklin; Mazzoni, Annalisa.

In: Journal of Dentistry, Vol. 68, 01.2018, p. 85-90.

Research output: Contribution to journalArticle

Maravic, T, Breschi, L, Comba, A, Cunha, SR, Angeloni, V, Nucci, C, Hebling, J, Pashley, D, Tay, F & Mazzoni, A 2018, 'Experimental use of an acrolein-based primer as collagen cross-linker for dentine bonding', Journal of Dentistry, vol. 68, pp. 85-90. https://doi.org/10.1016/j.jdent.2017.11.006
Maravic, Tatjana ; Breschi, Lorenzo ; Comba, Allegra ; Cunha, Sandra Ribeiro ; Angeloni, Valeria ; Nucci, Cesare ; Hebling, Josimeri ; Pashley, David ; Tay, Franklin ; Mazzoni, Annalisa. / Experimental use of an acrolein-based primer as collagen cross-linker for dentine bonding. In: Journal of Dentistry. 2018 ; Vol. 68. pp. 85-90.
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abstract = "Objectives The objective of the present study was to investigate the long-term effect of 0.01{\%} acrolein (ACR) aqueous solution, employed as an additional primer, on the mechanical durability and enzymatic activity of resin-dentine interfaces created with a simplified etch-and-rinse adhesive. Methods Dentine surfaces were etched with 35{\%} phosphoric acid for 15 s, rinsed and blot-dried. Specimens were then assigned to: Group 1: dentine pre-treated with 0.01{\%} ACR aqueous solution for 1 min and bonded with Adper Scotchbond 1 XT (SB1XT), a 2-step etch-and-rinse adhesive; Group 2: SB1XT was applied on untreated acid-etched dentine (control). Resin composite build-ups were made using Filtek Z250. Microtensile bond strength was tested by stressing sectioned specimens to failure immediately or after 1 year of storage in artificial saliva at 37 °C. Zymography and in-situ zymography assays were performed for examining dentine matrix metalloproteinase (MMP) activities. Results The use of 0.01{\%} ACR as conditioning primer appeared to have contributed better to preservation of bond strength over time without affecting immediate bond strength. Zymography and in-situ zymography showed reduction in MMP activities after the application of ACR. Conclusion Dentine collagen cross-linking produced by an ACR-based primer increases the longevity of resin-dentine bonds by reinforcement of the adhesive interface and reduction of dentine MMP activities. Further studies are required to evaluate the potential in vivo and in vivo cytotoxicity of ACR. Clinical significance The acrolein-based primer is a potentially useful clinical bonding tool because it demonstrates good collagen cross-linking ability within a clinically-acceptable working time. Although a low ACR concentration was employed in the present study, the cytotoxicity of ACR should be tested prior to clinical use.",
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AU - Maravic, Tatjana

AU - Breschi, Lorenzo

AU - Comba, Allegra

AU - Cunha, Sandra Ribeiro

AU - Angeloni, Valeria

AU - Nucci, Cesare

AU - Hebling, Josimeri

AU - Pashley, David

AU - Tay, Franklin

AU - Mazzoni, Annalisa

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N2 - Objectives The objective of the present study was to investigate the long-term effect of 0.01% acrolein (ACR) aqueous solution, employed as an additional primer, on the mechanical durability and enzymatic activity of resin-dentine interfaces created with a simplified etch-and-rinse adhesive. Methods Dentine surfaces were etched with 35% phosphoric acid for 15 s, rinsed and blot-dried. Specimens were then assigned to: Group 1: dentine pre-treated with 0.01% ACR aqueous solution for 1 min and bonded with Adper Scotchbond 1 XT (SB1XT), a 2-step etch-and-rinse adhesive; Group 2: SB1XT was applied on untreated acid-etched dentine (control). Resin composite build-ups were made using Filtek Z250. Microtensile bond strength was tested by stressing sectioned specimens to failure immediately or after 1 year of storage in artificial saliva at 37 °C. Zymography and in-situ zymography assays were performed for examining dentine matrix metalloproteinase (MMP) activities. Results The use of 0.01% ACR as conditioning primer appeared to have contributed better to preservation of bond strength over time without affecting immediate bond strength. Zymography and in-situ zymography showed reduction in MMP activities after the application of ACR. Conclusion Dentine collagen cross-linking produced by an ACR-based primer increases the longevity of resin-dentine bonds by reinforcement of the adhesive interface and reduction of dentine MMP activities. Further studies are required to evaluate the potential in vivo and in vivo cytotoxicity of ACR. Clinical significance The acrolein-based primer is a potentially useful clinical bonding tool because it demonstrates good collagen cross-linking ability within a clinically-acceptable working time. Although a low ACR concentration was employed in the present study, the cytotoxicity of ACR should be tested prior to clinical use.

AB - Objectives The objective of the present study was to investigate the long-term effect of 0.01% acrolein (ACR) aqueous solution, employed as an additional primer, on the mechanical durability and enzymatic activity of resin-dentine interfaces created with a simplified etch-and-rinse adhesive. Methods Dentine surfaces were etched with 35% phosphoric acid for 15 s, rinsed and blot-dried. Specimens were then assigned to: Group 1: dentine pre-treated with 0.01% ACR aqueous solution for 1 min and bonded with Adper Scotchbond 1 XT (SB1XT), a 2-step etch-and-rinse adhesive; Group 2: SB1XT was applied on untreated acid-etched dentine (control). Resin composite build-ups were made using Filtek Z250. Microtensile bond strength was tested by stressing sectioned specimens to failure immediately or after 1 year of storage in artificial saliva at 37 °C. Zymography and in-situ zymography assays were performed for examining dentine matrix metalloproteinase (MMP) activities. Results The use of 0.01% ACR as conditioning primer appeared to have contributed better to preservation of bond strength over time without affecting immediate bond strength. Zymography and in-situ zymography showed reduction in MMP activities after the application of ACR. Conclusion Dentine collagen cross-linking produced by an ACR-based primer increases the longevity of resin-dentine bonds by reinforcement of the adhesive interface and reduction of dentine MMP activities. Further studies are required to evaluate the potential in vivo and in vivo cytotoxicity of ACR. Clinical significance The acrolein-based primer is a potentially useful clinical bonding tool because it demonstrates good collagen cross-linking ability within a clinically-acceptable working time. Although a low ACR concentration was employed in the present study, the cytotoxicity of ACR should be tested prior to clinical use.

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KW - Hybrid layer durability

KW - Matrix metalloproteinases

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