Expression characteristics and relevance of sodium glucose cotransporter-1 in mammalian renal tubulogenesis

Qiwei Yang, Yufeng Tian, Jun Wada, Naoki Kashihara, Elisabeth Wallner, Darryl Peterson, Yashpal S. Kanwar

Research output: Contribution to journalArticle

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Abstract

Expression and role of sodium glucose cotransporter (SGLT-1) in tubulogenesis were investigated during renal development. A mouse SGLT-1 cDNA was cloned, and it had substantial homology with human and rat forms. Four mRNA transcripts were detected, which differed in size from other species. SGLT-1 transcripts were detected at day 13 of gestation, and their expression increased during later stages extending into the postnatal period. A high mRNA and protein expression of SGLT-1 was seen in tubular segments of the inner cortex and outer medulla at day 16, and it was developmentally regulated. Treatment with SGLT-1 antisense selectively decreased the population of tubules in the metanephric explants. Expression of glomerular mRNA and WGA binding were unchanged. SGLT-1 activity, as measured by [14C]methyl-α-D-glucopyranoside uptake, increased during gestation in the tubular segments where it is expressed. Glucose uptake was inhibited by the treatment with SGLT-1 antisense and D-galactose. The data suggest that SGLT-1 exhibits a restricted spatiotemporal expression with functional activity confined to the corresponding tubular segments, and it selectively maintains renal tubulogenesis during development.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume279
Issue number4 48-4
StatePublished - Dec 7 2000

Fingerprint

Sodium-Glucose Transporter 1
Kidney
Messenger RNA
Sodium-Glucose Transport Proteins
Pregnancy
Galactose
Complementary DNA
Glucose
Population

Keywords

  • Renal development

ASJC Scopus subject areas

  • Physiology
  • Urology

Cite this

Yang, Q., Tian, Y., Wada, J., Kashihara, N., Wallner, E., Peterson, D., & Kanwar, Y. S. (2000). Expression characteristics and relevance of sodium glucose cotransporter-1 in mammalian renal tubulogenesis. American Journal of Physiology - Renal Physiology, 279(4 48-4).

Expression characteristics and relevance of sodium glucose cotransporter-1 in mammalian renal tubulogenesis. / Yang, Qiwei; Tian, Yufeng; Wada, Jun; Kashihara, Naoki; Wallner, Elisabeth; Peterson, Darryl; Kanwar, Yashpal S.

In: American Journal of Physiology - Renal Physiology, Vol. 279, No. 4 48-4, 07.12.2000.

Research output: Contribution to journalArticle

Yang, Q, Tian, Y, Wada, J, Kashihara, N, Wallner, E, Peterson, D & Kanwar, YS 2000, 'Expression characteristics and relevance of sodium glucose cotransporter-1 in mammalian renal tubulogenesis', American Journal of Physiology - Renal Physiology, vol. 279, no. 4 48-4.
Yang, Qiwei ; Tian, Yufeng ; Wada, Jun ; Kashihara, Naoki ; Wallner, Elisabeth ; Peterson, Darryl ; Kanwar, Yashpal S. / Expression characteristics and relevance of sodium glucose cotransporter-1 in mammalian renal tubulogenesis. In: American Journal of Physiology - Renal Physiology. 2000 ; Vol. 279, No. 4 48-4.
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