Expression and polarized localization of the hemochromatosis gene product HFE in retinal pigment epithelium

Pamela M. Martin, Jaya P. Gnana-Prakasam, Penny Roon, Robert G. Smith, Sylvia B. Smith, Vadivel Ganapathy

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

PURPOSE. Hereditary hemochromatosis is an autosomal recessive disorder of iron overload leading to oxidative stress. Mutations in HFE are responsible for ∼90% of cases of this disease. HFE is the principal regulator of iron homeostasis, and the process involves interaction with transferrin receptor (TfR)-1, transferrin receptor (TfR)-2, and β2-microglobulin (β2M). Expression of HFE has not been investigated in the retina. In the present study, the expression of HFE and the HFE-interacting proteins TfR1, TfR2, and β2M were analyzed in mouse retina. METHODS. RT-PCR was used to detect the expression of HFE mRNA in neural retina and the RPE-eyecup. Expression of HFE in intact retina was investigated by in situ hybridization, immunofluorescence, and immunogold electron microscopy. Expression of HFE-interacting proteins was also analyzed using similar techniques. RESULTS. RT-PCR showed predominant expression of HFE mRNA in the RPE-eyecup. In situ hybridization in intact retina revealed that HFE mRNA is expressed almost exclusively in RPE Immunofluorescence and immunogold electron microscopy showed that HFE protein was specifically associated with the basolateral membrane of RPE. Expression of the HFE-interacting proteins TfR1, TfR2, and β2M was also evident in the retina. CONCLUSIONS. This is the first report on the expression of HFE in the retina. The specific localization of HFE and its interacting proteins, TfR1 and TfR2, at the basolateral membrane of RPE is relevant to the regulation of iron homeostasis in this cell. Patients with hemochromatosis may have impairment of iron homeostasis in RPE, potentially contributing to age-related RPE dysfunction and retinal degeneration.

Original languageEnglish (US)
Pages (from-to)4238-4244
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume47
Issue number10
DOIs
StatePublished - Oct 1 2006

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Hemochromatosis
Retinal Pigment Epithelium
Retina
Genes
Transferrin Receptors
Homeostasis
Iron
Messenger RNA
In Situ Hybridization
Fluorescent Antibody Technique
Electron Microscopy
Polymerase Chain Reaction
Retinal Degeneration
Iron Overload
Membranes
Oxidative Stress
Mutation
Hemochromatosis Protein

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Expression and polarized localization of the hemochromatosis gene product HFE in retinal pigment epithelium. / Martin, Pamela M.; Gnana-Prakasam, Jaya P.; Roon, Penny; Smith, Robert G.; Smith, Sylvia B.; Ganapathy, Vadivel.

In: Investigative Ophthalmology and Visual Science, Vol. 47, No. 10, 01.10.2006, p. 4238-4244.

Research output: Contribution to journalArticle

Martin, Pamela M. ; Gnana-Prakasam, Jaya P. ; Roon, Penny ; Smith, Robert G. ; Smith, Sylvia B. ; Ganapathy, Vadivel. / Expression and polarized localization of the hemochromatosis gene product HFE in retinal pigment epithelium. In: Investigative Ophthalmology and Visual Science. 2006 ; Vol. 47, No. 10. pp. 4238-4244.
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abstract = "PURPOSE. Hereditary hemochromatosis is an autosomal recessive disorder of iron overload leading to oxidative stress. Mutations in HFE are responsible for ∼90{\%} of cases of this disease. HFE is the principal regulator of iron homeostasis, and the process involves interaction with transferrin receptor (TfR)-1, transferrin receptor (TfR)-2, and β2-microglobulin (β2M). Expression of HFE has not been investigated in the retina. In the present study, the expression of HFE and the HFE-interacting proteins TfR1, TfR2, and β2M were analyzed in mouse retina. METHODS. RT-PCR was used to detect the expression of HFE mRNA in neural retina and the RPE-eyecup. Expression of HFE in intact retina was investigated by in situ hybridization, immunofluorescence, and immunogold electron microscopy. Expression of HFE-interacting proteins was also analyzed using similar techniques. RESULTS. RT-PCR showed predominant expression of HFE mRNA in the RPE-eyecup. In situ hybridization in intact retina revealed that HFE mRNA is expressed almost exclusively in RPE Immunofluorescence and immunogold electron microscopy showed that HFE protein was specifically associated with the basolateral membrane of RPE. Expression of the HFE-interacting proteins TfR1, TfR2, and β2M was also evident in the retina. CONCLUSIONS. This is the first report on the expression of HFE in the retina. The specific localization of HFE and its interacting proteins, TfR1 and TfR2, at the basolateral membrane of RPE is relevant to the regulation of iron homeostasis in this cell. Patients with hemochromatosis may have impairment of iron homeostasis in RPE, potentially contributing to age-related RPE dysfunction and retinal degeneration.",
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AU - Smith, Sylvia B.

