Expression of functional human recombinant interleukin-12 and development of a new reliable bioassay by interferon-γ elispot

Yan Cui, Lung Ji Chang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Interleukin-12 (IL-12) is one of the important cytokines for promoting the differentiation and maturation of type 1 T helper cells and facilitating the initiation of cell-mediated immune responses. Because of its multi-functional roles in anti-tumor and anti-viral immunity, IL-12 is considered a promising therapeutic cytokine in immunotherapy against cancer and infectious diseases. To evaluate the therapeutic efficacy, an easy and reliable quantitative assay for functional IL-12 is essential. Presently, IL-12 concentration is often determined by ELISA or RIA, which may or may not correlate with IL-12 biological function. Established IL-12 bioassays are based on a time-consuming lymphocyte proliferation assay and require freshly isolated peripheral blood lymphocytes.

Original languageEnglish (US)
Pages (from-to)23-36
Number of pages14
JournalPreparative Biochemistry and Biotechnology
Volume28
Issue number1
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

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Bioassay
Interleukin-12
Biological Assay
Interferons
Lymphocytes
Assays
Cytokines
Th1 Cells
Immunotherapy
Communicable Diseases
Tumors
Immunity
Neoplasms
Blood
Enzyme-Linked Immunosorbent Assay
Therapeutics

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry

Cite this

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abstract = "Interleukin-12 (IL-12) is one of the important cytokines for promoting the differentiation and maturation of type 1 T helper cells and facilitating the initiation of cell-mediated immune responses. Because of its multi-functional roles in anti-tumor and anti-viral immunity, IL-12 is considered a promising therapeutic cytokine in immunotherapy against cancer and infectious diseases. To evaluate the therapeutic efficacy, an easy and reliable quantitative assay for functional IL-12 is essential. Presently, IL-12 concentration is often determined by ELISA or RIA, which may or may not correlate with IL-12 biological function. Established IL-12 bioassays are based on a time-consuming lymphocyte proliferation assay and require freshly isolated peripheral blood lymphocytes.",
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