Expression, crystallization and preliminary X-ray characterization of the Drosophila transcription factor Doublesex

James Bayrer, Zhuli Wan, Biaoru Li, Michael A. Weiss

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Doublesex (DSX) is a transcription factor in Drosophila melanogaster that regulates somatic sexual differentiation. Homologues have been found in diverse metazoans, suggesting a conserved role in development. Here, the expression, purification and crystallization of a novel 63-amino-acid dimerization domain and selenomethionine analogue are described. Native crystals belong to space group P212121 and diffract to a resolution of 1.6 Å with synchrotron radiation. Selenomethionine-containing crystals provide anomalous dispersion for phasing.

Original languageEnglish (US)
Pages (from-to)1328-1330
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume60
Issue number7
DOIs
StatePublished - Jul 1 2004

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Selenomethionine
Crystallization
Drosophila
Transcription Factors
X-Rays
Sex Differentiation
Synchrotrons
Dimerization
Drosophila melanogaster
Radiation
Amino Acids

ASJC Scopus subject areas

  • Structural Biology

Cite this

Expression, crystallization and preliminary X-ray characterization of the Drosophila transcription factor Doublesex. / Bayrer, James; Wan, Zhuli; Li, Biaoru; Weiss, Michael A.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 60, No. 7, 01.07.2004, p. 1328-1330.

Research output: Contribution to journalArticle

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