Expression of extracellular matrix components in a highly infiltrative in vivo glioma model

Rupavathana Mahesparan, Tracy Ann Read, Morten Lund-Johansen, Kai Ove Skaftnesmo, Rolf Bjerkvig, Olav Engebraaten

Research output: Contribution to journalArticlepeer-review

127 Scopus citations

Abstract

This work demonstrates the expression of extracellular matrix (ECM) components in a highly infiltrative brain tumor model developed by simple inoculation of spheroids from five human glioma biopsy tissues directly into the brains of immunodeficient rats. Non-invasive tumors derived from one glioblastoma biopsy specimen and two glioma cell lines (D-54MG and U-251MG) were also included in this study. The extent of tumor cell infiltration was studied using a pan-human monoclonal anti-vimentin antibody. The cellular origin for several of these ECM components was identified using human-specific monoclonal antibodies and polyclonal antibodies detecting epitopes trom both species. Immunostaining revealed a diffuse parenchymal staining of glioma-produced tenascin, whereas vitronectin was produced mainly by the invading glioma cells. ECM components such as laminin, fibronectin and collagen type TV were most probably produced by the host and were mainly associated with the blood vessels in the tumors. However, some parenchymal staining with regional variations was observed. The expression pattern of these components was different in cell lines tumors as compared to the biopsy specimen tumors. The α3 and β1 integrin subunits were mainly observed in areas of tumor cell invasion in the invasive tumors. In conclusion, the observed staining patterns clarify the cellular origin and indicate the possible biological function of tenascin, vitronectin, laminin, fibronectin and collagen type TV in these highly invasive malignant tumors of glial origin.

Original languageEnglish (US)
Pages (from-to)49-57
Number of pages9
JournalActa Neuropathologica
Volume105
Issue number1
DOIs
StatePublished - Jan 1 2003
Externally publishedYes

Keywords

  • Biopsy
  • Extracellular matrix components
  • Glioma
  • Invasion
  • Nude rat model

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

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