Expression of GD2 and GD3 gangliosides in human embryonic neural stem cells

Makoto Yanagisawa, Saori Yoshimura, Robert K. Yu

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

NSCs (neural stem cells) are undifferentiated neural cells endowed with a high potential for proliferation and a capacity for self-renewal with retention of multipotency to differentiate into neurons and glial cells. It has been recently reported that GD3, a b-series ganglioside, is a marker molecule for identifying and isolating mouse NSCs. However, the expression of gangliosides in human NSCs is largely unknown. In the present study, we analysed the expression of gangliosides, GD2 and GD3, in human NSCs that were isolated from human brains at gestational week 17 in the form of neurospheres, which are floating clonal aggregates formed by NSCs in vitro. Employing immunocytochemistry, we found that human NSCs were strongly reactive to anti-GD2 antibody and relatively weakly reactive to anti-GD3 antibody. Treatment of these cells with an organic solvent such as 100% methanol, which selectively removes glycolipids from plasma membrane, abolished the immunoreactivity with those antibodies, indicating that the reactivity was due to GD2 and GD3, but not to GD2-/GD3- like glycoproteins or proteoglycans. The immunoreactivity of human NSCs to antibody against SSEA-1 (stage-specific embryonic antigen-1), a well-known carbohydrate antigen of NSCs, was not decreased by the treatment with 100% methanol, indicating that SSEA-1 is mainly carried by glycoproteins and/or proteoglycans in human NSCs. Our study suggests that GD2 and GD3 can be marker gangliosides for identifying human NSCs.

Original languageEnglish (US)
JournalASN Neuro
Volume3
Issue number2
DOIs
StatePublished - Apr 7 2011

Fingerprint

Neural Stem Cells
Gangliosides
CD15 Antigens
Proteoglycans
Methanol
Anti-Idiotypic Antibodies
Glycoproteins
GD2 ganglioside
GD3 ganglioside
Human Embryonic Stem Cells
Antibodies
Glycolipids
Neuroglia
Immunohistochemistry
Carbohydrates
Cell Membrane
Neurons
Antigens

Keywords

  • Ganglioside
  • Glycosphingolipid (GSL)
  • Neural stem cell (NSC)
  • Neurosphere
  • Stage-specific embryonic antigen-1 (SSEA-1)

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology

Cite this

Expression of GD2 and GD3 gangliosides in human embryonic neural stem cells. / Yanagisawa, Makoto; Yoshimura, Saori; Yu, Robert K.

In: ASN Neuro, Vol. 3, No. 2, 07.04.2011.

Research output: Contribution to journalArticle

Yanagisawa, Makoto ; Yoshimura, Saori ; Yu, Robert K. / Expression of GD2 and GD3 gangliosides in human embryonic neural stem cells. In: ASN Neuro. 2011 ; Vol. 3, No. 2.
@article{43e31f4a6330418686ce24ac7e690262,
title = "Expression of GD2 and GD3 gangliosides in human embryonic neural stem cells",
abstract = "NSCs (neural stem cells) are undifferentiated neural cells endowed with a high potential for proliferation and a capacity for self-renewal with retention of multipotency to differentiate into neurons and glial cells. It has been recently reported that GD3, a b-series ganglioside, is a marker molecule for identifying and isolating mouse NSCs. However, the expression of gangliosides in human NSCs is largely unknown. In the present study, we analysed the expression of gangliosides, GD2 and GD3, in human NSCs that were isolated from human brains at gestational week 17 in the form of neurospheres, which are floating clonal aggregates formed by NSCs in vitro. Employing immunocytochemistry, we found that human NSCs were strongly reactive to anti-GD2 antibody and relatively weakly reactive to anti-GD3 antibody. Treatment of these cells with an organic solvent such as 100{\%} methanol, which selectively removes glycolipids from plasma membrane, abolished the immunoreactivity with those antibodies, indicating that the reactivity was due to GD2 and GD3, but not to GD2-/GD3- like glycoproteins or proteoglycans. The immunoreactivity of human NSCs to antibody against SSEA-1 (stage-specific embryonic antigen-1), a well-known carbohydrate antigen of NSCs, was not decreased by the treatment with 100{\%} methanol, indicating that SSEA-1 is mainly carried by glycoproteins and/or proteoglycans in human NSCs. Our study suggests that GD2 and GD3 can be marker gangliosides for identifying human NSCs.",
keywords = "Ganglioside, Glycosphingolipid (GSL), Neural stem cell (NSC), Neurosphere, Stage-specific embryonic antigen-1 (SSEA-1)",
author = "Makoto Yanagisawa and Saori Yoshimura and Yu, {Robert K.}",
year = "2011",
month = "4",
day = "7",
doi = "10.1042/AN20110006",
language = "English (US)",
volume = "3",
journal = "ASN Neuro",
issn = "1759-0914",
publisher = "Portland Press Ltd.",
number = "2",