AU - Ganapathy, Vadivel

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N2 - PURPOSE. Hereditary hemochromatosis is an autosomal recessive disorder of iron overload leading to oxidative stress. Mutations in HFE are responsible for ∼90% of cases of this disease. HFE is the principal regulator of iron homeostasis, and the process involves interaction with transferrin receptor (TfR)-1, transferrin receptor (TfR)-2, and β2-microglobulin (β2M). Expression of HFE has not been investigated in the retina. In the present study, the expression of HFE and the HFE-interacting proteins TfR1, TfR2, and β2M were analyzed in mouse retina. METHODS. RT-PCR was used to detect the expression of HFE mRNA in neural retina and the RPE-eyecup. Expression of HFE in intact retina was investigated by in situ hybridization, immunofluorescence, and immunogold electron microscopy. Expression of HFE-interacting proteins was also analyzed using similar techniques. RESULTS. RT-PCR showed predominant expression of HFE mRNA in the RPE-eyecup. In situ hybridization in intact retina revealed that HFE mRNA is expressed almost exclusively in RPE Immunofluorescence and immunogold electron microscopy showed that HFE protein was specifically associated with the basolateral membrane of RPE. Expression of the HFE-interacting proteins TfR1, TfR2, and β2M was also evident in the retina. CONCLUSIONS. This is the first report on the expression of HFE in the retina. The specific localization of HFE and its interacting proteins, TfR1 and TfR2, at the basolateral membrane of RPE is relevant to the regulation of iron homeostasis in this cell. Patients with hemochromatosis may have impairment of iron homeostasis in RPE, potentially contributing to age-related RPE dysfunction and retinal degeneration.

AB - PURPOSE. Hereditary hemochromatosis is an autosomal recessive disorder of iron overload leading to oxidative stress. Mutations in HFE are responsible for ∼90% of cases of this disease. HFE is the principal regulator of iron homeostasis, and the process involves interaction with transferrin receptor (TfR)-1, transferrin receptor (TfR)-2, and β2-microglobulin (β2M). Expression of HFE has not been investigated in the retina. In the present study, the expression of HFE and the HFE-interacting proteins TfR1, TfR2, and β2M were analyzed in mouse retina. METHODS. RT-PCR was used to detect the expression of HFE mRNA in neural retina and the RPE-eyecup. Expression of HFE in intact retina was investigated by in situ hybridization, immunofluorescence, and immunogold electron microscopy. Expression of HFE-interacting proteins was also analyzed using similar techniques. RESULTS. RT-PCR showed predominant expression of HFE mRNA in the RPE-eyecup. In situ hybridization in intact retina revealed that HFE mRNA is expressed almost exclusively in RPE Immunofluorescence and immunogold electron microscopy showed that HFE protein was specifically associated with the basolateral membrane of RPE. Expression of the HFE-interacting proteins TfR1, TfR2, and β2M was also evident in the retina. CONCLUSIONS. This is the first report on the expression of HFE in the retina. The specific localization of HFE and its interacting proteins, TfR1 and TfR2, at the basolateral membrane of RPE is relevant to the regulation of iron homeostasis in this cell. Patients with hemochromatosis may have impairment of iron homeostasis in RPE, potentially contributing to age-related RPE dysfunction and retinal degeneration.

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