Expression, crystallization and preliminary X-ray characterization of the Drosophila transcription factor Doublesex

James Bayrer, Zhuli Wan, Biaoru Li, Michael A. Weiss

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2 Scopus citations


Doublesex (DSX) is a transcription factor in Drosophila melanogaster that regulates somatic sexual differentiation. Homologues have been found in diverse metazoans, suggesting a conserved role in development. Here, the expression, purification and crystallization of a novel 63-amino-acid dimerization domain and selenomethionine analogue are described. Native crystals belong to space group P212121 and diffract to a resolution of 1.6 Å with synchrotron radiation. Selenomethionine-containing crystals provide anomalous dispersion for phasing.

Original languageEnglish (US)
Pages (from-to)1328-1330
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Issue number7
StatePublished - Jul 1 2004
Externally publishedYes


ASJC Scopus subject areas

  • Structural Biology

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