Expressed CpG island sequence tag microarray for dual screening of DNA hypermethylation and gene silencing in cancer cells

Huidong Shi, Pearlly S. Yan, Farahnaz Rahmatpanah, Charles W. Caldwell, Tim Hui Ming Huang, Chuan Mu Chen, Catherine Lofton-Day

Research output: Contribution to journalArticle

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Abstract

We present a novel concept by using expressed CpG island sequence tags (ECISTs) for dual analysis of DNA methylation and gene expression in cancer cells. ECISTs are present in the genome and are DNA fragments expected to be located in the promoter and first exon region of genes. Their GC-rich segments can be used for screening hypermethylated CpG sites in cancer cells, and their exon-containing portions can be used for measuring levels of the corresponding transcripts. A total of 1162 loci met the criteria of ECISTs from an initial screening of 7776 CpG island tags. This ECIST panel was used to analyze the breast cancer cell line MDA-MB-231, which was treated with a demethylating agent. Microarray profile analysis identified 30 methylation-silenced genes, the expression of which could be directly reactivated by demethylation. An additional group of 96 up-regulated genes was also identified but appeared to be down-stream from this epigenetic cascade. Thus, this study shows that the ECIST microarray can be used to differentiate the primary and secondary causes of demethylation and provides an effective tool to elucidate the mechanisms of aberrant DNA methylation in cancer.

Original languageEnglish (US)
Pages (from-to)3214-3220
Number of pages7
JournalCancer Research
Volume62
Issue number11
StatePublished - Jun 1 2002
Externally publishedYes

Fingerprint

CpG Islands
Gene Silencing
DNA
Neoplasms
DNA Methylation
Exons
Gene Expression
Microarray Analysis
Epigenomics
Methylation
Genes
Genome
Breast Neoplasms
Cell Line

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Shi, H., Yan, P. S., Rahmatpanah, F., Caldwell, C. W., Huang, T. H. M., Chen, C. M., & Lofton-Day, C. (2002). Expressed CpG island sequence tag microarray for dual screening of DNA hypermethylation and gene silencing in cancer cells. Cancer Research, 62(11), 3214-3220.

Expressed CpG island sequence tag microarray for dual screening of DNA hypermethylation and gene silencing in cancer cells. / Shi, Huidong; Yan, Pearlly S.; Rahmatpanah, Farahnaz; Caldwell, Charles W.; Huang, Tim Hui Ming; Chen, Chuan Mu; Lofton-Day, Catherine.

In: Cancer Research, Vol. 62, No. 11, 01.06.2002, p. 3214-3220.

Research output: Contribution to journalArticle

Shi, H, Yan, PS, Rahmatpanah, F, Caldwell, CW, Huang, THM, Chen, CM & Lofton-Day, C 2002, 'Expressed CpG island sequence tag microarray for dual screening of DNA hypermethylation and gene silencing in cancer cells', Cancer Research, vol. 62, no. 11, pp. 3214-3220.
Shi H, Yan PS, Rahmatpanah F, Caldwell CW, Huang THM, Chen CM et al. Expressed CpG island sequence tag microarray for dual screening of DNA hypermethylation and gene silencing in cancer cells. Cancer Research. 2002 Jun 1;62(11):3214-3220.
Shi, Huidong ; Yan, Pearlly S. ; Rahmatpanah, Farahnaz ; Caldwell, Charles W. ; Huang, Tim Hui Ming ; Chen, Chuan Mu ; Lofton-Day, Catherine. / Expressed CpG island sequence tag microarray for dual screening of DNA hypermethylation and gene silencing in cancer cells. In: Cancer Research. 2002 ; Vol. 62, No. 11. pp. 3214-3220.
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