Doublesex (DSX) is a transcription factor in Drosophila melanogaster that regulates somatic sexual differentiation. Homologues have been found in diverse metazoans, suggesting a conserved role in development. Here, the expression, purification and crystallization of a novel 63-amino-acid dimerization domain and selenomethionine analogue are described. Native crystals belong to space group P212121 and diffract to a resolution of 1.6 Å with synchrotron radiation. Selenomethionine-containing crystals provide anomalous dispersion for phasing.
|Original language||English (US)|
|Number of pages||3|
|Journal||Acta Crystallographica Section D: Biological Crystallography|
|State||Published - Jul 2004|
ASJC Scopus subject areas
- Structural Biology