Expression and alternative splicing of the mouse autoimmune regulator gene (Aire)

Qing Guo Ruan, Cong Yi Wang, Jing Da Shi, Jin-Xiong She

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

AIRE, the gene responsible for the autoimmune polyglandular syndrome type 1 (APS1) or APECED, may act as a transcription factor according to its predicated protein structure. Here we demonstrate the low expression level of the mouse Aire gene as it is undetectable by Northern blot analyses. However, RT-PCR analyses revealed expression of Aire in mouse thymus, ovary, lung, testis, kidney and adrenal gland. Barely detectable level of RT-PCR product was also found in thyroid gland and heart but no amplification was detected in pancreas, spleen and liver. Competitive RT-PCR assays demonstrated highest expression level of Aire mRNA in thymus. In addition to the complete cDNA (Aire or Aire-1a), we identified 11 alternative splicing forms (designated as Aire-1b, Aire-1c, Aire-1d, Aire-2a, Aire-2b, Aire-2c, Aire-2d, Aire-3a, Aire- 3b, Aire-3c and Aire-3d). These forms result from combinations of four alternative splicing units (exon 10, exon 11, 12 bp in exon 6 and 3 bp in exon 8). The relative abundance of these splicing forms was also determined.

Original languageEnglish (US)
Pages (from-to)307-313
Number of pages7
JournalJournal of Autoimmunity
Volume13
Issue number3
DOIs
StatePublished - Jan 1 1999
Externally publishedYes

Fingerprint

Alternative Splicing
Regulator Genes
Exons
Autoimmune Polyendocrinopathies
Polymerase Chain Reaction
Thymus Gland
Adrenal Glands
Northern Blotting
Genes

Keywords

  • APECED
  • APS
  • Alternative splicing
  • Autoimmunity
  • Expression

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Expression and alternative splicing of the mouse autoimmune regulator gene (Aire). / Ruan, Qing Guo; Wang, Cong Yi; Shi, Jing Da; She, Jin-Xiong.

In: Journal of Autoimmunity, Vol. 13, No. 3, 01.01.1999, p. 307-313.

Research output: Contribution to journalArticle

Ruan, Qing Guo ; Wang, Cong Yi ; Shi, Jing Da ; She, Jin-Xiong. / Expression and alternative splicing of the mouse autoimmune regulator gene (Aire). In: Journal of Autoimmunity. 1999 ; Vol. 13, No. 3. pp. 307-313.
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