Expression of a unique globo-series glycolipid in cultured rat dorsal root ganglion neurons: Relationship with neuronal development

Shubhro Pal, John W. Bigbee, Megumi Saito, Toshio Ariga, Robert K Yu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Previous studies from this laboratory demonstrated the presence of a UDP- galactose:Gb3Cer α1-3galactosyltansferase activity responsible for the synthesis of a unique glycosphingolipid (GSL), Galα1-3Gb3Cer, in cultured PC12 pheochromocytoma cells (21). In this investigation, we examined the presence of this enzyme activity in isolated rat embryonic dorsal root ganglion neurons (DRGN), which, like pheochromocytoma cells, originate from the neural crest cells. DRGN exhibited the α-galactosyltransferase activity and the activity was comparable to that of the PC12 cells while several other rat tissues, with the exception of kidney, showed minimal activity. In order to define the spatial and temporal expression of Galα1-3Gb3Cer in DRGN, we examined the expression of Galα1-3Gb3Cer in cultured DRGN derived from embryonic day 16 rat embryos. Using a polyclonal antibody raised against Galα1-3Gb3Cer, we examined the localization of this glycolipid in DRGN cells after 5, 8, 12, and 15 days in culture. Immunostaining was restricted to the neurons while Schwann cells were negative. At day 5, the immunostaining was weak and confined to the cell body of the DRGN, though neurites were present at this stage. The period between days 5 and 15 represented a period of rapid neuritic growth and continued enlargement of the cell bodies. Immunoreactivity in the cell bodies increased dramatically by day 8. By day 12, immunoreactivity was present in neurites, and by day 15, was strong in both cell bodies and neurites. The expression of Galα1-3Gb3Cer in vivo was confirmed by immunostaining of frozen sections of dorsal root ganglia. Our present studies which demonstrate neuron-specific expression of Galα1- 3Gb3Cer during neurotigenesis combined with previous observations for its expression in PC12 cells, strongly implicates this GSL in neuronal development.

Original languageEnglish (US)
Pages (from-to)403-409
Number of pages7
JournalNeurochemical Research
Volume21
Issue number4
DOIs
StatePublished - Apr 1 1996
Externally publishedYes

Fingerprint

Glycolipids
Spinal Ganglia
Neurons
Rats
Cells
PC12 Cells
Neurites
Glycosphingolipids
Pheochromocytoma
Uridine Diphosphate Galactose
Galactosyltransferases
Neural Crest
Schwann Cells
Frozen Sections
Enzyme activity
Cultured Cells
Embryonic Structures
Tissue
Kidney
Cell Body

Keywords

  • dorsal rest ganglion
  • Glycosphingolipid
  • neuronal development

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Expression of a unique globo-series glycolipid in cultured rat dorsal root ganglion neurons : Relationship with neuronal development. / Pal, Shubhro; Bigbee, John W.; Saito, Megumi; Ariga, Toshio; Yu, Robert K.

In: Neurochemical Research, Vol. 21, No. 4, 01.04.1996, p. 403-409.

Research output: Contribution to journalArticle

@article{a4ca94234ab34285a947351f95fc21be,
title = "Expression of a unique globo-series glycolipid in cultured rat dorsal root ganglion neurons: Relationship with neuronal development",
abstract = "Previous studies from this laboratory demonstrated the presence of a UDP- galactose:Gb3Cer α1-3galactosyltansferase activity responsible for the synthesis of a unique glycosphingolipid (GSL), Galα1-3Gb3Cer, in cultured PC12 pheochromocytoma cells (21). In this investigation, we examined the presence of this enzyme activity in isolated rat embryonic dorsal root ganglion neurons (DRGN), which, like pheochromocytoma cells, originate from the neural crest cells. DRGN exhibited the α-galactosyltransferase activity and the activity was comparable to that of the PC12 cells while several other rat tissues, with the exception of kidney, showed minimal activity. In order to define the spatial and temporal expression of Galα1-3Gb3Cer in DRGN, we examined the expression of Galα1-3Gb3Cer in cultured DRGN derived from embryonic day 16 rat embryos. Using a polyclonal antibody raised against Galα1-3Gb3Cer, we examined the localization of this glycolipid in DRGN cells after 5, 8, 12, and 15 days in culture. Immunostaining was restricted to the neurons while Schwann cells were negative. At day 5, the immunostaining was weak and confined to the cell body of the DRGN, though neurites were present at this stage. The period between days 5 and 15 represented a period of rapid neuritic growth and continued enlargement of the cell bodies. Immunoreactivity in the cell bodies increased dramatically by day 8. By day 12, immunoreactivity was present in neurites, and by day 15, was strong in both cell bodies and neurites. The expression of Galα1-3Gb3Cer in vivo was confirmed by immunostaining of frozen sections of dorsal root ganglia. Our present studies which demonstrate neuron-specific expression of Galα1- 3Gb3Cer during neurotigenesis combined with previous observations for its expression in PC12 cells, strongly implicates this GSL in neuronal development.",
keywords = "dorsal rest ganglion, Glycosphingolipid, neuronal development",
author = "Shubhro Pal and Bigbee, {John W.} and Megumi Saito and Toshio Ariga and Yu, {Robert K}",
year = "1996",
month = "4",
day = "1",
doi = "10.1007/BF02527703",
language = "English (US)",
volume = "21",
pages = "403--409",
journal = "Neurochemical Research",
issn = "0364-3190",
publisher = "Springer New York",
number = "4",

