Expression of glutamate receptor subunit/subtype messenger RNAS for NMDAR1, GLuR1, GLuR2 and mGLuR5 by accumbal projection neurons

Xinyun Lu, M. Behnam Ghasemzadeh, Peter W. Kalivas

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Nucleus accumbens neurons are the targets of glutamatergic inputs. By coupling in situ hybridization for glutamate receptor mRNAs with retrograde transport of Fluoro-Gold, the present study examined the relationship between the distribution patterns of glutamate receptor subtypes/subunits and the output pathways of the nucleus accumbens to the ventral pallidum and ventral tegmental area. Following iontophoretic deposits of Fluoro-Gold into the ventral pallidum, neurons in both the nucleus accumbens shell and core were retrogradely labeled. A high percentage of accumbens neurons retrogradely labeled from the ventral pallidum were double-labeled for mRNAs encoding for mGluR5 (82 ± 4.1%), NMDAR1 (71 ± 3.5%), GluR1 (70 ± 6.1%) and GluR2 (76 ± 3.6%). No significant difference in the proportion of double-labeled neurons between the core and shell was observed. Following the deposit of Fluoro- Gold into the ventral tegmental area, only the accumbens shell neurons were retrogradely labeled. The proportion of neurons expressing NMDAR1, GluR1 and GluR2 were somewhat less in the projection to the ventral tegmental area compared to the ventral pallidum since approximately 60% of the neurons retrogradely-labeled from the ventral tegmental area expressed these transcripts. In contrast to the high proportion of mGluR5-containing neurons in the nucleus accumbens innervating the ventral pallidum, only half of the neurons projecting to the ventral tegmental area expressed mGluR5. These data show that accumbens neurons innervating the ventral pallidum and ventral tegmental area differ in the relative proportion of expressed mRNA encoding mGluR5, implying differential postsynaptic impact by glutamate transmission on neurons contributing to the two major efferent pathways of the nucleus accumbens.

Original languageEnglish (US)
Pages (from-to)287-296
Number of pages10
JournalMolecular Brain Research
Volume63
Issue number2
DOIs
StatePublished - Jan 8 1999
Externally publishedYes

Fingerprint

Glutamate Receptors
Ventral Tegmental Area
Neurons
Nucleus Accumbens
Messenger RNA
NMDA receptor A1
Efferent Pathways
In Situ Hybridization
Basal Forebrain
Glutamic Acid

Keywords

  • GluR1
  • GluR2
  • Glutamate
  • MGluR5
  • NMDAR1
  • Nucleus accumbens

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Expression of glutamate receptor subunit/subtype messenger RNAS for NMDAR1, GLuR1, GLuR2 and mGLuR5 by accumbal projection neurons. / Lu, Xinyun; Behnam Ghasemzadeh, M.; Kalivas, Peter W.

In: Molecular Brain Research, Vol. 63, No. 2, 08.01.1999, p. 287-296.

Research output: Contribution to journalArticle

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abstract = "Nucleus accumbens neurons are the targets of glutamatergic inputs. By coupling in situ hybridization for glutamate receptor mRNAs with retrograde transport of Fluoro-Gold, the present study examined the relationship between the distribution patterns of glutamate receptor subtypes/subunits and the output pathways of the nucleus accumbens to the ventral pallidum and ventral tegmental area. Following iontophoretic deposits of Fluoro-Gold into the ventral pallidum, neurons in both the nucleus accumbens shell and core were retrogradely labeled. A high percentage of accumbens neurons retrogradely labeled from the ventral pallidum were double-labeled for mRNAs encoding for mGluR5 (82 ± 4.1{\%}), NMDAR1 (71 ± 3.5{\%}), GluR1 (70 ± 6.1{\%}) and GluR2 (76 ± 3.6{\%}). No significant difference in the proportion of double-labeled neurons between the core and shell was observed. Following the deposit of Fluoro- Gold into the ventral tegmental area, only the accumbens shell neurons were retrogradely labeled. The proportion of neurons expressing NMDAR1, GluR1 and GluR2 were somewhat less in the projection to the ventral tegmental area compared to the ventral pallidum since approximately 60{\%} of the neurons retrogradely-labeled from the ventral tegmental area expressed these transcripts. In contrast to the high proportion of mGluR5-containing neurons in the nucleus accumbens innervating the ventral pallidum, only half of the neurons projecting to the ventral tegmental area expressed mGluR5. These data show that accumbens neurons innervating the ventral pallidum and ventral tegmental area differ in the relative proportion of expressed mRNA encoding mGluR5, implying differential postsynaptic impact by glutamate transmission on neurons contributing to the two major efferent pathways of the nucleus accumbens.",
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