Expression of caspase-14 reduces tumorigenicity of skin cancer cells

Stephen Hsu, Haiyan Qin, Douglas Dickinson, Ding Xie, Wendy B Bollag, Hubert Stöppler, Henna Pearl, Anna Vu, Margaretta Watkins, Meredith Koehler, George Schuster

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: The green tea polyphenol (-)-epigallocatechin-3-gallate (EGCG) possesses anti-carcinogenic properties and was found to induce terminal differentiation in epidermal keratinocytes. Caspase-14, a member of the caspase family associated with epithelial cell differentiation, planned cell death, and barrier formation, is induced by EGCG in normal human epidermal keratinocytes but not in cancer cells. Materials and methods: A human epidermoid cancer cell line, A431, was co-transfected with a caspase-14-expressing pCMV vector and a GFP/ neo-expressing pCMV vector. Cell growth and tumorigenicity of the stable transfectant were determined in comparison to cells transfected with the control GFP/neo-expressing pCMV vector. Results: Expression of exogenous caspase-14 led to growth inhibition and reduced the tumorigenicity of A431 cells. Conclusion: Pending future studies, caspase-14 could be used as a novel approach to skin cancer therapy via gene delivery systems.

Original languageEnglish (US)
Pages (from-to)279-283
Number of pages5
JournalIn Vivo
Volume21
Issue number2
StatePublished - Mar 1 2007

Fingerprint

Caspase 14
Skin Neoplasms
Skin
Cells
Keratinocytes
Gene Transfer Techniques
Cell growth
Polyphenols
Tea
Cell death
Growth
Caspases
Cell Differentiation
Neoplasms
Cell Death
Genes
Epithelial Cells
Cell Line

Keywords

  • Caspase-14, A431
  • EGCG
  • Green tea polyphenols
  • Skin cancer

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology

Cite this

Hsu, S., Qin, H., Dickinson, D., Xie, D., Bollag, W. B., Stöppler, H., ... Schuster, G. (2007). Expression of caspase-14 reduces tumorigenicity of skin cancer cells. In Vivo, 21(2), 279-283.

Expression of caspase-14 reduces tumorigenicity of skin cancer cells. / Hsu, Stephen; Qin, Haiyan; Dickinson, Douglas; Xie, Ding; Bollag, Wendy B; Stöppler, Hubert; Pearl, Henna; Vu, Anna; Watkins, Margaretta; Koehler, Meredith; Schuster, George.

In: In Vivo, Vol. 21, No. 2, 01.03.2007, p. 279-283.

Research output: Contribution to journalArticle

Hsu, S, Qin, H, Dickinson, D, Xie, D, Bollag, WB, Stöppler, H, Pearl, H, Vu, A, Watkins, M, Koehler, M & Schuster, G 2007, 'Expression of caspase-14 reduces tumorigenicity of skin cancer cells', In Vivo, vol. 21, no. 2, pp. 279-283.
Hsu S, Qin H, Dickinson D, Xie D, Bollag WB, Stöppler H et al. Expression of caspase-14 reduces tumorigenicity of skin cancer cells. In Vivo. 2007 Mar 1;21(2):279-283.
Hsu, Stephen ; Qin, Haiyan ; Dickinson, Douglas ; Xie, Ding ; Bollag, Wendy B ; Stöppler, Hubert ; Pearl, Henna ; Vu, Anna ; Watkins, Margaretta ; Koehler, Meredith ; Schuster, George. / Expression of caspase-14 reduces tumorigenicity of skin cancer cells. In: In Vivo. 2007 ; Vol. 21, No. 2. pp. 279-283.
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AB - Background: The green tea polyphenol (-)-epigallocatechin-3-gallate (EGCG) possesses anti-carcinogenic properties and was found to induce terminal differentiation in epidermal keratinocytes. Caspase-14, a member of the caspase family associated with epithelial cell differentiation, planned cell death, and barrier formation, is induced by EGCG in normal human epidermal keratinocytes but not in cancer cells. Materials and methods: A human epidermoid cancer cell line, A431, was co-transfected with a caspase-14-expressing pCMV vector and a GFP/ neo-expressing pCMV vector. Cell growth and tumorigenicity of the stable transfectant were determined in comparison to cells transfected with the control GFP/neo-expressing pCMV vector. Results: Expression of exogenous caspase-14 led to growth inhibition and reduced the tumorigenicity of A431 cells. Conclusion: Pending future studies, caspase-14 could be used as a novel approach to skin cancer therapy via gene delivery systems.

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