}

TY - JOUR

T1 - Expression of a unique globo-series glycolipid in cultured rat dorsal root ganglion neurons

T2 - Relationship with neuronal development

AU - Pal, Shubhro

AU - Bigbee, John W.

AU - Saito, Megumi

AU - Ariga, Toshio

AU - Yu, Robert K

PY - 1996/4/1

Y1 - 1996/4/1

N2 - Previous studies from this laboratory demonstrated the presence of a UDP- galactose:Gb3Cer α1-3galactosyltansferase activity responsible for the synthesis of a unique glycosphingolipid (GSL), Galα1-3Gb3Cer, in cultured PC12 pheochromocytoma cells (21). In this investigation, we examined the presence of this enzyme activity in isolated rat embryonic dorsal root ganglion neurons (DRGN), which, like pheochromocytoma cells, originate from the neural crest cells. DRGN exhibited the α-galactosyltransferase activity and the activity was comparable to that of the PC12 cells while several other rat tissues, with the exception of kidney, showed minimal activity. In order to define the spatial and temporal expression of Galα1-3Gb3Cer in DRGN, we examined the expression of Galα1-3Gb3Cer in cultured DRGN derived from embryonic day 16 rat embryos. Using a polyclonal antibody raised against Galα1-3Gb3Cer, we examined the localization of this glycolipid in DRGN cells after 5, 8, 12, and 15 days in culture. Immunostaining was restricted to the neurons while Schwann cells were negative. At day 5, the immunostaining was weak and confined to the cell body of the DRGN, though neurites were present at this stage. The period between days 5 and 15 represented a period of rapid neuritic growth and continued enlargement of the cell bodies. Immunoreactivity in the cell bodies increased dramatically by day 8. By day 12, immunoreactivity was present in neurites, and by day 15, was strong in both cell bodies and neurites. The expression of Galα1-3Gb3Cer in vivo was confirmed by immunostaining of frozen sections of dorsal root ganglia. Our present studies which demonstrate neuron-specific expression of Galα1- 3Gb3Cer during neurotigenesis combined with previous observations for its expression in PC12 cells, strongly implicates this GSL in neuronal development.

AB - Previous studies from this laboratory demonstrated the presence of a UDP- galactose:Gb3Cer α1-3galactosyltansferase activity responsible for the synthesis of a unique glycosphingolipid (GSL), Galα1-3Gb3Cer, in cultured PC12 pheochromocytoma cells (21). In this investigation, we examined the presence of this enzyme activity in isolated rat embryonic dorsal root ganglion neurons (DRGN), which, like pheochromocytoma cells, originate from the neural crest cells. DRGN exhibited the α-galactosyltransferase activity and the activity was comparable to that of the PC12 cells while several other rat tissues, with the exception of kidney, showed minimal activity. In order to define the spatial and temporal expression of Galα1-3Gb3Cer in DRGN, we examined the expression of Galα1-3Gb3Cer in cultured DRGN derived from embryonic day 16 rat embryos. Using a polyclonal antibody raised against Galα1-3Gb3Cer, we examined the localization of this glycolipid in DRGN cells after 5, 8, 12, and 15 days in culture. Immunostaining was restricted to the neurons while Schwann cells were negative. At day 5, the immunostaining was weak and confined to the cell body of the DRGN, though neurites were present at this stage. The period between days 5 and 15 represented a period of rapid neuritic growth and continued enlargement of the cell bodies. Immunoreactivity in the cell bodies increased dramatically by day 8. By day 12, immunoreactivity was present in neurites, and by day 15, was strong in both cell bodies and neurites. The expression of Galα1-3Gb3Cer in vivo was confirmed by immunostaining of frozen sections of dorsal root ganglia. Our present studies which demonstrate neuron-specific expression of Galα1- 3Gb3Cer during neurotigenesis combined with previous observations for its expression in PC12 cells, strongly implicates this GSL in neuronal development.

KW - dorsal rest ganglion

KW - Glycosphingolipid

KW - neuronal development

UR - http://www.scopus.com/inward/record.url?scp=0029904175&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029904175&partnerID=8YFLogxK

U2 - 10.1007/BF02527703

DO - 10.1007/BF02527703

M3 - Article

C2 - 8734432

AN - SCOPUS:0029904175

VL - 21

SP - 403

EP - 409

JO - Neurochemical Research

JF - Neurochemical Research

SN - 0364-3190

IS - 4

ER -