}

TY - JOUR

T1 - Expression of GD2 and GD3 gangliosides in human embryonic neural stem cells

AU - Yanagisawa, Makoto

AU - Yoshimura, Saori

AU - Yu, Robert K.

PY - 2011/4/7

Y1 - 2011/4/7

N2 - NSCs (neural stem cells) are undifferentiated neural cells endowed with a high potential for proliferation and a capacity for self-renewal with retention of multipotency to differentiate into neurons and glial cells. It has been recently reported that GD3, a b-series ganglioside, is a marker molecule for identifying and isolating mouse NSCs. However, the expression of gangliosides in human NSCs is largely unknown. In the present study, we analysed the expression of gangliosides, GD2 and GD3, in human NSCs that were isolated from human brains at gestational week 17 in the form of neurospheres, which are floating clonal aggregates formed by NSCs in vitro. Employing immunocytochemistry, we found that human NSCs were strongly reactive to anti-GD2 antibody and relatively weakly reactive to anti-GD3 antibody. Treatment of these cells with an organic solvent such as 100% methanol, which selectively removes glycolipids from plasma membrane, abolished the immunoreactivity with those antibodies, indicating that the reactivity was due to GD2 and GD3, but not to GD2-/GD3- like glycoproteins or proteoglycans. The immunoreactivity of human NSCs to antibody against SSEA-1 (stage-specific embryonic antigen-1), a well-known carbohydrate antigen of NSCs, was not decreased by the treatment with 100% methanol, indicating that SSEA-1 is mainly carried by glycoproteins and/or proteoglycans in human NSCs. Our study suggests that GD2 and GD3 can be marker gangliosides for identifying human NSCs.

AB - NSCs (neural stem cells) are undifferentiated neural cells endowed with a high potential for proliferation and a capacity for self-renewal with retention of multipotency to differentiate into neurons and glial cells. It has been recently reported that GD3, a b-series ganglioside, is a marker molecule for identifying and isolating mouse NSCs. However, the expression of gangliosides in human NSCs is largely unknown. In the present study, we analysed the expression of gangliosides, GD2 and GD3, in human NSCs that were isolated from human brains at gestational week 17 in the form of neurospheres, which are floating clonal aggregates formed by NSCs in vitro. Employing immunocytochemistry, we found that human NSCs were strongly reactive to anti-GD2 antibody and relatively weakly reactive to anti-GD3 antibody. Treatment of these cells with an organic solvent such as 100% methanol, which selectively removes glycolipids from plasma membrane, abolished the immunoreactivity with those antibodies, indicating that the reactivity was due to GD2 and GD3, but not to GD2-/GD3- like glycoproteins or proteoglycans. The immunoreactivity of human NSCs to antibody against SSEA-1 (stage-specific embryonic antigen-1), a well-known carbohydrate antigen of NSCs, was not decreased by the treatment with 100% methanol, indicating that SSEA-1 is mainly carried by glycoproteins and/or proteoglycans in human NSCs. Our study suggests that GD2 and GD3 can be marker gangliosides for identifying human NSCs.

KW - Ganglioside

KW - Glycosphingolipid (GSL)

KW - Neural stem cell (NSC)

KW - Neurosphere

KW - Stage-specific embryonic antigen-1 (SSEA-1)

UR - http://www.scopus.com/inward/record.url?scp=79958114113&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79958114113&partnerID=8YFLogxK

U2 - 10.1042/AN20110006

DO - 10.1042/AN20110006

M3 - Article

C2 - 21395555

AN - SCOPUS:79958114113

VL - 3

JO - ASN Neuro

JF - ASN Neuro

SN - 1759-0914

IS - 2

ER -